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Preparation method of fishbone polysaccharide of flatfish

A fish bone and polysaccharide technology, which is applied in the field of preparation of plaice fish bone polysaccharide, can solve the problems of slow enzymatic hydrolysis, low product yield, large alcohol consumption, etc., and achieves the effects of easy operation, high purity and short enzymatic hydrolysis time.

Inactive Publication Date: 2011-10-26
SHANDONG MEIJIA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method generally has the disadvantages of slow enzymatic hydrolysis, long reaction time, large amount of alcohol used for multiple dissolution and reprecipitation, low product yield, and dark color.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 500g of fresh plaice fish bones, remove the flesh from the fish bones, boil them in water at a temperature of 95°C for 60 minutes, fully remove the fat in the fish bones, and then clean and pulverize the fish bones.

[0023] Weigh 100g of crushed fresh plaice bone, add 200ml of water, add 200mmol of sodium hydroxide, stir intermittently for 300min, adjust the pH to 8.0 with dilute hydrochloric acid, add trypsin, papain and lipase in a mass ratio of 5:1:1 Composite hydrolase 50U / g fish bone, at 50°C, stir intermittently for 180min, adjust the pH value to 6.5, raise the temperature to 85°C, filter, add 15g activated carbon to the filtrate, stir intermittently for 60min at 60°C, filter, add to the filtrate The volume fraction is 95% alcohol, and stirring continuously, so that the volume content of alcohol in the solution is 70%, stop stirring, let stand until the polysaccharide is fully precipitated, then remove the upper layer of alcohol, dehydrate and dry the lower l...

Embodiment 2

[0025] Take 500g of fresh plaice fish bones, remove the flesh from the fish bones, boil them in water for 30 minutes at a temperature of 100°C, fully remove the fat in the fish bones, and then clean and pulverize the fish bones.

[0026] Weigh 100g of crushed fresh plaice bones, add 200ml of water, add 400mmol of potassium hydroxide, stir intermittently for 180min, adjust the pH to 9.0 with dilute hydrochloric acid, add trypsin, papain and lipase in a mass ratio of 5:2:10 Compound hydrolase 80U / g fish bone, at 48°C, stir intermittently for 300min, adjust the pH value to 8.0, raise the temperature to 90°C, filter, add 40g of activated carbon to the filtrate, stir intermittently at 100°C for 20min, filter, add to the filtrate The volume fraction is 95% alcohol, and stirring continuously, so that the volume content of alcohol in the solution is 60%, stop stirring, let stand until the polysaccharide is fully precipitated, then remove the upper alcohol, dehydrate and dry the lower p...

Embodiment 3

[0028] Take 500g of fresh plaice fish bones, remove the flesh from the fish bones, boil them in water at 100°C for 10 minutes, fully remove the fat in the fish bones, and then clean and pulverize the fish bones.

[0029] Weigh 100g of crushed fresh flatfish bones, add 1000ml of water, add 5000mmol of sodium hydroxide, stir intermittently for 30min, adjust the pH to 6 with dilute hydrochloric acid, add trypsin, papain and lipase in a mass ratio of 1:1:1 The compound hydrolase 10000U / g fish bone, at 60°C, stir intermittently for 180min, adjust the pH value to 5, raise the temperature to 85°C, filter, add 10g of activated carbon, stir intermittently for 30min at 100°C, filter, add volume fraction to the filtrate 95% alcohol, and constantly stirring, so that the volume content of alcohol in the solution is 70%, stop stirring, let stand until the polysaccharide is fully precipitated, then remove the upper layer of alcohol, dehydrate and dry the lower layer of polysaccharide to obtai...

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PUM

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Abstract

The invention discloses a preparation method of fishbone polysaccharide of flatfish. The method comprises the following steps: carrying out pre-treatment and base treatment on fishbone, and then carrying out enzymolysis treatment by using composite hydrolase of trypsinase, papain and lipase at the pH of 4-10 and the reaction temperature of 30-60 DEG C; and filtering, removing impurities and carrying out alcohol precipitation so as to obtain the fishbone polysaccharide of the flatfish. In the method, a mixed enzyme is used for the enzymolsis of the fishbone, enzymolsis time is short, and extraction rate is high; and activated carbon is used for impurity removal by absorption, and the impurities are thoroughly removed, thereby avoiding multi-time alcohol precipitation and saving alcohol amount and reducing production cost. The fishbone polysaccharide prepared by the method is light in color, high in purity and high in yield.

Description

technical field [0001] The invention relates to a preparation method of fish bone polysaccharide, in particular to a preparation method of plaice fish bone polysaccharide. Background technique [0002] Plaice, also known as flounder, inhabits the sandy bottom of shallow seas and preys on small fish and shrimps. It mainly lives in temperate waters and is an important economic fish in temperate waters. Because of their flat body, the eyes are on the upward side of the body, the color of this side matches well with the surrounding environment, and the downward side of their body is white. The flatfish has extremely fine scales on its body surface and only one dorsal fin, which extends from the head almost to the caudal fin. [0003] At present, there have been many literature reports on the preparation of polysaccharides from fish bones such as sharks and stingrays. According to research, the polysaccharides contained in flatfish bones have good health effects on the human bod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02
Inventor 张永勤彭英海申照华郭晓华安燕苑德顺
Owner SHANDONG MEIJIA GROUP
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