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Chemiluminescent immunoassay kit for detecting mycoplasma pneumoniae IgM antibody

A technology for chemiluminescence immunity and mycoplasma pneumoniae, which is applied in the direction of chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, and measurement devices, to achieve the effects of easy storage, small differences between product batches, and reliable diagnostic basis

Active Publication Date: 2012-03-07
同昕生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no kit with high sensitivity and good stability for the detection of Mycoplasma pneumoniae based on chemiluminescence immunoassay

Method used

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  • Chemiluminescent immunoassay kit for detecting mycoplasma pneumoniae IgM antibody
  • Chemiluminescent immunoassay kit for detecting mycoplasma pneumoniae IgM antibody
  • Chemiluminescent immunoassay kit for detecting mycoplasma pneumoniae IgM antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, kit preparation method of the present invention

[0022] 1, the test kit of the present invention comprises:

[0023] 1) Mycoplasma pneumoniae IgM positive control; 2) Mycoplasma pneumoniae IgM negative control; 3) Mycoplasma pneumoniae IgM calibrator; 4) Microwell plate coated with FITC monoclonal antibody; 5) FITC-labeled Mycoplasma pneumoniae antigen; 6) Anti-human IgM Enzyme label; 7) 20-fold concentrated washing solution; and 8) chemiluminescence substrate solution.

[0024] Wherein, the microwell plate is a microwell strip with 24, 48 or 96 holes; the anti-human IgM is goat anti-human IgM polyclonal antibody, rabbit anti-human IgM polyclonal antibody or mouse anti-human IgM monoclonal antibody; the enzyme horseradish peroxidase.

[0025] 2, the preparation method of No. I kit of the present invention:

[0026] The positive control was prepared with the positive serum of Mycoplasma pneumoniae IgM, the negative control was prepared with the serum of...

Embodiment 2

[0071] Embodiment 2. Comparative test of the luminescence substrate of the kit of the present invention and the commercially available chemiluminescence substrate solution

[0072] Control kit: the same as the kit in Example 1, the difference is that the chemiluminescence substrate is a commercially available conventional chemiluminescence substrate solution (purchased from Huzhou Yingchuang Biotechnology Co., Ltd.).

[0073] Step 1. The detection samples are 254 cases of respiratory infection samples and blood samples of healthy people in Example 1.

[0074] The detection operation steps are the same as those in Step 3 of Example 1. The results are shown in the table below.

[0075] Table 4. No. I kit chemiluminescent substrate of the present invention is compared with the chemiluminescent substrate sold in the market

[0076]

[0077] The data described in Table 4 shows that the positive coincidence rate of the No. I kit chemiluminescent substrate of the present inventi...

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PUM

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Abstract

The invention provides a chemiluminescent immunoassay kit for detecting a mycoplasma pneumoniae IgM antibody, which belongs to a medical detection reagent. The chemiluminescent immunoassay kit comprises a monoclonal antibody coated microplate of anti fluorescein isothiocyanate, fluorescein isothiocyanate labeled mycoplasma pneumoniae antigen, horseradish peroxidase labeled anti-human IgM and a chemiluminescent substrate. The method has the advantages of low background, high specificity and strong affinity, simultaneously, effectively reduces the batch difference and has large application value in the immunodetection field.

Description

technical field [0001] The invention relates to a medical detection reagent, in particular to a chemiluminescent immunoassay kit for detecting the IgM antibody of mycoplasma pneumoniae. Background technique [0002] Mycoplasma pneumoniae (Mycoplasma pneumonia, MP) was first isolated from the sputum of patients with atypical pneumonia in 1962. It is a prokaryotic cell that is between bacteria and viruses, has no cell wall, and can grow and reproduce on non-living media. Organisms belonging to the phylum Protozoa, Mollicula, Mycoplasma, Mycoplasma, and Mycoplasma genus are important agents that cause children's tracheitis, bronchitis, primary atypical pneumonia, and community acquired pneumonia (CAP). Pathogens, infection can also cause complications such as encephalitis, myocarditis, immune hemolytic anemia, nervous system damage, kidney damage, infectious mononucleosis, and Kawasaki disease. [0003] MP is transmitted through droplets in the form of aerosol particles, which...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/543G01N33/533G01N21/76
Inventor 彭京胜焦守恕李全
Owner 同昕生物技术(北京)有限公司