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Triple-helical Tremellan, preparation method and application thereof

A tremella polysaccharide and triple helix technology, applied in the field of polysaccharides, can solve problems that do not involve the identification of activity or advanced structure

Inactive Publication Date: 2012-02-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the above reports are about the crude extraction method of polysaccharides. The analysis of single component structure in some patents or patent applications is about the primary structure of tremella polysaccharides, and does not involve the identification of activity or advanced structure.

Method used

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  • Triple-helical Tremellan, preparation method and application thereof
  • Triple-helical Tremellan, preparation method and application thereof
  • Triple-helical Tremellan, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Water extraction: clean the fruiting body of Tremella fungus with tap water, dry it in an oven at 50°C to constant weight, crush it to obtain fruiting body powder of Tremella fungus, add distilled water, add 50mL of distilled water per gram of fruiting body of Tremella fungus, mix well and put in Extract in a water bath at a constant temperature at 96°C for 4 hours, centrifuge at 4000 rpm at 4°C for 20 minutes, and take the supernatant; repeat the centrifuged precipitate twice according to the water extraction method, and combine the supernatant to obtain the tremella polysaccharide water extract;

[0048] (2) Alcohol precipitation: add 4 times the volume of the tremella polysaccharide water extract to the tremella polysaccharide water extract in step (1), and the volume percentage concentration is 95% ethanol aqueous solution, stir and mix, place in a 4 ° C refrigerator Precipitate overnight, then centrifuge at 4°C 4000rpm for 20min, take the precipitate obtained by...

Embodiment 2

[0055] Embodiment 2: Component detection

[0056] With the tremella polysaccharide TFP1 that embodiment 1 makes, it is 81.9% to detect TFP1 total sugar content with phenol-sulfuric acid method, detect without albumen with Coomassie Brilliant Blue method (Bradford method), detect uronic acid content with m-hydroxyphenol method to be 81.9%. 6.8%. 1mg / ml TFP1 aqueous solution was scanned by 200-400nm UV, the results are shown in image 3 , at 260nm and 280nm, there is no obvious absorption peak, indicating that the sample does not contain nucleic acid and protein.

Embodiment 3

[0057] Example 3: Monosaccharide composition

[0058] Tremella polysaccharide TFP15mg prepared in Example 1 was added to 2ml of 2mol / L sulfuric acid, placed in a stoppered test tube, sealed with nitrogen, hydrolyzed at 100°C for 12h, cooled to room temperature, neutralized with barium sulfate, centrifuged, and the supernatant was freeze-dried to obtain Freeze-dried powder (i.e. tremella polysaccharide TFP1 hydrolyzed sample), to be derivatized. Dissolve various monosaccharides and uronic acid standard substances in 0.3M (mol / L) sodium hydroxide aqueous solution to prepare the monosaccharide and uronic acid standards that each monosaccharide and uronic acid concentration is 5mmol / L (mM) solution, the Tremella polysaccharide TFP1 hydrolyzed sample was dissolved in 0.3M aqueous sodium hydroxide solution to prepare the TFP1 solution with a tremella polysaccharide TFP1 hydrolyzed sample concentration of 5mmol / L, and then 75ul of monosaccharide and uronic acid standard solution and ...

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Abstract

The invention discloses triple-helical Tremellan, a preparation method and application thereof. The preparation method comprises the following steps of: extracting Tremellan components through water extraction and alcohol precipitation, removing protein by using an enzyme-Sevage combined method, performing dialysis, and purifying by using anionexchange chromatography and gel filtration chromatography, and performing vacuum freeze drying, separation and purification on the components to obtain the Tremellan. By the method for preparing the Tremellan, the natural structure and activity of the Tremellan are not influenced; and the method has low requirement on equipment and low cost, and is suitable for large-scale promotion, development and use in industrial production. The purified Tremellan is subjected to component analysis, structure identification and antioxidant activity research, and results show that the Tremellan has high activity of removing hydroxyl radicals and is a potential antioxidant substance.

Description

technical field [0001] The invention relates to the field of polysaccharides, in particular to a triple helix white fungus polysaccharide and its preparation method and application. Background technique [0002] Tremella fuciformis Berk, also known as tremella fuciformis Berk, contains carbohydrates, proteins, vitamins and various amino acids, etc., and is a good medicine for nourishing and strengthening the body. Tremella fruiting body consists of several to more than 10 thin and wrinkled petals, in the shape of chrysanthemum, peony or hydrangea, 3-15cm in diameter, white or yellowish, smooth and shiny surface, kitty yellowish brown Horny, hard and brittle; swollen when soaked in water, gelatinous, slightly smelly, light in taste, soft when fresh, shrinking after drying; mostly grown on the rot of oak and other broad-leaved trees. According to pharmacological studies at home and abroad, tremella polysaccharide can regulate the intestinal beneficial flora of animals to enha...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12S3/02A61K31/715A61P39/06
Inventor 李卫旗贺亮马素云韦朝阳
Owner ZHEJIANG UNIV
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