Preparation method of water-insoluble corn dietary fiber with high oil-holding capacity

A water-insoluble, dietary fiber technology, applied in food preparation, food science, application, etc., can solve the problems of poor physical and chemical properties of water-insoluble dietary fiber, inability to fully exert physiological effects, etc., to improve the degree of comprehensive development and utilization, Low cost and good effect

Inactive Publication Date: 2013-06-12
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] On the other hand, the physical and chemical properties of water-insoluble dietary fiber recovered from corn husks are not good, especially the oil holding capacity is not as good as that of water-insoluble soybean dietary fiber isolated from soybean meal.
The typical test data obtained after measuring the oil holding power of multiple batches of corn fiber is that the oil holding power of corn bran is 2.02g / g, and its physiological efficacy cannot be fully exerted in the application

Method used

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  • Preparation method of water-insoluble corn dietary fiber with high oil-holding capacity
  • Preparation method of water-insoluble corn dietary fiber with high oil-holding capacity
  • Preparation method of water-insoluble corn dietary fiber with high oil-holding capacity

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Experimental program
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Effect test

Embodiment 1

[0038] Preparation of intermediate product water-insoluble corn fiber

[0039] First, the corn seed coats are screened, washed, dried, pulverized with a pulverizer, and passed through a 100-mesh sieve. The fat content of corn husk is only about 4%, and it can not be removed for lipophilic occasions.

[0040] Take the sieved corn bran powder and water in a mass ratio of 1:20 and make a slurry, adjust the pH to 7.5 with dilute alkali, add 1398 neutral protease, the enzyme dosage is 2000u / gDS, and enzymatically hydrolyze at 45°C 90min, then use dilute hydrochloric acid to adjust ph=6, add high-temperature-resistant a-amylase equivalent to 0.5% of dry corn bran powder, enzymolyze at 100°C for 60min, cool down to 60°C after the reaction, and then add equivalent Continuing to hydrolyze 0.3% glucoamylase of dry corn bran powder at 60°C for 120 minutes, heat to 90°C to inactivate the enzyme activity after the reaction is completed, filter the reverse enzymolysis solution after cool...

Embodiment 2

[0049] Take 100g of corn seed coat powder that has been screened, dried and crushed and passed through a 100-mesh sieve, add 1700mL of water to make a slurry, adjust the pH to 7.6 with dilute sodium hydroxide solution, add 300000u of 1398 neutral protease, and enzyme at 45°C Decompose for 85 minutes; adjust ph=5.6 with dilute hydrochloric acid, add 0.05g of high-temperature-resistant a-amylase, hydrolyze in boiling water bath for 85min, then cool down to 56°C, add 0.03g of glucoamylase, keep 56°C for 150min, and then heat to Incubate at 97°C for 15 minutes to inactivate the enzyme, cool down and filter, then wash and filter the obtained solid to obtain insoluble dietary fiber, then add it to 1300mL of distilled water, stir evenly, use dilute hydrochloric acid to pH 5.0, add 18mL of xylanase , control the temperature at 60°C, enzymolyze for 60 minutes, filter the enzymolyzate after cooling down, dry the obtained solid and grind it to obtain 89.5g of finished product, the measure...

Embodiment 3

[0051] Take 90g of corn seed coat powder that has been screened, dried, and crushed and passed through a 100-mesh sieve, add 1300mL of water to make a slurry, adjust the pH to 7.3 with dilute sodium hydroxide solution, add 300,000u of 1398 neutral protease, and enzyme at 46°C Decompose for 60 minutes, adjust ph=5.8 with dilute hydrochloric acid, add 0.05g of high temperature resistant a-amylase, hydrolyze in boiling water bath for 100min, then cool down to 56°C, add 0.02g of glucoamylase, keep 56°C for 180min, then heat to Incubate at 95°C for 20 minutes to inactivate the enzyme, cool down to 60°C and filter the enzymolysis solution, wash the obtained solid with water and suction filter, dissolve it in water to make a slurry and adjust the pH value to 5.5 with dilute hydrochloric acid, add 15mL of xylanase, and stir for 70 minutes for enzymolysis After cooling down, the reaction liquid was filtered by suction, the obtained solid was dried, and 82.1g of finished product was obta...

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Abstract

The invention provides a preparation method of water-insoluble corn dietary fiber with a high oil-holding capacity. The preparation method adopts a multi-enzyme successive enzymatic hydrolysis technology, wherein the multi-enzyme successive enzymatic hydrolysis technology is an enzymolysis technology adopting a neutral protease and a high-temperature resistant alpha-amylase and glucoamylase composite enzyme. The preparation method comprises the following steps of removing orderly impurities of starch, proteins and the like in corn husks to obtain purified water-insoluble corn fiber, and carrying out enzymolysis modification of the water-insoluble corn fiber by xylanase to obtain the water-insoluble corn dietary fiber with a high oil-holding capacity, wherein the oil-holding capacity is improved 81.7% from 2.02g / gDS to 3.67g / gDS and a oil-holding capacity of corn husks is 2.02g / gDS. The preparation method adopts a full enzymolysis technology. Through the preparation method, starch is removed; a water-insoluble fiber component in a product is retained; and subsequent neutralization treatment processes are avoided. In addition, the preparation method is utilized for processing and utilization of waste corn husks from starch and alcohol industries, and can reduce environmental pollution and improve a degree of comprehensive development and utilization of a by-product.

Description

technical field [0001] The invention relates to the production field of water-insoluble dietary fiber, in particular to a preparation method of high oil-holding power insoluble corn dietary fiber. Background technique [0002] Corn bran is a by-product produced in the production process of corn starch, and the quantity is very large. If it is not used rationally, it will not only waste resources, but also may cause environmental pollution. my country has very rich resources of corn husks, a by-product of corn processing. Using corn husks to prepare high-activity dietary fiber can reduce waste of resources, protect the environment, and improve the comprehensive development and utilization of by-products. [0003] The main components of corn bran are polysaccharides, hemicellulose, protein and water-insoluble fiber. It has high fiber content and is a source of dietary fiber with high quality and low price. The measured data of the main components of corn bran are shown in Tab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/105A23L1/308A23L7/104
Inventor 钟振声连小燕蔡佳振
Owner SOUTH CHINA UNIV OF TECH
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