M-4 strain for degradation of waste castor-based lubricating oil and mineral oil

Abstract

The invention relates to an M-4 strain for degradation of waste castor-based lubricating oil and mineral oil. The M-4 strain is characterized in that a category name of the M-4 strain is Alternaria sp.; a preservation number is CGMCC No. 4933; a preservation date is June 10, 2011; the preservation organization name is China general microbiological culture collection center; and the preservation address is No. 3, Court 1, Beichen West Road, Chaoyang District, Beijing City. Through a plate gradient coating separation method, a soil sample and a water sample are collected from a place around a sewage outlet of a castor oil squeezing workshop and a microorganism which grows through depending on castor-based lubricating oil as an only carbon source is screened out. The plate gradient coating separation method has simple, convenient and fast operation processes. The M-4 strain for degradation of castor-based lubricating oil can grow fast, is suitable for expansion re-culture under the conditions of an optimum culture temperature and a pH value and can be prepared into a strain starter to be dropped into a polluted environment for ecological restoration.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to an M-4 bacterial strain for degrading waste castor-based lubricating oil and mineral oil. Background technique [0002] With the rapid development of the global economy, a large amount of petroleum products are consumed every year. In addition to being used as refined oil such as gasoline and diesel, a large part is used as lubricating oil in different fields. During use, lubricating oil inevitably enters the environment through leakage, spillage or improper discharge. Since most of it cannot be biodegraded and has certain ecological toxicity, it seriously pollutes soil and water resources and damages the ecological environment. and ecological balance. According to statistics, about 5-10 million tons of petroleum-based chemicals enter the biosphere every year in the world, and 600,000 tons of lubricating oil enter the environment every year in the European Community alone. In our ...

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Problems solved by technology

In my country, the recovery rate of waste oil is less than 10%. According to this estimate, at least 280,000 to 320,000 tons enter the environment every year, causing a great waste of resources and environmental pollution.

Physical methods include excavation and landfill method, air stripping method, electrolysis method, washing method and isolation control method, etc. The heat treatment method is mainly used, that is, through incineration or calcination to purify most of the organic pollutants in the soil, but at the same time it also destroys the soil. structure and components, and the fuel and equipment used are expensive, so it is difficult to implement; chemical methods include oxidant oxidation, photochemical oxidation, thermal decomposition, extraction and chemical shed method, etc., and chemical leaching and washing can also be used to obtain Good degreasing effect, but the secondary pollution of the chemical reagents used limits its application

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