Preparation technique of marine-organism-derived antihypertensive peptides

A marine biology and preparation process technology, applied in the biological field, can solve the problems of small production capacity, high energy consumption and high production cost, and achieve the effects of low cost, high separation efficiency and high depressurization activity

Active Publication Date: 2011-11-23
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a simple and safe process, low cost, high purity and low molecular weight for the problems of high production cost, small production capacity, and high energy consumption in the current preparation process of marine biological source antihypertensive peptides. , The preparation process of highly active marine biological source antihypertensive peptides, in order to make full use of marine biological by-products, turn waste into treasure, and develop high value-added marine biological source antihypertensive peptides

Method used

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  • Preparation technique of marine-organism-derived antihypertensive peptides
  • Preparation technique of marine-organism-derived antihypertensive peptides

Examples

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example 1

[0038] 1. Use high-quality fish scales as raw materials, and the selected fish scales can be marine fish scales or freshwater fish scales. Get 500kg of fish scales and drop into the stirring washer, add 5000kg of water, wash 3 times, and remove particle impurities. Put the washed fish scales into a cleaning machine, add 5000 kg of a mass volume concentration of 5% sodium bicarbonate solution, stir for 11 hours, and wash with water. Add 5000kg volume percent concentration and be 5% hydrochloric acid solution, stir for 8 hours, wash with water, remove sticky substance, fat and other impurities on the fish scale surface.

[0039] 2. Put the cleaned fish scales in the reaction kettle, add 5000kg of water, add 3kg of pepsin, adjust the pH value with sodium hydroxide and sulfuric acid, and control the pH of the reaction system to 1.5, enzymatic hydrolysis temperature 50 ℃, enzymatic hydrolysis After 6 hours, after the enzymatic hydrolysis reaction, the temperature was raised to 80°...

example 2

[0045] 1. Use high-quality fish scales as raw materials, and the selected fish scales can be marine fish scales or freshwater fish scales. Get 500kg of fish scales and drop into the stirring washer, add 5000kg of water, wash 3 times, and remove particle impurities. Put the washed fish scales into a cleaning machine, add 5000 kg of a mass volume concentration of 5% sodium bicarbonate solution, stir for 11 hours, and wash with water. Adding 5000kg volume percent concentration is 5% sulfuric acid solution, stirs 8 hours, washes with water, removes sticky matter, fat and other impurities on the fish scale surface.

[0046] 2. Put the washed fish scales in the reaction kettle, add 5000kg of water, add 1.5kg of pepsin, use sodium hydroxide and hydrochloric acid to adjust the pH value, and control the pH of the reaction system to 1, the enzymatic hydrolysis temperature is 40°C, and the enzyme After 6 hours of hydrolysis, after the end of the enzymatic hydrolysis reaction, the temper...

example 3

[0052] 1. Use high-quality fish scales as raw materials, and the selected fish scales can be marine fish scales or freshwater fish scales. Get 500kg of fish scales and drop into the stirring washer, add 5000kg of water, wash 3 times, and remove particle impurities. Put the washed fish scales into a cleaning machine, add 5000 kg of 1% sodium hydroxide solution, stir for 11 hours, and wash with water. Add 5000kg volume percent concentration and be 1% hydrochloric acid solution, stir 9 hours, wash with water, remove sticky substance, fat and other impurities on the fish scale surface.

[0053] 2. Put the cleaned fish scales in the reaction kettle, add 5000kg of water, add 4.5kg of acid protease, use sodium hydroxide and sulfuric acid to adjust the pH value, and control the pH of the reaction system to 2.5, the enzymatic hydrolysis temperature is 40°C, and the enzyme After 3 hours of hydrolysis, after the end of the enzymatic hydrolysis reaction, the temperature was raised to 80°...

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Abstract

The invention discloses a preparation technique of marine-organism-derived antihypertensive peptides, which comprises the following steps: (1) pretreating a marine organism byproduct to remove surface impurities and fat on the marine organism byproduct; (2) extracting marine-organism-derived proteins by an enzyme method; (3) separating and purifying the extracting solution by a membrane separation technique to obtain the marine-organism-derived proteins; (4) decomposing the marine-organism-derived proteins with protease, and deactivating the protease after the enzymolysis finishes, thereby obtaining a marine-organism-derived antihypertensive peptide solution; and (5) quickly drying the marine-organism-derived antihypertensive peptide solution by spray drying to obtain the finished marine-organism-derived antihypertensive peptide powder product of which the protein content is higher than 90%. By adopting the technique, the marine-organism-derived antihypertensive peptides have the advantages of low cost, high antihypertensive activity, low average relative molecular weight and high purity, fully utilizes the marine organism byproduct, changes wastes into valuables, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to a preparation process of a marine biological source hypotensive peptide, belonging to the field of biotechnology. Background technique [0002] Hypertension is a serious threat to human health. There are about 972 million hypertensive patients in the world, and 12 million people die from high blood pressure every year. The annual cost of treating high blood pressure and its complications is as high as trillions of dollars. , the efficacy of artificially synthesized antihypertensive drugs has been generally recognized by the medical community, but many side effects occur during clinical application, and high-safety antihypertensive peptides have become a research hotspot. The human body’s pressor system, the renial angiotensin system (RAS) and the blood pressure system, the kallikrein-kinin system (kallikrein-kinin system, KKS), are involved in blood pressure regulation and cardiovascular function play an important role. Angio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 陈俊德易瑞灶刘颖陈晖陈思谨张怡评孙继鹏许诺华
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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