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Simultaneous Quantitative Determination of Multiple Antibiotics at One Time

A technology for quantitative determination of antibiotics, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as sensitivity and detection limit, and achieve the effect of overcoming errors, saving time and efficient quantification, and simple steps

Inactive Publication Date: 2011-12-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thin-layer chromatography is used for rapid qualitative identification of drugs due to its simple and fast operation, but the sensitivity and detection limit of the method are poor compared to high-performance liquid chromatography

Method used

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  • Simultaneous Quantitative Determination of Multiple Antibiotics at One Time
  • Simultaneous Quantitative Determination of Multiple Antibiotics at One Time
  • Simultaneous Quantitative Determination of Multiple Antibiotics at One Time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Quantitative determination of antibiotics in stool samples

[0047] (1) Sample pretreatment

[0048] Take 3-5g of feces samples to cover the bottom of the petri dish, put it in a freeze dryer overnight and grind it to a particle size of 0.30mm, take 1g and extract it with 10ml of acetonitrile acidified with citric acid buffer to pH=4.7, and put it in a vortex machine Mix well, sonicate at 25°C for 15 minutes, centrifuge at 4000r / min for 15 minutes, repeat the extraction twice, collect the supernatant and filter it through a glass fiber membrane. The supernatant was extracted twice with 10 mL of n-hexane, and the extract was evaporated to near dryness with a rotary evaporator, diluted with double distilled water, and filtered through a glass fiber membrane. The extract passes through the PEP solid-phase extraction column activated by 5mL methanol and 5mL water in advance at a speed of 1 drop / s. 95 solution, and all the effluent was discarded. It was dried under reduce...

Embodiment 2

[0055] Quantitative determination of antibiotics in soil / sediment samples

[0056] (1) Sample pretreatment

[0057] Take 3-5g of the sample and spread it on the bottom of the culture dish, put it in a freeze dryer overnight and grind it to a particle size of 0.30mm, take 1g and add 20mL of EDTA-McIlvaine buffer solution, shake for 30min, take the upper layer extract, and extract repeatedly After 3 times, the 3 times of extracts were combined and filtered with a glass fiber membrane. The extract passes through the PEP solid-phase extraction column activated by 5mL methanol and 5mL water in advance at a speed of 1 drop / s. 95 solution, and all the effluent was discarded. It was pumped to dryness under reduced pressure for 3 min, and finally eluted with 8 mL of V (methanol): V (ethyl acetate) = 10:90 solution. The eluent was blown with nitrogen at 45°C, concentrated to dryness, and the standard solution obtained by mixing 0.4mL methanol and 0.6mL deionized water was used to dis...

Embodiment 3

[0064] Quantitative Determination of Antibiotics in Water Samples

[0065] (1) Sample pretreatment

[0066] The water sample is removed with a 0.45μm filter membrane to remove suspended matter, and 0.5mg Na is added to 1L of water sample 2 EDTA, and hydrochloric acid was added to adjust the pH to about 3.0. The extract passes through the PEP solid-phase extraction column activated by 5mL methanol and 5mL water in advance at a speed of 1 drop / s. 95 solution, and all the effluent was discarded. Dry it under reduced pressure for 5 min, and finally elute with 10 mL of V (methanol): V (ethyl acetate) = 10:90 solution. The eluate was blown with nitrogen at 40°C, concentrated to dryness, dissolved the residue with a standard solution obtained by mixing 0.4mL methanol and 0.6mL deionized water, and passed through a 0.45μm filter membrane to obtain a sample filtrate for liquid chromatography instrument measurement.

[0067] (2) HPLC-MS analysis

[0068] According to the ion chrom...

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Abstract

The present invention relates to a method for one-time simultaneous quantitative determination of multiple antibiotics, comprising: (1) preparing a sample filtrate; (2) setting liquid chromatography conditions and mass spectrometry conditions; (3) using the above liquid chromatography conditions and mass spectrometry conditions to determine For the sample filtrate prepared in step (1), analyze the data to determine the type and content of antibiotics in the analyzed sample. The present invention can simultaneously detect multiple antibiotics in one experiment and quantify the detected antibiotics at the same time. The combination of liquid chromatography-mass spectrometry not only solves the problem of difficult separation of peaks with similar peak times in liquid chromatography, but also overcomes the The error caused by the inconspicuous peak of low ion current intensity in the mass spectrum is eliminated; the invention can be widely used in the detection and quantitative analysis of antibiotics in various samples such as feces, soil, water, food, and the like.

Description

technical field [0001] The invention belongs to the field of detection of antibiotics, in particular to a method for one-time simultaneous quantitative determination of multiple antibiotics. Background technique [0002] Since the discovery of penicillin in 1928, antibiotics have been widely used in the treatment of human and animal diseases, and at the same time, sub-therapeutic doses have been added to animal feed for a long time to prevent diseases and promote animal growth. Antibiotics widely used at present can be divided into β-lactams, quinolones, tetracyclines, aminoglucosides, macrolides, polypeptides, etc. according to their chemical structures; according to their uses, they mainly include anesthetics and antacids , insect repellants, steroids, nonsteroids, anti-inflammatory drugs, antibacterial agents, fungicides, antiseptics, diuretics, emulsifiers, aphrodisiacs, sedatives and growth hormones, etc. Studies have shown that after ingestion of antibiotics, only a s...

Claims

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Application Information

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IPC IPC(8): G01N30/72
Inventor 冀秀玲刘芳
Owner DONGHUA UNIV
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