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Pre-processing method for extracting tobacco leaf lignin by using enhanced enzymolysis method

A technology of lignin and enzymatic hydrolysis, applied in the field of lignin extraction, can solve the problems of time-consuming, low yield of structural change, long time-consuming, etc., and achieve the effects of improving enzymatic hydrolysis efficiency, avoiding dissolution loss, and simple and efficient operation.

Inactive Publication Date: 2015-05-27
HUZHOU PINCHUANG INCUBATOR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(3) Alkali pretreatment: Although most alkali pretreatments can improve enzymatic hydrolysis efficiency, there will still be problems with delignification. Ammonia expansion is a typical physical and chemical alkali pretreatment, but the hydrolysis rate is generally only 40% %~50%
Since the above pretreatment methods are all based on the purpose of increasing the conversion rate of sugar, but ignore the structural changes of lignin and the loss of lignin during the pretreatment process, resulting in low yields and other issues.
[0006] The pretreatment method used to extract tobacco leaf lignin by enzymatic hydrolysis mainly includes acetone extraction (Journal ofpulp and paper science, 2003,29(7):235-240), but it takes too long (48h); in addition, benzene / toluene - The mixed solvent of ethanol / ether is also commonly used in the pretreatment of lignin extraction (Industrial Crops Products.,2010,32:551-559)
The above organic solvent pretreatment method is not only time-consuming and dangerous, but also the pretreatment effect on tobacco leaves needs to be improved

Method used

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  • Pre-processing method for extracting tobacco leaf lignin by using enhanced enzymolysis method

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Experimental program
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Embodiment 1

[0025] Weigh 10g of tobacco leaf powder, and disperse the tobacco leaf powder in a mixed solution of 250ml of sodium hydroxide, urea and water at -10°C, the mass ratio of sodium hydroxide, urea and water in the mixed solution is 7:12:81 . After stirring for 30 minutes at -10°C, filter with a 600-mesh filter cloth, and keep the filtrate A for UV testing. The solid was washed with pure water to neutrality, and vacuum-dried at 50° C. for 24 hours to obtain 3.6738 g of pretreated tobacco leaf powder. The cellulose content of pretreated tobacco leaves was determined by the National Renewable Energy Laboratory analytical test method (NREL / TP-510-42618, Determination of Structural Carbohydrates and Lignin in Biomass.2008). Weigh 1g of the above-mentioned pretreated tobacco leaf powder, add it into 50mL acetic acid-sodium acetate buffer solution, the pH is 4.8, and the ionic strength is 50mmol / L, then add 20FPU / g dextran cellulase (CTec2, Novi Letter China Biotechnology Co., Ltd.), ...

Embodiment 2

[0029]Weigh 10g of tobacco leaf powder, and disperse the tobacco leaf powder in a mixed solution of 250ml of sodium hydroxide, urea and water at -10°C, the mass ratio of sodium hydroxide, urea and water in the mixed solution is 8:12:80 . After stirring for 30 minutes at -10°C, filter with a 600-mesh filter cloth, and keep the filtrate A for UV testing. The solid was washed with pure water to neutrality, and vacuum-dried at 50° C. for 24 hours to obtain 3.4737 g of pretreated tobacco leaf powder. The cellulose content of pretreated tobacco leaves was determined by the National Renewable Energy Laboratory analytical test method (NREL / TP-510-42618, Determination of Structural Carbohydrates and Lignin in Biomass.2008). Weigh 1g of the above-mentioned pretreated tobacco leaf powder, add it into 50mL acetic acid-sodium acetate buffer solution, the pH is 4.8, and the ionic strength is 50mmol / L, then add 20FPU / g dextran cellulase (CTec2, Novi Letter China Biotechnology Co., Ltd.), r...

Embodiment 3

[0031] Weigh 10g of tobacco leaf powder, and disperse the tobacco leaf powder in a mixed solution of 250ml of sodium hydroxide, urea and water at -10°C, the mass ratio of sodium hydroxide, urea and water in the mixed solution is 5:15:80 . After stirring for 30 minutes under the condition of maintaining -10°C, filter with a 600-mesh filter cloth, and keep the filtrate (named A) for ultraviolet testing. The solid was washed with pure water to neutrality, and vacuum-dried at 50° C. for 24 hours to obtain 3.7052 g of pretreated tobacco leaf powder. The cellulose content of pretreated tobacco leaves was determined by the National Renewable Energy Laboratory analytical test method (NREL / TP-510-42618, Determination of Structural Carbohydrates and Lignin in Biomass.2008). Weigh 1g of the above-mentioned pretreated tobacco leaf powder, add it into 50mL acetic acid-sodium acetate buffer solution, the pH is 5.0, and the ionic strength is 50mmol / L, then add 20FPU / g dextran cellulase (CTe...

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Abstract

The invention discloses a pre-processing method for extracting tobacco leaf lignin by using an enhanced enzymolysis method. The pre-processing method comprises the steps of dispersing dried tobacco leaf powder into a mixed aqueous solution of sodium hydroxide, urea and water, stirring, filtering, washing until filtrate is neutral, and drying to obtain pre-processed tobacco leaf powder. The invention further provides a method for extracting tobacco leaf lignin by using the enhanced enzymolysis method. By adopting the pre-processing method through sodium hydroxide, urea and a water solution, the disadvantages of lignin dissolution loss and change of a lignin structure due to the traditional pre-processing method can be overcome; the enzymolysis efficiency of cellulose in the tobacco leaf powder can also be increased; extraction and content measurement of lignin in subsequent raw materials can be carried out easily; a pre-processing reagent used in the invention is nontoxic, free from pollution and low in cost; and furthermore, the pre-processing method is simple and high-efficiency to operate.

Description

technical field [0001] The invention relates to the field of lignin extraction, more specifically, to a pretreatment method for extracting lignin from tobacco leaves by enzymolysis. Background technique [0002] With the continuous development of the tobacco industry, smoking and health issues have attracted much attention, and reducing tar and harm has become an inevitable trend in the development of the tobacco industry. Lignin in tobacco leaves is the main source of harmful substances such as polycyclic aromatic hydrocarbons and aromatic amines in tobacco tar. Studies have shown that the thermal cracking products of lignin mainly contain phenolic substances of methoxyl, hydroxyl and alkyl groups (Experimental Research on Rapid Pyrolysis of Lignin. 2005,39(5):710-714). During the smoking and burning process of cigarettes, the lignin in tobacco may be cracked to produce various phenolic substances with cancer-promoting effects. Studying the pyrolysis mechanism of tobacco ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H7/00
Inventor 史昕怡潘林武
Owner HUZHOU PINCHUANG INCUBATOR
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