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Optimized process for producing enramycin through fermenting streptomyces

A technology of Amellamycin and Streptomyces, which is applied in the fields of fermentation engineering, agricultural biotechnology, and industrial biotechnology to achieve the effects of increasing fermentation titer, huge economic and social benefits, and reducing costs

Inactive Publication Date: 2011-11-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Throughout the development at home and abroad, there is currently no report on the production of amycin by fermentation in China. Although Takeda Corporation of Japan has realized the industrial production of amycin, the highest output reported is only 4530mg / L

Method used

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  • Optimized process for producing enramycin through fermenting streptomyces
  • Optimized process for producing enramycin through fermenting streptomyces
  • Optimized process for producing enramycin through fermenting streptomyces

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The microorganism used was Streptomyces sp. NJWGY3665. Streptomyces was inoculated into the fermentation medium, and the concentration of each component in the fermentation medium was: glucose 35g / L, starch 20g / L, corn steep liquor 15g / L, yeast extract 1g / L, soybean cake powder 15g / L, Sodium chloride 5g / L, calcium carbonate 15g / L, ammonium chloride 5g / L, potassium dihydrogen phosphate 4g / L, ammonium sulfate 3g / L, MgSO 4 0.5g / L, cysteine ​​0.2mg / L; the method of feeding culture is used for cultivation, and the fermentation is carried out for 70 hours, and a glucose solution with a concentration of 60g / L is added to the fermentation system to make the carbon source concentration in the fermentation liquid Keep at 60g / L, when adding glucose solution, utilize calcium carbonate to adjust the pH, make the pH of the fermentation broth remain at 6.5, and the fermentation period is 8 days. The titer of Amramycin was 10621mg / L. Compared with the highest titer of 6630 mg / L in t...

Embodiment 2

[0036] The microorganism used was Streptomyces sp. NJWGY3665. Streptomyces was inoculated into the fermentation medium, and the concentration of each component in the fermentation medium was: glucose 25g / L, starch 5g / L, corn steep liquor 10g / L, yeast extract 5g / L, soybean cake powder 20g / L, Sodium chloride 8g / L, calcium carbonate 20g / L, ammonium chloride 8g / L, potassium dihydrogen phosphate 2g / L, ammonium sulfate 3g / L, CuSO 4 0.4g / L, arginine 0.4mg / L; the method of feeding culture is adopted for cultivation, and the fermentation is carried out for 78 hours, and a glucose solution with a concentration of 140g / L is added to the fermentation system to keep the concentration of carbon source in the fermentation liquid At 85g / L, when adding glucose solution, calcium carbonate was used to adjust the pH to keep the pH of the fermentation broth at 7.5, and the fermentation period was 6 days. The titer of Amramycin was 9217mg / L. Compared with the highest titer of 6630mg / L in the "Me...

Embodiment 3

[0039]

[0040]

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Abstract

The invention discloses an optimized process for producing enramycin through fermenting streptomyces. In the method, the formation of the enramycin is promoted through optimizing components of a culture medium, adding inorganic salt and low-molecular active substances (amino acid), disturbing the metabolic network of the streptomyces, and enhancing a formation approach of the target product; and the culture process is optimized through transforming the fermentation process and adopting a feed culture method. The optimized process for the fermentation of the enramycin, provided by the invention, is mainly used for greatly improving the fermentation potency of the enramycin and increasing the utilization rate of equipment and raw materials through optimizing the components of the culture medium and the feed process.

Description

technical field [0001] The invention relates to an optimized process for producing amellamycin by fermenting streptomyces, belonging to the fields of agricultural biotechnology, industrial biotechnology and fermentation engineering. technical background [0002] Feed antibiotics can promote the growth of livestock and poultry, prevent diseases, and greatly improve the economic benefits of animal husbandry. The livestock and poultry industries in many countries have long used antibiotic additives. However, the long-term, extensive and indiscriminate use of antibiotic feed has brought great trouble and difficulty to the control and treatment of bacterial diseases. Problems such as bacterial drug resistance and drug residues are becoming more and more prominent, which also pose potential threats to human health. At present, these situations have attracted the attention of governments and industry insiders all over the world. In 2006, the European Union completely banned the a...

Claims

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Application Information

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IPC IPC(8): C12P21/04C12R1/465
Inventor 胡永红杨文革万文倩
Owner NANJING UNIV OF TECH
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