Dihaploid induction method of head cabbage with high efficiency

A technology for head cabbage and double haploid, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problem of inability to meet large-scale embryo production and efficient breeding, low transplant survival rate, inability to produce embryos, etc. problems, to achieve the effect of eliminating vitrification problems, high embryo rate and cost saving

Inactive Publication Date: 2011-10-05
陕西省杂交油菜研究中心
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the microspore culture technology of head cabbage in my country is not yet mature enough, and most of them are in the digestion and absorption of related species culture technology and its limited improvement. The amount of embryos is very sm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dihaploid induction method of head cabbage with high efficiency
  • Dihaploid induction method of head cabbage with high efficiency
  • Dihaploid induction method of head cabbage with high efficiency

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] The formulations of the various media used in this example are shown in Table 1.

[0025] In this example, several headed cabbage genotypes Q2, Q4, Q5, Q8, HP63 and HW06 were selected as raw materials, and the induction and culture of headed cabbage double haploids were carried out according to the following steps. At the same time, a control group was established. No colchicine doubling agent in the start-up medium in the group, no paclobutrazol in the subculture and rooting medium:

[0026] (1) Selection of flower buds and separation of microspores

[0027] The cabbage to be grown in the artificial climate box, glass greenhouse and the natural environment of the field until the initial flowering stage, combined with microscopic observation, the flower bud size is in the range of 3.5-5.5mm, and it is determined that the proportion of microspore development in the mononuclear stage is up to 70% At the above, select the corresponding flower buds and put them ...

Example Embodiment

[0042] Example 2 In the process of separation, initiation and embryo induction, the concentration of sucrose in each medium was determined experiment

[0043] During the flowering period in mid-April 2007, take 20 portions of cabbage buds (bud size between 3.5-5.5mm) that have a mononuclear side stage microspore ratio of more than 70% in the field and put them in a steel basket for disinfection and sterilization. The microspores were crushed and washed in B5 liquid medium with a sucrose concentration of 150g / L, and the separated microspores were subjected to 33℃ in NLN liquid medium with sucrose concentrations of 130, 140, 150, 160, 170 and 180g / L. After high-temperature cultivation, 2 days later, observe and record the expansion of microspores, see Table 3.

[0044] Table 3 The influence of different sucrose concentrations in the NLN liquid medium during the start-up process on the development and expansion of microspores

[0045]

[0046] 140

[0047] It can be seen from Ta...

Example Embodiment

[0052] Example 3 Experiments on the appropriate concentration and treatment duration induced by colchicine synchronously doubling in the initial culture stage

[0053] The buds were taken from the field during the flowering period in April 2007 and 2008, separated and washed with B5 liquid medium with a concentration of 150g / L sucrose, initiated induction in NLN liquid medium with a concentration of 160g / L sucrose, and embryo induction culture in NLN liquid medium with a concentration of 140g / L sucrose Double induction is to add different concentrations of colchicine to the NLN liquid medium in the initial culture stage, and after treatment for a certain period of time (48 hours), the statistical expansion rate and the final embryo doubling situation are investigated. The results are shown in Table 5.

[0054] Table 5 Doubling of embryos treated with different concentrations of colchicine for 48 hours

[0055] Colchicine

[0056] Experiments have shown that colchicine can double e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a dihaploid induction method of head cabbage with high efficiency, which belongs to the filed of biological breeding. The method relates to a regeneration of microspore plant of the horticultural plant head cabbage and an improvement of dihaploid induction technology thereof. The method comprises the following steps: (1) selecting bud and separating microspore; (2) doubling inducing and culturing formed embryo; (3) differencing seedlings and subculturing; (4) cultivating strong seedling rooting. According to the invention, the dihaploid of head cabbage can be applied in a mass production with a large scale, a plurality of novel genetype pure lines can be provided for breeding. The method is affected slightly of the growth environment and genotype, and has the advantages of high embryo generation and embryo formation, good doubling effect, strong growth of seedlings plant, less subculture frequency, strong root and seedings after rooting and high survival rate of the field transplanting.

Description

technical field [0001] The invention relates to a method for inducing double haploid (DH) of plants, in particular to a regeneration method of horticultural crop head cabbage microspore plants and a method for efficiently inducing double haploid, and a culture medium thereof. Background technique [0002] Heading cabbage is a kind of edible vegetable with large cultivation area, wide adaptability and high nutritional value, which is deeply loved by the people. However, because it is native to the Mediterranean coast, domestic germplasm resources are scarce, and foreign varieties and parental resources with superior performance are not available. It is difficult to obtain, which has always restricted the development of the breeding of fine varieties of cabbage in my country. If double haploid breeding is adopted, it will inevitably create a new and effective way for the acquisition of fine germplasm resources and the cultivation of new varieties, and it will also greatly improv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 王灏赵小萍李殿荣田建华同晓丽
Owner 陕西省杂交油菜研究中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap