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Method for extracting active proteins with antioxidant and hypoglycemic functions from mushrooms

A technology for Lentinus edodes protein and water-soluble protein, which is applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc., can solve the problems of unsatisfactory protein yield and activity, and achieve the effect of simple preparation process and high yield.

Active Publication Date: 2013-03-06
江苏荣诚农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the direct extraction method of alkali method is usually used to separate and extract the protein from mushrooms, but the yield and activity of the protein are not very satisfactory, and the application of a large amount of industrial alcohol has become an obstacle to the application of mushroom protein in the food field

Method used

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  • Method for extracting active proteins with antioxidant and hypoglycemic functions from mushrooms
  • Method for extracting active proteins with antioxidant and hypoglycemic functions from mushrooms
  • Method for extracting active proteins with antioxidant and hypoglycemic functions from mushrooms

Examples

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Effect test

Embodiment 1

[0025] The method for extracting the active protein with antioxidant and hypoglycemic effects from shiitake mushrooms is characterized in that it comprises the following steps:

[0026] (1) pulverizing shiitake mushrooms;

[0027] (2) Water extraction of shiitake mushroom protein: adding 1 mass part of crushed shiitake mushroom powder to 15 mass parts of deionized water, extracting at 4°C for 2 hours, filtering under reduced pressure to obtain water-soluble protein extract of shiitake mushroom; and drying filter residue;

[0028] (3) Salt solution extraction of shiitake mushroom protein: the filter residue obtained in step (2) is added to 0.2M NaCl aqueous solution, the ratio of the shiitake mushroom to the NaCl aqueous solution is 1g: 15ml, extracted at 4°C for 2 hours, and depressurized Filtrating to obtain the salt-soluble protein extract of mushrooms; and drying the filter residue;

[0029] (4) Alkaline solution extraction of shiitake mushroom protein: add the filter res...

Embodiment 2

[0036] The method for extracting the active protein with antioxidant and hypoglycemic effects from shiitake mushrooms is characterized in that it comprises the following steps:

[0037] (1) pulverizing shiitake mushrooms;

[0038] (2) Water extraction of shiitake mushroom protein: add 1 mass part of shiitake mushroom powder crushed into 15 mass parts of deionized water, extract at 5°C for 2 hours, and filter under reduced pressure to obtain water-soluble protein extract of shiitake mushroom; and dry the filter residue ;

[0039] (3) Salt solution extraction of shiitake mushroom protein: the filter residue obtained in step (2) is added to 0.1M NaCl aqueous solution, the ratio of the shiitake mushroom to the NaCl aqueous solution is 1g: 15ml, extracted at 5°C for 2 hours, and depressurized Filtrating to obtain the salt-soluble protein extract of mushrooms; and drying the filter residue;

[0040] (4) Alkaline solution extraction of shiitake mushroom protein: add the filter resi...

Embodiment 3

[0047] The method for extracting the active protein with antioxidant and hypoglycemic effects from shiitake mushrooms is characterized in that it comprises the following steps:

[0048] (1) pulverizing shiitake mushrooms;

[0049] (2) Water extraction of shiitake mushroom protein: add 1 mass part of crushed shiitake mushroom powder to 20 mass parts of deionized water, extract at 1°C for 3 hours, filter under reduced pressure to obtain water-soluble protein extract of shiitake mushroom; and dry the filter residue ;

[0050] (3) Salt solution extraction of shiitake mushroom protein: the filter residue obtained in step (2) is added to 0.2M NaCl aqueous solution, the ratio of the shiitake mushroom to the NaCl aqueous solution is 1g: 20ml, extracted at 1°C for 3 hours, and depressurized Filtrating to obtain the salt-soluble protein extract of mushrooms; and drying the filter residue;

[0051] (4) Alkaline solution extraction of shiitake mushroom protein: add the filter residue ob...

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Abstract

The invention discloses a method for extracting active proteins with antioxidant and hypoglycemic functions from mushrooms, comprising the following steps of: (1) smashing the mushrooms; (2) extracting mushroom proteins by utilizing water; (3) extracting the mushroom proteins by utilizing saline solution; (4) extracting the mushroom proteins by utilizing alkaline solution; (5) extracting the mushroom proteins by utilizing alcoholic solution; (6) carrying out vacuum concentration; (7) salting out; (8) dialyzing; and (9) freeze drying: respectively freeze drying the dialyzed solution and respectively obtaining mushroom water-soluble proteins, mushroom salt-soluble proteins, mushroom alkali-soluble proteins and mushroom alcohol-soluble proteins. Compared with a conventional extraction method directly using alkali, the method disclosed by the invention has the advantages that: protein content of a sample is obviously increased; due to the fractionation effect of different solvents, the proteins with higher activity are enriched and the showed antioxidant and hypoglycemic activities are obviously enhanced; and the preparation process is simple, convenient, non-toxic, safe and high in yield.

Description

technical field [0001] The invention relates to a method for extracting active protein from shiitake mushrooms, belonging to the technical field of natural active ingredient extraction. Background technique [0002] Shiitake is a fungus that grows on wood and is known as the "Queen of Plants" due to its rich nutrition. Lentinus edodes is sweet, flat and cool in nature and flavor; it enters the liver and stomach meridians. It has the effects of nourishing the liver and kidney, strengthening the spleen and stomach, nourishing qi and blood, improving intelligence and calming the nerves, and beautifying the face. It can also reduce phlegm and regulate qi, benefit the stomach and harmonize the middle, detoxify, anti-tumor, and treat acne. Indications for loss of appetite, physical weakness, urinary incontinence, constipation, obesity, tumor sores and other diseases. [0003] According to reports, in addition to water, fresh shiitake mushrooms contain crude protein, crude fat, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/375C07K1/36C07K1/34C07K1/30
Inventor 陈海霞章钰张宁马利帅
Owner 江苏荣诚农业科技发展有限公司