Detection method for water-soluble glucomannan
A glucomannan and detection method technology, which is applied in the field of water-soluble glucomannan detection, can solve the problems of lack of quality control methods for comprehensive evaluation of glucomannan products, and achieve strong operability and accurate results Effect
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Embodiment 1
[0041] The HPGPC-ELSD method described in this embodiment measures the molecular weight and the distribution range of the polysaccharides of Baiji:
[0042] Chromatographic conditions: Take about 10 mg of white and polysaccharide samples of various specifications, dissolve them in appropriate amount of water to prepare a concentration of 1.0 mg / mL, use TSK PW4000 type chromatographic column, GPC-ELSD analysis (Alltech Company) system, use water as mobile phase, flow rate 0.6mL / min, the column temperature of the chromatographic column is room temperature for analysis; the standard curve is prepared with a series of specifications of white and polysaccharide as reference substances, and the results of the samples to be tested are calculated by GPC software.
[0043] The absolute molecular weight of the reference substance BT01-07 was determined by the MALLS method. Get the solution of BT01-07, after filtering through a 0.22 μm microporous filter, adopt HPGPC-ELSD high-performanc...
Embodiment 2
[0047] The HPGPC-ELSD method described in the present embodiment measures the purity of polysaccharides from Baiji:
[0048] Chromatographic conditions: with embodiment 1;
[0049] Linear range: take a series of standard solutions of white and polysaccharide reference substance BT40, filter through a 0.22 μm microporous filter, inject samples for analysis, and record the peak area A of the chromatographic peak in the GPC spectrum of the solution. Linear regression was performed on lnm by lnA, and the regression equation of the white and polysaccharide reference substance BT40 was obtained: lnA=1.4924lnm+1.7227, R 2 =0.9984, it shows that the polysaccharide of Baiji has a good linearity between lnA and lnm in the concentration range of 0.5-5mg / mL. Calculated according to the signal-to-noise ratio S / N=3 and 10, the detection limit of HPGPC-ELSD analysis of polysaccharides in Baiji was 2.33 μg, and the limit of quantification was 5.48 μg.
[0050] Determination of the sample: t...
Embodiment 3
[0078] The HPLC-ELSD method described in this embodiment measures the monosaccharide composition and content of Baiji polysaccharide and konjac polysaccharide:
[0079] Preparation of sample solution: Precisely weigh 0.1806g of Baiji polysaccharide sample into a 25ml measuring bottle, dissolve with water and make to volume. Precisely measure 0.50 ml of the above solution into a sample bottle, add 125 μL of 10 mol / L trifluoroacetic acid solution, seal it with a cap, and perform hydrolysis reaction at 120 ° C for 2 h. After the reaction, blow dry at room temperature, add water to dissolve the residue, transfer to a 5mL volumetric flask, constant volume, and filter the solution through a 0.22μm microporous membrane for HPLC detection.
[0080] Preparation of the reference substance solution: Accurately weigh 0.01553 g of the reference substance mannose and 0.01662 g of anhydrous glucose into a 10 mL measuring bottle, dissolve them in water and make to volume as the reference subs...
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