Modified glassy carbon electrode (GCE) capable of simultaneously determining dopamine and uric acid in presence of ascorbic acid as well as preparation method and application thereof

A technology of glassy carbon electrode and ascorbic acid, which is applied in the field of electrochemical analysis and detection, can solve the problems of poor selectivity and achieve the effect of simple preparation method, easy operation and good selectivity

Inactive Publication Date: 2012-01-11
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Dopamine, uric acid, and ascorbic acid always exist in biological samples at the same time. The main problem in their detection is that the oxidation potentials of dopamine, uric acid, and ascorbic acid are almost the same as those of bare electrodes, and the selectivity is poor. Therefore, preparing various modified electrodes has become a Topics of current research

Method used

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  • Modified glassy carbon electrode (GCE) capable of simultaneously determining dopamine and uric acid in presence of ascorbic acid as well as preparation method and application thereof
  • Modified glassy carbon electrode (GCE) capable of simultaneously determining dopamine and uric acid in presence of ascorbic acid as well as preparation method and application thereof
  • Modified glassy carbon electrode (GCE) capable of simultaneously determining dopamine and uric acid in presence of ascorbic acid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of Pt-CoPc-MWNT Modified Glassy Carbon Electrode

[0026] (1) Ultrasonically mix 1g of phthalonitrile, 0.25g of anhydrous cobalt chloride, 1mL of DBU and 40mL of 0.0025g / ml multi-walled carbon nanotube suspension in n-pentanol, under nitrogen protection, 140°C Heating and stirring under low temperature for 2 hours, the product was filtered, washed with anhydrous methanol, and dried at 80°C for 4 hours to obtain dark black powder CoPc-MWNT;

[0027] (2) Weigh 0.02mg of multi-walled carbon nanotube-cobalt phthalocyanine into a round bottom flask, add 5mL of 1% chloroplatinic acid solution and 75mL of distilled water, stir and heat to 80°C, then add 15mL of 1% The sodium citrate solution was incubated for 1 h, then 2 mL of 1% sodium borohydride solution was added dropwise, stirred and refluxed for 4 h, the product was filtered, washed with absolute ethanol, and dried to obtain a black powder Pt-CoPc-MWNT;

[0028] (3) Weigh 6mg of Pt-CoPc-MWNT, and ultrasonica...

Embodiment 2

[0030] Preparation of Pt-CoPc-MWNT Modified Glassy Carbon Electrode

[0031] (1) Ultrasonically mix 0.6g phthalonitrile, 0.18g anhydrous cobalt chloride, 0.6mL DBU and 30mL 0.0033g / ml nitrobenzene suspension of multi-walled carbon nanotubes, heat and stir at 160°C After 3h, the product was filtered, washed with anhydrous methanol, and dried at 100°C for 2h to obtain dark black powder CoPc-MWNT;

[0032] (2) Weigh 0.04mg of CoPc-MWNT into a round bottom flask, add 10mL of 1% chloroplatinic acid solution and 40mL of distilled water, stir and heat to 80°C, then add 20mL of 1% sodium citrate solution dropwise to keep warm After 2 hours, 2 mL of 1% sodium borohydride solution was added dropwise, stirred and refluxed for 4 hours, the product was filtered, washed with absolute ethanol, and dried to obtain black powder Pt-CoPc-MWNT;

[0033] (3) Weigh 3 mg of Pt-CoPc-MWNT and ultrasonically disperse them in 1 mL of absolute ethanol; apply the uniformly dispersed composite modifier on...

Embodiment 3

[0035] Preparation of Pt-CoPc-MWNT Modified Glassy Carbon Electrode

[0036] (1) Mix 1.5g of phthalonitrile, 0.35g of anhydrous cobalt chloride, 2mL of DBU and 60mL of 0.003g / ml trichlorobenzene suspension of multi-walled carbon nanotubes by ultrasonic, heat and stir at 160°C for 2h ,, the product was filtered, washed with anhydrous methanol, and dried at 100°C for 2 hours to obtain dark black powder CoPc-MWNT;

[0037] (2) Weigh 0.08mg of CoPc-MWNT into a round bottom flask, add 20mL of 1% chloroplatinic acid solution and 75mL of distilled water, stir and heat to 80°C, then add 30mL of 1% sodium citrate solution dropwise to keep warm 1h, then add 2mL of 1% sodium borohydride solution dropwise, stir and reflux for 4h, filter the product, wash with absolute ethanol, and dry to obtain black powder Pt-CoPc-MWNT;

[0038](3) Weigh 3 mg of Pt-CoPc-MWNT and ultrasonically disperse them in 1 mL of absolute ethanol; apply the uniformly dispersed composite modifier on the surface of a...

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Abstract

The invention relates to a modified glassy carbon electrode (GCE) capable of simultaneously determining dopamine and uric acid in the presence of ascorbic acid as well as a preparation method and an application thereof, and belongs to the technical field of electrochemical analysis and detection. The modified GCE is modified by platinum nanoparticles-cobalt phthalocyanine-multi-walled carbon nanotubes (MWCNTs), wherein, the cobalt phthalocyanine is formed on the MWCNTs in situ, and the platinum nanoparticles are formed on the cobalt phthalocyanine in situ. The preparation method comprises the following steps: preparing a cobalt phthalocyanine-MWCNTs composite material by an in-situ synthesis method; then adsorbing the platinum nanoparticles onto the surface of the cobalt phthalocyanine-MWCNTs by a chemical reduction method; and finally obtaining the GCE modified by the platinum nanoparticles-cobalt phthalocyanine-MWCNTs by a dispensing method. The modified GCE prepared by the method is taken as a novel electrochemical biological sensor and can simultaneously detect the dopamine and the uric acid in the presence of the ascorbic acid. The preparation method is simple, fast and easy to operate and good in selectivity.

Description

technical field [0001] The invention relates to a glassy carbon electrode for simultaneously detecting dopamine and uric acid in the presence of ascorbic acid, a preparation method and application thereof, and belongs to the technical field of electrochemical analysis and detection. Background technique [0002] Dopamine is an important neurotransmitter in the central nervous system, and low concentrations of dopamine may lead to neurological disorders such as Parkinson's syndrome and schizophrenia. Uric acid is an end product of urine metabolism, and high or low concentrations of uric acid are symptoms of many diseases, such as gout and hyperuricemia. Ascorbic acid is known for its antioxidant properties, and numerous studies have shown that ascorbic acid plays an important role in gene expression and as a co-substrate for dioxygenases. Dopamine, uric acid, and ascorbic acid always exist in biological samples at the same time. The main problem in their detection is that th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 左霞李楠卜媛
Owner CAPITAL NORMAL UNIVERSITY
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