Method for extracting and separating viterxin and isovitexin from natural product

A technology of isovitexin and natural products, applied in the direction of organic chemistry, can solve the problems of no vitexin, isovitexin, complicated process, etc., and achieve the effect of low cost, simple process and free radical scavenging

Inactive Publication Date: 2011-10-19
SOUTH CHINA AGRI UNIV
View PDF2 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction and separation of vitexin and isovitexin from natural plants is generally performed by silica gel column chromatography, SephadexLH-20 column chromatography and preparative HPLC

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting and separating viterxin and isovitexin from natural product
  • Method for extracting and separating viterxin and isovitexin from natural product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of the ethanol crude extract of sandalwood leaves: crush the sandalwood leaves, add 10 times the weight and 80% ethanol aqueous solution, stir well, and use ultrasonic waves with a frequency of 20KHz to extract, and the number of ultrasonic extractions is 2~ 3 times, sonicate for 10 min each time, then extract at room temperature for 48 h, filter, and repeat the extraction of the filter residue three times, combine the filtrate, concentrate the filtrate, and evaporate the ethanol to obtain the crude ethanol extract;

[0049] (2) Liquid-liquid extraction separation and purification of crude ethanol extract of sandalwood leaves: extract the crude ethanol extract with petroleum ether, chloroform, and ethyl acetate in sequence until the ethyl acetate solution is colorless and transparent, collect ethyl acetate for extraction liquid, concentrated and dried to obtain ethyl acetate extract;

[0050] (3) The ethyl acetate extract was separated by silica gel colu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting and separating viterxin and isovitexin from a natural product. The method comprises the following steps: based on santalum album L leaves as raw materials, carrying out ultrasonic extraction and lixiviation on the santalum album L leaves by utilizing an alcohol solvent; then respectively extracting santalum album L leaf extract by adopting petroleum ether, chloroform and ethyl acetate in turn; carrying out column chromatography on ethyl acetate extract, and recrystallizing the obtained eluent so as to obtain the isovitexin; and separating a residual liquid through a preparation liquid phase so as to obtain the viterxin. In the method, the santalum album L leaves are used for the first time so as to extract out the viterxin and isovitexin, the separation process is simple, the cost is low, and the raw materials are available; the santalum album L leaves contain high viterxin and isovitexin contents, thus a novel way for resource utilization of santalum album L leaves is provided; the two compounds can be prepared into small hydro-acupuncture drop injections, freeze-dried powder, tablets, capsules or pills; and the viterxin can be used for preparation of medicaments for treating coronary disease, myocardial infarction, angina and cancer prevention tumour resistance, and the isovitexin can be used for preparation of medicaments for treating or preventing prostate cancer, breast cancer and endometrial cancer.

Description

technical field [0001] The invention relates to the field of natural product extraction, in particular to a method for extracting and separating vitexin and isovitexin from natural products. Background technique [0002] sandalwood( Santalum album L ), also known as true sandalwood, white sandalwood, Zhentan, etc., is a semi-parasitic evergreen small tree of the genus Sandalwood in the family Sandalaceae. Native to southern India, Indonesia and other places, it is an important economic tree species integrating medicine, spices, Buddhist supplies, fine handicrafts and excellent carving materials. my country has a long history of using sandalwood. It is recorded in "Compendium of Materia Medica" by Li Shizhen in the Ming Dynasty: "Sandalwood; interpretation name: Zhantan, real sandalwood; smell: warm, pungent, non-toxic; indications: eliminating wind, heat, swelling, poison, and evil in the middle. Ghost qi, killing insects, relieving abdominal pain, cholera, kidney qi pain; ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D407/04
Inventor 贺丽苹黄娟娟彭国雄
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap