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A skin external matrix for treating scars and its preparation method

A matrix and skin technology, applied in the field of external skin matrix, can solve the problem of inhibiting scar proliferation and other problems, and achieve the effects of maintaining the physiological balance of the skin, promoting the proliferation of epidermal cells, and improving the skin's moisturizing and defense capabilities.

Inactive Publication Date: 2011-12-07
上海美宝生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"However, it has not been found that it has the effect of inhibiting scar hyperplasia, and its medicinal use

Method used

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  • A skin external matrix for treating scars and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of cactus solid extract.

[0032] (1) The Mexican cactus (Opuntia Milpa Alta) was used in the experiment.

[0033] (2) The preparation process of cactus extract is as follows: a certain amount of fresh cactus is removed, washed and chopped, and then homogenized into a paste with a high-speed tissue grinder, and then 2 times of 95% ethanol is added to fully shake and stir. Extract for 3 hours, take the supernatant, and store it in the refrigerator; add an equal amount of ethanol to the residue, shake and stir fully, and extract for another 3 hours, take the supernatant and combine it with the first supernatant, suction filter, reduce Concentrate under high pressure, recover 1 ethanol, and freeze-dry the concentrated solution to obtain fresh cactus ethanol extract.

Embodiment 2

[0034] Example 2: Isolation and culture of fibroblasts in normal dermis and scar tissue.

[0035] (1) Digestion: Cut the normal skin discarded during surgery into pieces, digest with 0.1% collagenase at 37°C for 2 hours, filter through a 150-mesh stainless steel filter, centrifuge at 1000r / min for 5 minutes, and discard the supernatant , adding calcium and magnesium-free PBS to wash 3 times. Add 10ml of DMEM culture solution containing 10% fetal bovine serum, mix well, trypan blue staining and counting.

[0036] (2) Primary culture: after counting, make single cell suspension, press 1-2×106 / 75cm 2 Density inoculation culture, DMEM medium containing 10% fetal bovine serum, at 37°C, 5% CO 2 , cultured in an incubator with 100% relative humidity, and the culture medium was changed every 3 days.

[0037] (3) Subculture: When the cells are confluent to 60%-75% density, rinse with 10ml calcium and magnesium-free PBS, digest with 0.25% trypsin at 37°C for 5 minutes, add DMEM mediu...

Embodiment 3

[0039] Example 3: Effect of cactus extract on proliferation of dermal and scar fibroblasts.

[0040] a. experimental method:

[0041] (1) The third generation of dermal-derived fibroblasts (Dermal Fibroblast, DF) and scar-derived fibroblasts (Scar Fibroblast, SF) were selected for the experiment.

[0042] (2) 4000 cells per well were seeded into a 96-well plate at 37°C, 5% CO 2 , cultured in DMEM medium containing 10% fetal bovine serum for 12 hours.

[0043] (3) After 12 hours, replace the culture medium in each well, and add the culture medium containing cactus extract (DMEM containing 10% fetal bovine serum) again. The final concentrations of cactus extract in the culture medium are 10, 50, 200mg / mL. Continue incubation for 48 hours.

[0044] (4) After 48 hours, add 20ml MTS to each well and continue to incubate for another 2 hours.

[0045] (5) Measure the absorbance (Absorbance) of each well with an automatic microplate reader.

[0046] b. Experimental results:

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Abstract

The invention relates to an external skin base, in particular to a skin external base for treating scars and a preparation method thereof. The base is composed of 68.4% of the first mixture, 31% of the second mixture and 0.6% of guar gum according to weight percentage; wherein: the first mixture is composed of cactus juice 66.4% and triethanolamine 2%, and the second mixture is made of sesame oil 20%, stearic acid 8% and glyceryl monostearate 3%. The skin external matrix provided by the invention can inhibit scar fibroblast proliferation, inhibit and eliminate scar hyperplasia.

Description

technical field [0001] The invention relates to an external skin matrix, in particular to a skin external matrix for treating scars and a preparation method thereof, which are used for inhibiting fibroblast hyperplasia, inhibiting and eliminating scar hyperplasia. Background technique [0002] Wound healing refers to the healing process after the skin and other tissues are severed or damaged due to external forces. It is a complex combination of regeneration of various tissues, granulation tissue hyperplasia, and scar tissue formation. synergy of the process. Wound healing is essentially an inherent defensive adaptive response of the body to tissue cell damage caused by various harmful factors. [0003] The basis of wound healing is a series of biological activities of inflammatory cells such as macrophages, neutrophils, and repair cells such as fibroblasts and epidermal cells, and the cell matrix is ​​also involved. The wound healing process is mainly related to the depth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/33A61K47/36A61K47/44A61P17/02
Inventor 商庆新徐荣祥
Owner 上海美宝生命科技有限公司
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