Bacillus pumilus, feed additive thereof, premix, and feed mildewproof agent

A technology of Bacillus pumilus and feed additive, which is applied in animal feed, animal feed, bacteria and other directions, can solve the problems that feed mildew inhibitor has not been proposed yet, and has little research, and achieves good mildew control effect, simple preparation method and palatability. strong effect

Active Publication Date: 2011-11-02
BEIJING DABEINONG TECH GRP CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, Bacillus pumilus is not a feed-grade microbial additive strain that can be directly fed to animals in China, and there are few studies on it.
[0005] At present, the application of Bacillus pumilus in China mainly focuses on its production of basic protein, lipopeptide and the prevention of plant diseases. There is no report on the application of Bacillus pumilus in animal feed, premix, and feed additives. The application of agents has not yet been proposed

Method used

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  • Bacillus pumilus, feed additive thereof, premix, and feed mildewproof agent
  • Bacillus pumilus, feed additive thereof, premix, and feed mildewproof agent
  • Bacillus pumilus, feed additive thereof, premix, and feed mildewproof agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Bacillus pumilus CGMCC No.4756

[0029] 1. Isolation of Bacillus pumilus CGMCC No.4756

[0030] (1) Take a small amount of rumen samples collected from Changping Cattle Farm cattle in Beijing, add sterile water to dilute to a certain concentration, boil in boiling water for 10 minutes, and then use LB medium to isolate Bacillus, and a total of 89 Bacillus strains were isolated.

[0031] (2) The 89 strains of bacillus isolated were subjected to an acid resistance test

[0032] The concentration of Bacillus pumilus cultivated in LB liquid medium for 12 hours was 1×10 8 cfu / mL bacterial suspension, add 1mL sterile normal saline solution with pH of 2.5, 3.0 and 3.5 respectively, incubate statically at 37°C for 4 hours, collect the bacteria and resuspend with 1mL sterile normal saline, and count viable bacteria by gradient dilution method , Measured its survival rate, and selected 11 strains of Bacillus tolerant to a pH value of 2....

Embodiment 2

[0044] Embodiment 2: preparation and application of antifungal agent

[0045] (1) Bacillus pumilus CGMCC No.4756, Bacillus subtilis and Bacillus licheniformis were aerobically cultured in LB liquid medium at 37°C for 12 hours, and the concentration of bacteria was adjusted to 1×10 8 , each bacterial solution was inoculated into unsterilized corn dregs according to the inoculum amount of 10% and fermented for 12 days; the sterile water with the same inoculum amount was inoculated into the fermented feed as a blank control.

[0046] The result shows that Bacillus pumilus CGMCC No.4756 and Bacillus subtilis have significant inhibitory effect on the growth of mold in the feed fermentation process, and the effect of Bacillus licheniformis is poor, and a large amount of molds ( Figure 4 -A)

[0047] (2) After continuing to ferment for 45 days, the same experimental effect as step 1 occurs ( Figure 4 -B).

[0048] (3) Bacillus pumilus CGMCC No.4756, Bacillus subtilis, and Bacill...

Embodiment 3

[0050] Embodiment 3: preparation and characteristic of feed additive

[0051] (1) Bacillus pumilus CGMCC No.4756, Bacillus subtilis and Bacillus licheniformis were aerobically cultured in LB liquid medium at 37°C for 12 hours, and the cell concentration was adjusted to 1×10 8 , inoculate the above-mentioned bacterium solution according to the inoculum amount of 5% in non-sterilized corn dregs to ferment for 3 days, and inoculate the fermented feed with the same inoculum amount in sterile water as a blank control. Take a certain amount of sample and dry it, dissolve it with sterile water, and carry out the colony counting analysis on the dilution plate.

[0052] The results showed that there was no significant difference in the number of viable bacteria between Bacillus pumilus CGMCC No.4756 and Bacillus subtilis and Bacillus licheniformis, reaching 1×10 10 -1×10 12 cfu / g, but the fragrance of fermented feed that does not exist in the control bottle, subtilis, and Bacillus li...

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Abstract

The invention belongs to the technical field of feed science and feed additives and particularly relates to Bacillus pumilus, a feed additive thereof, a premix, and a feed mildewproof agent. The Bacillus pumilus can be added into feed as a feed additive to improve the quality of the feed, increase crude protein content in feed, lower coarse fiber content in feed and is a new, safe, reliable, environment-friendly, purely-fermented and multifunctional microecological preparation product. The feed mildewproof agent, compared with the conventional mildewproof agent, has the advantages of safety, environment-friendliness, direct granulation, no chemical, high mildwproof effect, high antibacterial effect, capability of improving quality of feed, high palatability, small dosage and great conservation of production cost.

Description

technical field [0001] The invention belongs to the technical field of feed science and feed additives, and in particular relates to a bacillus pumilus, a feed additive, a premix and a feed antifungal agent thereof. Background technique [0002] Feed contamination by mold and mycotoxins is a problem that cannot be ignored in the current feed industry and animal husbandry production. According to the estimates of the United Nations Food Organization, about 5% to 7% of agricultural products such as grain and feed are damaged by molds in the world every year, which has caused huge economic losses. The feed contaminated by mold will decompose its nutrients, reduce its palatability, and produce a variety of mycotoxins. Animals will experience acute or chronic poisoning symptoms after eating moldy and deteriorated feed, resulting in slow growth of livestock and poultry. Decrease, lower digestibility, lower reproductive ability and immunity, seriously endanger animal health, and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/07A23K10/18
Inventor 吴慧玲刘滢王安如庞洁闫轶洁周娜刘鹏汪攀
Owner BEIJING DABEINONG TECH GRP CO LTD
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