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Anti-bacterial peptide hydrolysate of earthworm and feed comprising the same

A peptide hydrolyzate and earthworm technology, applied in the field of earthworm antibacterial peptide hydrolyzate, can solve the problems of reducing antibacterial peptide antibacterial activity, digestive enzyme activity, complicated production process, and affecting the use effect, so as to achieve improved disease resistance, simple production process, Effect of Feed-to-Meat Ratio Reduction

Active Publication Date: 2012-04-11
JIANGMEN HAOLUN ECOLOGICAL AGRI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention has a certain antibacterial and antibacterial effect, but the production process is relatively complicated, and the production cost per unit potency is high
Secondly, the form of the product is a solid feed material, and the adsorption function of the vermicompost absorbs the special taste of the vermicompost, which affects its food-attractiveness
Thirdly, although it can be used in the production of feed mills, the pelleting and post-aging processes in the production process of preparing feed will reduce the antibacterial activity of antimicrobial peptides and the activity of digestive enzymes, thereby affecting its use effect

Method used

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  • Anti-bacterial peptide hydrolysate of earthworm and feed comprising the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Prepare the earthworm antimicrobial peptide hydrolyzate according to the following specific steps:

[0024] 1. Collect fresh earthworms, put the earthworms mixed with vermicompost in the sun, because earthworms are afraid of light, they will drill into the bottom layer, scrape off the surface layer of vermicompost, and then continue to drill down, and then scrape the surface layer of vermicompost until the earthworms form a ball , only a small amount of vermicompost remains;

[0025] 2. Soak the fresh earthworms obtained in step 1 in water, stir, wash 3 to 5 times until clean, and filter out the water;

[0026] 3. The earthworms, brown sugar, water, and sorbic acid that have been filtered dry are hydrolyzed for 20 hours in a water bath at a constant temperature of 40° C. according to a weight ratio of 1:0.2:1:0.007;

[0027] 4. Filter the impurities to obtain the earthworm antimicrobial peptide hydrolyzate.

Embodiment 2

[0029] Prepare the earthworm antimicrobial peptide hydrolyzate according to the following specific steps:

[0030] 1. Collect fresh earthworms, put the earthworms mixed with vermicompost in the sun, because earthworms are afraid of light, they will drill into the bottom layer, scrape off the surface layer of vermicompost, and then continue to drill down, and then scrape the surface layer of vermicompost until the earthworms form a ball , only a small amount of vermicompost remains;

[0031] 2. Soak the fresh earthworms obtained in step 1 in water, stir, wash 3 to 5 times until clean, and filter out the water;

[0032] 3. Expose the drained earthworms in the sun to make dried earthworms;

[0033] 4. The dried earthworms, brown sugar, water, and potassium sorbate were hydrolyzed for 16 hours in a water bath at a constant temperature of 60° C. according to a weight ratio of 1: 1.1: 10: 0.036;

[0034] 5. Filter the impurities to obtain the earthworm antimicrobial peptide hydrol...

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Abstract

The invention provides an anti-bacterial peptide hydrolysate of earthworm. The method for preparing the anti-bacterial peptide hydrolysate comprises the following steps: 1) collecting fresh earthworm, cleaning and drying; 2) hydrolyzing the earthworm after being processed in the step 1), brown sugar, water and preservative through water bath at constant temperature of 40-60 degrees centigrade for16-20 hours according to weight rate of 1: 0.2-1.1: 1-10:0.007-0.003; 3) filtering the impurities to obtain the anti-bacterial peptide hydrolysate of the earthworm. The anti-bacterial peptide hydrolysate of the earthworm provided by the invention comprises more than ten amino acids, digestive enzymes and small peptides which are suitable for aquatic animals. The anti-bacterial peptide hydrolysateof the earthworm is the mixture of multiple anti-bacterial peptides of the earthworm, multiple digestive enzymes and amino acids thereof, cooperates the pathogenic bacteria, has broad-spectrum antibacterial property, improves the feed utilization rate, reduces the feed / meat ratio, improves the immunity of the organism of the aquatic animals, promotes the growth of the aquatic animals, improves the quality of the aquatic animals, has simple production technology, and reduces the production cost.

Description

technical field [0001] The invention belongs to the field of animal food additives, in particular to an earthworm antibacterial peptide hydrolyzate. Background technique [0002] With the continuous development of the aquaculture industry, aquaculture failure is often caused by diseases and stress, which brings great economic losses to producers. Antibiotics are more and more widely used in aquaculture, which can prevent and control diseases, promote growth, and save nutrients. The application of antibiotics has effectively controlled the occurrence of many aquatic diseases, such as fish gill rot, enteritis, saprolegniasis, Chinese velvet sting edema, prawn limb rot, etc., which has greatly promoted the development of the aquaculture industry. develop. However, since the use of antibiotics is prone to produce drug-resistant strains, there are drug residues and the micro-ecological balance of aquatic animals is easy to destroy, making the prevention and control of aquacultu...

Claims

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Application Information

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IPC IPC(8): A23K1/10
Inventor 冯耀民黄惠花黄国伦
Owner JIANGMEN HAOLUN ECOLOGICAL AGRI CO LTD
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