Microorganism flocculating agent and preparation method thereof

A microbial flocculant and flocculant technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of undisclosed purification methods of flocculant pure products, large amount of flocculant crude products, and undisclosed flocculants. Pure products and other problems, to achieve the effect of ideal flocculation effect, good promotion value and low production cost

Inactive Publication Date: 2012-01-11
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of culture fluid as a flocculant is easy to operate, it requires a large amount of fermentation culture fluid, so industrial application is very inconvenient and may cause secondary pollution
And another article "Research on the flocculation activity of biological flocculants produced by Galactomyces sp." (Earth and Environment, Volume 36, No. 3, 2008) discloses the research on the flocculation activity of biological flocculants produced by Galactomyces sp., but the study Only the crude product of the flocculant is disclosed in the literature, but the preparation conditions of the crude product have not been tested repeatedly. Pure product, also does not disclose the purification method of pure flocculant
Moreover, the amount of crude flocculant is large, and the flocculation effect is not as good as that of pure flocculant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Screening of Galactomyces geotrichum CGMCC 2535

[0035] Activated sludge from a sewage treatment plant (Beijing Qinghe Sewage Treatment Plant) was used as the source sample of the bacteria, and the medium was prepared according to the conventional method, and the sample was separated by the dilution coating plate separation method. After the formation of single colonies, press The solid culture medium used for each single colony was used to prepare a liquid culture medium, and the single colony was inoculated in the liquid medium according to a conventional method, and subjected to shaking culture in an air bath shaker to prepare a microbial culture medium. The cultured microorganisms were initially screened. The method of preliminary screening is: put 20ml of kaolin suspension with a concentration of 4g / L in the test tube, add 2-3 drops of the cultured microbial fermentation broth, and observe whether there are flocs formed. If flocs are formed, it means that the Bac...

Embodiment 2

[0037] 1. Preparation of flocculant

[0038] Including the following steps:

[0039] a) Determine the strain most suitable as microbial flocculant producing bacteria by screening from sludge;

[0040] The obtaining method of Galactomyces geotrichum CGMCC 2535 is the same as embodiment 1

[0041] b) Preparation of liquid culture medium for microbial flocculant producing bacteria;

[0042] The specific formula of the liquid medium is: based on 1000ml of distilled water, 18g of sucrose, FeSO 4 0.006g, K 2 PO 4 0.6g, (NH 4 ) 2 SO 4 0.6g, KCl 0.3g, NaNO 3 1.8g, MgSO 4 120.3g, pH natural. Preferably the pH is adjusted to pH 7.0.

[0043] C) fermentation culture of microbial flocculant producing bacteria;

[0044] Put the liquid culture medium into a 200ml seed culture container with a volume of 100ml, autoclave it, inoculate it with CGMCC 2535 strain after cooling, then put the seed culture device into an air bath constant temperature shaker, and heat it at 29°C-37°...

Embodiment 3

[0051] 1. Preparation of flocculant

[0052] a) to e) steps are the same as in Example 2.

[0053] 2. Flocculation effect test:

[0054] Add 0.3 ml of microbial flocculant liquid to 100 ml of blue-black ink diluted 50 times, mix well, and stand for a while to produce obvious flocculation After 120 minutes, the blue flocs precipitated, and the colorimetric decolorization rate reached 81.46%.

[0055] The method of producing flocculant by the bacteria is simple and easy; the suitable temperature of the flocculant is 10-80°C, the applicable pH is 3-9, and the dosage range is 0.1ml / L-1ml / L according to different treatment objects, and the dosage is small. The microbial flocculant produced by extraction has high flocculation efficiency, is non-toxic and harmless, has no secondary pollution in application, is biodegradable, and can be popularized and applied on a large scale. It has a wide range of applications, such as sewage treatment, including domestic sewage, industrial sewag...

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Abstract

The invention provides a preparation method of a microorganism flocculating agent by utilizing novel microorganism flocculating agent producing bacteria, i.e., Galactomyces geotrichum in CGMCC (China General Microbiological Culture Collection Center) NO.2535 and a microorganism flocculating agent prepared by using the method. The preparation method of the microorganism flocculating agent by utilizing the microorganism flocculating agent producing bacteria is simple and easy and is short in fermenting time and low in production cost; the produced flocculating agent has a wide applicable scope, a small using amount but high flocculating efficiency; and the microorganism flocculating agent which is prepared by extracting has no toxin or harm and no secondary pollution, is biodegradable and suitable for large-scale spread and application, and has a wide application prospect.

Description

technical field [0001] The invention relates to a microbial flocculant and a production method of the microbial flocculant. Background technique [0002] Flocculation technology is an economical and convenient treatment method to improve the efficiency of water treatment, and is widely used in industrial wastewater treatment, water supply treatment, food production and fermentation industries. In terms of water treatment, flocculation can effectively remove 80%-95% of suspended matter and 65%-95% of colloidal substances, which can effectively reduce the COD value in water; and through flocculation purification, usually more than 90% of microorganisms in water can be removed It is transferred to the sludge together with the virus, making it easy to disinfect and sterilize the water. The key to the flocculation effect depends on the choice of flocculant. [0003] As a substance that can coagulate and precipitate solid suspended particles that are not easy to settle in liquid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C02F1/52C12R1/01
Inventor 万鹰昕
Owner BEIJING UNION UNIVERSITY
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