Simultaneous Quantitative Determination of Multiple Antibiotics at One Time

A technology for quantitative determination of antibiotics, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as sensitivity and detection limit, and achieve the effect of overcoming errors, saving time and efficient quantification, and simple steps
CN102269747AInactive Publication Date: 2011-12-07DONGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
DONGHUA UNIV
Publication Date
2011-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a method for one-time simultaneous quantitative determination of multiple antibiotics, comprising: (1) preparing a sample filtrate; (2) setting liquid chromatography conditions and mass spectrometry conditions; (3) using the above liquid chromatography conditions and mass spectrometry conditions to determine For the sample filtrate prepared in step (1), analyze the data to determine the type and content of antibiotics in the analyzed sample. The present invention can simultaneously detect multiple antibiotics in one experiment and quantify the detected antibiotics at the same time. The combination of liquid chromatography-mass spectrometry not only solves the problem of difficult separation of peaks with similar peak times in liquid chromatography, but also overcomes the The error caused by the inconspicuous peak of low ion current intensity in the mass spectrum is eliminated; the invention can be widely used in the detection and quantitative analysis of antibiotics in various samples such as feces, soil, water, food, and the like.
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Description

technical field

[0001] The invention belongs to the field of detection of antibiotics, in particular to a method for one-time simultaneous quantitative determination of multiple antibiotics. Background technique

[0002] Since the discovery of penicillin in 1928, antibiotics have been widely used in the treatment of human and animal diseases, and at the same time, sub-therapeutic doses have been added to animal feed for a long time to prevent diseases and promote animal growth. Antibiotics widely used at present can be divided into β-lactams, quinolones, tetracyclines, aminoglucosides, macrolides, polypeptides, etc. according to their chemical structures; according to their uses, they mainly include anesthetics and antacids , insect repellants, steroids, nonsteroids, anti-inflammatory drugs, antibacterial agents, fungicides, antiseptics, diuretics, emulsifiers, aphrodisiacs, sedatives and growth hormones, etc. Studies have shown that after ingestion of antibiotics, only a s...

Claims

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