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Method for rapidly detecting synthetic phenol antioxidant in edible oil and fat and oil and fat food

A technology for phenolic antioxidants and oils and fats food, which is applied in the direction of material analysis by observing the influence on chemical indicators, and analysis by chemical reaction of materials, etc. , complicated operation and other problems, to achieve the effect of low cost, broad market application prospects and good reproducibility

Inactive Publication Date: 2012-01-18
云南健牛环境监测有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to literature reports, the most commonly used methods for analyzing and detecting BHA, TBHQ, and PG content are gas chromatography, high performance liquid chromatography, gas chromatography-mass spectrometry, and electrochemical methods. Instrument, high cost, time-consuming, not suitable for on-site testing
[0005] In the determination of propyl gallate (PG) in GBT5009.32-2003 oils and fats, although the colorimetric method is also used, compared with the method of the present invention, this method consumes a lot of organic solvents and is cumbersome to operate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The rapid detection of PG in first-grade rapeseed oil, the experimental process includes the following steps: (1) Take 5 mL of first-grade rapeseed oil in a clean separatory funnel; (2) Add 1 mL of anhydrous methanol and 1 mL of acetonitrile to the separatory funnel ; (3) Shake, stand and separate layers, take out the supernatant, extract twice, and combine the supernatant; (4) Add 1g / L, 2mL 3-methyl-2-benzothiazolone hydrazone to the supernatant Hydrochloride methanol solution and 1g / L, 1mL cerium ammonium sulfate aqueous solution; (5) Shake well at room temperature, let it stand for 3 minutes, and measure it at a wavelength of 403nm by a spectrophotometer. The first-grade rapeseed oil sample contains PG0. 018g / kg.

Embodiment 2

[0025] The rapid detection of BHA in lard, the experimental process includes the following steps: (1) Take 2g of lard in a clean Erlenmeyer flask, add 2mL of absolute ethanol, 2mL of absolute methanol, and 1mL of acetonitrile into the Erlenmeyer flask; (2) After shaking for 7 minutes, filter the extract; (3) add 1.5g / L, 2mL 3-methyl-2-benzothiazoline hydrazone hydrochloride methanol solution and 1g / L, 2.5mL ammonium cerium sulfate to the filtrate in sequence Aqueous solution; (4) Shake well at room temperature, let it stand for 5 minutes, and measure it with a spectrophotometer at a wavelength of 494nm. The lard sample contains 0.026g / kg of BHA.

Embodiment 3

[0027] The rapid detection of TBHQ in biscuits, the experimental process includes the following steps: (1) Take 2g of biscuits, crush them and put them in a clean Erlenmeyer flask, add 3mL absolute ethanol and 2mL acetone to the Erlenmeyer flask; (2) Shake for 12min Finally, filter the extract; (3) add 0.5g / L, 2mL 3-methyl-2-benzothiazolone hydrazone hydrazone hydrochloride methanol solution and 2g / L, 2mL cerium ammonium sulfate aqueous solution to the filtrate successively; (4 ) Shake well at room temperature, let it stand for 7 minutes, and measure it with a spectrophotometer at a wavelength of 464nm. The sample in the biscuit contains 0.015g / kg of TBHQ.

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Abstract

The invention discloses a method for rapidly detecting the content of a synthetic phenol antioxidant, particularly the content of BHA (Butyl Hydroxy Anisol), TBHQ (Tert-Butyl Hydroquinone) and PG (Propyl Gallate), in edible oil and fat and oil and fat food. The method comprises the following steps of: by using an organic solvent as an extracting agent, uniformly mixing the extracting agent and a sample to be detected; carrying out oscillating extraction; taking extraction liquid; adding 3-methyl-2-benzothiazolinonehydrazone hydrochloride and ammonium ceric sulfate which are used as color development reagents into the extraction liquid; shaking the mixture well; standing; and rapidly detecting the content of the synthetic phenol antioxidant by a spectrophotometry. In the method, large-scale expensive equipment such as an HPLC (High Performance Liquid Chromatography), a GC (Gas Chromatograph) and the like are not required. An instrument required by the method is simple. The method is simple and convenient to operate, can be completed by non professional staffs, has low cost, high accuracy and reproducibility, is time-saving and labor-saving and has wide market application prospect.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and specifically relates to a rapid detection of synthetic phenolic antioxidants in edible oils and oily foods, especially butyl hydroxyanisole (BHA), tertiary butyl hydroquinone (TBHQ ) and propyl gallate (PG) content. Background technique [0002] Edible oils and oily foods are indispensable consumer goods in daily life, and are important foods that provide heat energy and essential fatty acids to the human body and promote the absorption of fat-soluble vitamins. In order to avoid small molecule aldehydes, ketones and other substances produced by rancidity of edible oils and oily foods due to the influence of various factors (air, light, temperature, moisture, impurities, heavy metal ions and microorganisms, etc.) during long-term storage, Harm people's health, often add synthetic antioxidants, such as PG, TBHQ, BHA, BHT and so on. [0003] Butylated hydroxyanisole (BHA), tert...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 杨亚玲陈邈张涛
Owner 云南健牛环境监测有限公司
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