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Preparation method of hoof nail polypeptide through composite hydrolysis

A technology of compound hydrolysis and hoof nails, applied in the directions of hydrolyzed protein components, peptides, drug combinations, etc., can solve the problems of unfavorable actual curative effect, improvement, and no drug effect.

Active Publication Date: 2011-09-21
SHANDONG LUBEI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, this method is the same as the traditional acid or alkali hydrolysis method. While simply pursuing the improvement of hoof nail hydrolysis rate, it ignores the comprehensive investigation of drug components and activities. Although the hydrolysis rate has a certain increase, it does not bring about The improvement of the drug effect, on the contrary, because the amino nitrogen in its products accounts for more than 4% of the total nitrogen content, which means that too many polypeptide molecules are over-hydrolyzed into amino acids that do not contribute much to the therapeutic effect, which is not good for the actual curative effect.

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  • Preparation method of hoof nail polypeptide through composite hydrolysis

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Embodiment 1

[0021] 1) Washing, pyrolyzing, drying and pulverizing the raw hoof shells to obtain raw material powder;

[0022] 2) Put the raw material powder in a NaOH solution whose weight is 20 times that of the raw material dry powder and whose pH is 8.6, stir evenly at a temperature of 86°C, and perform alkaline hydrolysis for 11 hours;

[0023] 3) After the hydrolysis mixture is filtered to remove the coarse residue, then the filtrate is ultrafiltered with an ultrafiltration membrane with a molecular weight not greater than 30,000 Daltons, and the obtained filtrate is subjected to enzymatic hydrolysis; the filtered residue filtered out twice is returned to the NaOH solution for further processing alkaline hydrolysis;

[0024] 4) in the filtrate of ultrafiltration gained, add the papain (enzyme activity 1000 μ / mg) that weight is raw material dry powder 0.15% and the serrapeptase (enzyme activity 300,000 μ / g) that weight is raw material dry powder 0.2%, adjust The pH of the solution is...

Embodiment 2

[0030] 1) Washing, pyrolyzing, drying and pulverizing the raw hoof shells to obtain raw material powder;

[0031] 2) Put the raw material powder in a NaOH solution whose weight is 22 times that of the raw material dry powder and whose pH is 8.4, stir evenly at a temperature of 84°C, and perform alkaline hydrolysis for 12 hours;

[0032] 3) After the hydrolysis mixture is filtered to remove the coarse residue, then the filtrate is ultrafiltered with an ultrafiltration membrane with a molecular weight not greater than 30,000 Daltons, and the obtained filtrate is subjected to enzymatic hydrolysis; the filtered residue filtered out twice is returned to the NaOH solution for further processing alkaline hydrolysis;

[0033] 4) in the filtrate of ultrafiltration gained, add the papain (enzyme activity 1000 μ / mg) that weight is raw material dry powder 0.2% and the serrapeptase (enzyme activity 300,000 μ / g) that weight is raw material dry powder 0.15%, adjust The pH of the solution is...

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Abstract

The invention provides a preparation method of hoof nail polypeptide through composite hydrolysis. The method comprises the following steps of: mildly and partially hydrolyzing a dried and crushed hoof nail raw material in aqueous alkali; and further hydrolyzing the hoof nail raw material with a proteolytic enzyme to obtain the hoof nail polypeptide product. In the hoof nail polypeptide prepared by the method, the content of the components of which the relative molecular mass is 10,000 to 3,500 is up to 50 percent, and the content of amino nitrogen is less than or equal to 3 percent of the content of total nitrogen. Compared with the prior art, the hoof nail polypeptide is obviously improved in cogulation effect and anti-inflammation effect.

Description

Technical field: [0001] The invention belongs to a method for preparing polypeptide biological medicines, in particular to a method for preparing hoof nail polypeptides by hydrolyzing pig hoof nails as raw materials. Background technique: [0002] Hoof polypeptide is a biologically active polypeptide component obtained from trotter hoof by hydrolysis. It has significant functions such as hemostasis, anti-inflammation, endocrine regulation, and improvement of body immunity. disease, has a unique therapeutic effect, and has no side effects, and has been widely used clinically. At present, the methods for producing hoof nail polypeptide are mainly acid hydrolysis or alkali hydrolysis, and the reports of alkali hydrolysis account for the majority. [0003] The most recent related report is "a method for preparing hoof nail polypeptide" disclosed in Chinese patent CN 101709076A on May 19, 2010. The method is an improved method for producing hoof nail polypeptide by alkali hydro...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00A61K38/01A61P7/04A61P29/00A61P5/00A61P37/04
Inventor 王克柳王美海杨红霞颜丙辉
Owner SHANDONG LUBEI PHARMA
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