Capillary tube electrophoresis electrochemiluminescence detecting method for galantamine hydrobromide in traditional Chinese medicinal short-tube lycoris extract

A galantamine and luminescence detection technology, applied in the field of capillary electrophoresis electrochemiluminescence detection, can solve the problems of complex operation and expensive high-performance liquid chromatography-mass spectrometer equipment, and achieve high photon yield, high selectivity, good The effect of anti-interference ability

Inactive Publication Date: 2014-01-15
CHANGCHUN INST OF TECH +1
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Problems solved by technology

However, the high-performance liquid chromatography-mass spectrometer equipment is expensive, the operation is complicated, and the use of organic reagents constitutes a technical material obstacle that is difficult to promote the use of this technology.

Method used

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  • Capillary tube electrophoresis electrochemiluminescence detecting method for galantamine hydrobromide in traditional Chinese medicinal short-tube lycoris extract

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Embodiment 1

[0050] Example 1 The steps and conditions of the capillary electrophoresis electrochemiluminescence detection method for galantamine in the Chinese medicine Lycoris extract are as follows:

[0051] The steps and conditions of the capillary electrophoresis electrochemiluminescence detection method for galantamine in the extract of Chinese medicine Amaryllis lycoris are as follows:

[0052] 1.1 Preparation of the sample to be inspected

[0053] 1.1.1 Preparation of galantamine standard solution

[0054] The galantamine standard substance was dissolved in secondary water to prepare a galantamine standard substance stock solution, the concentration of the stock solution was 5.0 mmol / L, and the stock solution was stored in a refrigerator at 4°C;

[0055] Dilute the galantamine standard stock solution with secondary water to a concentration of 2.0×10 -5 The galantamine standard solution of mol / L;

[0056] 1.1.2 Preparation of related solutions of lycoris extract

[0057] A. Prep...

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Abstract

The invention provides a capillary tube electrophoresis electrochemiluminescence detecting method for galantamine hydrobromide in a traditional Chinese medicinal short-tube lycoris extract. In the method, the detection limit of the galantamine hydrobromide is 1.0*10<-9> mol / L, being 1-2 orders of magnitude lower than that of a common mass spectrometric detection method; the linear range is 2.5*10<-7>-5.0*10<-5> mol / L, overcrossing two orders of magnitude; the sensitivity of the galantamine hydrobromide is detected by using pyridine-ruthenium electrochemiluminescence; efficient separation of the galantamine hydrobromide is realized with a phosphate running buffer solution; the sample size is upgraded by using sodium, and derivation of a sample to be detected is not required; and the traditional Chinese medicinal short-tube lycoris extract solution is directly used for detecting after being filtered and diluted without complex procedures such as drying, purifying and the like. In the invention, a capillary tube electrophoresis electrochemiluminescence detecting technology is applied to the detection of the galantamine hydrobromide in the traditional Chinese medicinal short-tube lycoris extract for the first time.

Description

technical field [0001] The invention belongs to the technical field of capillary electrophoresis electrochemiluminescence detection, in particular to a method for capillary electrophoresis electrochemiluminescence detection of galantamine in a traditional Chinese medicine Lycoris extract. Background technique [0002] The integration of traditional Chinese medicine and advanced detection technology can play a positive role in improving the competitiveness of Chinese medicine in the international market and expanding the share of Chinese medicine in the international market. Lycoris is a traditional Chinese medicine. The alkaloids in Lycoris have important medicinal value. For example, galantamine in Lycoris can be used to prepare medicines for treating Parkinson's and other diseases. People have been researching and exploring new methods for the detection of active components in Lycoris. The efficient separation and sensitive detection of galantamine in Lycoris extract is an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/88G01N27/447G01N21/76A61P25/16A61K125/00
Inventor 高英向前孟祥萍龙北生高嬿马戈唐娟韩冰雁
Owner CHANGCHUN INST OF TECH
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