Surface modification method of aliphatic polyester electrospun fiber by using sodium alginate and gelatin

Abstract

The invention provides a surface modification method of aliphatic polyester electrospun fiber by using sodium alginate and gelatin. The method comprises the following steps: dissolving aliphatic polyester to obtain aliphatic polyester spinning solution; electro-spinning the aliphatic polyester spinning solution to obtain aliphatic polyester superfine fibrous membrane; collecting and drying the aliphatic polyester superfine fibrous membrane; soaking the dried membrane in dilution solvent; soaking in sodium alginate solution; soaking in cross-linking agent aqueous solution at low temperature; and soaking in gelatin solution at low temperature. The surface modification method has the beneficial effects that the sodium alginate is fixed on the surface of the aliphatic polyester electrospun fiber by a physical method, and then the gelatin is covalently bonded with the sodium alginate by a chemical crosslinking method so as to obtain an aliphatic polyester electrospun fiber tissue engineering frame with double-layer natural macromolecular coatings, thus the obtained tissue engineering frame has the advantages of mechanical properties of aliphatic polyester as well as cellular affinity of natural macromolecules. The surface modification method is simple and convenient, and has wide application prospect in tissue engineering.

Description

technical field

[0001] The invention relates to a method for surface modification of an electrospinning superfine fiber membrane material. Background technique

[0002] Electrospinning is a simple and effective method for preparing ultrafine fibers. The fibers prepared by electrospinning are uniform in size, continuous and complete, and the aggregates composed of fibers have a large specific surface area, small and continuous pores, and high porosity. The extracellular matrix is ​​similar, so it plays a good role in promoting cell adhesion, growth and proliferation. Fiber aggregates with different structures can be obtained through different collection methods, which have different growth-guiding effects on cells, so they have attracted much attention in the field of tissue engineering.

[0003] The material composition of tissue engineering scaffolds is one of the key factors determining the interaction between cells and scaffolds. Typical representatives of synthetic degr...

Examples

Embodiment Construction

[0029] The following examples illustrate the present invention further.

[0030] PLGA was dissolved in hexafluoroisopropanol to obtain a concentration of 10% w / v spinning solution. Under the conditions of voltage 8kV, solution flow rate 0.5mL / h, and receiving distance 10cm, the electrospun PLGA fiber membrane was obtained by using a grounded metal plate, and dried in vacuum for 24h. Cut the fiber membrane to an appropriate size, immerse it in a diluted solvent of 10% v / v trifluoroethanol aqueous solution for 20 minutes, take it out and absorb the surface liquid, then immerse the fiber membrane in a 5mg / mL sodium alginate aqueous solution for 24 hours, take it out, rinse it with distilled water Absorb the surface moisture, soak the dried fiber membrane in 10mg / M1EDC / NHS (1:1) cross-linking agent aqueous solution at 4°C for 2h, take out the distilled water and rinse, soak the rinsed fiber membrane at 4°C at 10mg / mL In the gelatin solution, take it out after 24 hours, rinse with...

technical field

[0001] The invention relates to a method for surface modification of an electrospinning superfine fiber membrane material. Background technique

[0002] Electrospinning is a simple and effective method for preparing ultrafine fibers. The fibers prepared by electrospinning are uniform in size, continuous and complete, and the aggregates composed of fibers have a large specific surface area, small and continuous pores, and high porosity. The extracellular matrix is ​​similar, so it plays a good role in promoting cell adhesion, growth and proliferation. Fiber aggregates with different structures can be obtained through different collection methods, which have different growth-guiding effects on cells, so they have attracted much attention in the field of tissue engineering.

[0003] The material composition of tissue engineering scaffolds is one of the key factors determining the interaction between cells and scaffolds. Typical representatives of synthetic degr...

Examples

Embodiment Construction

[0029] The following examples illustrate the present invention further.

[0030] PLGA was dissolved in hexafluoroisopropanol to obtain a concentration of 10% w / v spinning solution. Under the conditions of voltage 8kV, solution flow rate 0.5mL / h, and receiving distance 10cm, the electrospun PLGA fiber membrane was obtained by using a grounded metal plate, and dried in vacuum for 24h. Cut the fiber membrane to an appropriate size, immerse it in a diluted solvent of 10% v / v trifluoroethanol aqueous solution for 20 minutes, take it out and absorb the surface liquid, then immerse the fiber membrane in a 5mg / mL sodium alginate aqueous solution for 24 hours, take it out, rinse it with distilled water Absorb the surface moisture, soak the dried fiber membrane in 10mg / M1EDC / NHS (1:1) cross-linking agent aqueous solution at 4°C for 2h, take out the distilled water and rinse, soak the rinsed fiber membrane at 4°C at 10mg / mL In the gelatin solution, take it out after 24 hours, rinse with...

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