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Method for preparing deionized formamide

A technology for deionizing formamide and formamide, which is used in the fields of forensic genetics and molecular biology, and can solve problems such as reducing flow and production capacity, and increasing fluid resistance.

Inactive Publication Date: 2011-11-16
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the resin particle size is less than 0.2mm (about 70 mesh), it will significantly increase the resistance of fluid passage, reducing the flow rate and production capacity

Method used

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  • Method for preparing deionized formamide
  • Method for preparing deionized formamide

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1, the preparation of deionized formamide

[0031] (1) Take 100 ml of analytically pure (99.9% by mass) formamide (purchased from Fisher Company) and put it in a flask.

[0032] Weigh out 25 g of Bio-Rad AG501-X8 resin with 5% crosslinking.

[0033] The resin is subjected to freeze-dehydration treatment, that is, freeze-dehydration is carried out overnight, so that the water in the ion exchange resin is completely evaporated to dryness, and the particle size of the above-mentioned resin is 300-1180um.

[0034] (2) Mix the freeze-dehydrated resin in the above step (1) with the formamide in the flask, and gently shake for no more than 7 hours. In this embodiment, shake for 3 hours to fully contact the ion exchange resin with formamide.

[0035] (3) Filter the homogeneously mixed mixed solution in the above step (2) through a pre-cleaned Whatman#1 filter (the pore size of the filter is 0.22um), and collect the filtrate to obtain deionized formamide.

[0036] (...

Embodiment 2

[0037] Embodiment 2, the preparation of deionized formamide

[0038] (1) Take 100ml of analytically pure (99.9%) formamide (purchased from Amresco), put it in a 250ml flask, and check its pH value.

[0039] Weigh out 5 g of Bio-Rad MB-3 resin with a degree of crosslinking of 5%.

[0040] The resin was added to a flask containing formamide (that is, the ratio of formamide: ion exchange resin was 100ml: 5g), and a magnetic stirring bar (length 1.2cm) was added.

[0041] (2) Cover the flask in which the magnetic stirrer has been added in the above step (1) and start the magnetic stirrer for stirring, the stirring speed is 600rpm.

[0042] According to the pH value of the formamide detected in the above-mentioned steps (1), determine the stirring time: 1. if the pH of initial formamide is less than 7.0, the magnetic stirring time is 45 minutes; 2. if the pH of initial formamide is greater than 7.0, the magnetic stirring time is 60 points. So far, a stirred mixed solution is obt...

Embodiment 3

[0045] Embodiment 3, detection analysis

[0046] 1. Determination of the water content of the prepared deionized formamide sample

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Abstract

The invention provides a method for preparing deionized formamide, which comprises the following steps: (1) formamide and ion exchange resin are mixed to obtain a mixture; (2) the mixture obtained from the step (1) is uniformly mixed, then the mixture is passed through a filter for removing ion exchange resin, collecting a filtrate to obtain the deionized formamide. The water content of deionized formamide prepared by the above scheme is below 1.8%, the conductivity of formamide is substantially lower than 100 muS / cm, the repeatability between batches is good and the stability under the room temperature is good, so that deionized formamide is capable of satisfying the application requirement. The detection results of capillary electrophoresis present that the internal standard peak type is sharp, the resolution is good, no non-specificity impurity peak generation, the sampling volume of DNA fragments is uniform, the distribution of peak height is normal, thereby the detection results present that the conductivity of deionized formamide conforms to the treating requirement of capillary electrophoresis samples.

Description

technical field [0001] The invention relates to the fields of forensic genetics and molecular biology. In particular, it relates to a preparation method of deionized formamide. Background technique [0002] Many molecular biology DNA detection techniques need to denature the sample DNA, even if the DNA molecule is loosened from a stable double helix structure to a single-stranded structure. All factors that can destroy the stability of the double helix can become denaturing conditions, such as heating, extreme pH, organic reagents such as methanol, ethanol, urea, and formamide, etc., can destroy the double helix structure and cause nucleic acid molecule denaturation. As a denaturant, formamide is widely used in nucleic acid hybridization and nucleic acid denaturation, such as Southern, Northern, Slot blot analysis, YAC screening, etc. Forensic DNA detection requires the use of formamide to denature sample DNA to maintain the DNA monotony The chain state is convenient for s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C233/03C07C231/24
Inventor 赵兴春叶健姜伯玮
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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