Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of gene sequences related to syndrome of dampness-heat of liver and gallbladder of chronic hepatitis B (CHB) in traditional Chinese medicine

A chronic hepatitis B, gene sequence technology, applied in the application field of gene sequence, can solve the problem of no chronic hepatitis B and so on

Inactive Publication Date: 2012-04-18
SHANGHAI BIOCHIP +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no report on the gene sequence for the auxiliary diagnosis of chronic hepatitis B TCM liver and gallbladder damp-heat syndrome

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of gene sequences related to syndrome of dampness-heat of liver and gallbladder of chronic hepatitis B (CHB) in traditional Chinese medicine
  • Application of gene sequences related to syndrome of dampness-heat of liver and gallbladder of chronic hepatitis B (CHB) in traditional Chinese medicine
  • Application of gene sequences related to syndrome of dampness-heat of liver and gallbladder of chronic hepatitis B (CHB) in traditional Chinese medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 clinical sample

[0035] (a) Sample preparation for model establishment: 6 chronic hepatitis B patients with TCM liver and gallbladder damp-heat syndrome and 6 chronic hepatitis B patients without TCM liver and gallbladder damp-heat syndrome were fasted in the morning (9-10 a.m.) 3ml of peripheral venous blood was extracted and stored at -20°C in a blood RNA collection tube from Applied Biosystems. This step was completed with the assistance of the Department of Liver Diseases and Laboratory of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.

[0036] (b) Sample preparation for model validation: Collect 35 chronic hepatitis B peripheral venous blood samples for expanded validation, and the processing method is the same as above.

Embodiment 2

[0037] Example 2 Extraction, purification and quality detection of whole blood leukocyte total RNA

[0038] (1) Extraction and purification of total RNA from whole blood leukocytes

[0039] The whole blood RNA extraction kit (Tempus Spin RNA Isolation Reagent Kit) from Applied Biosystems was used to extract and purify the total RNA of leukocytes, and the operation steps were carried out according to the standard operating instructions of the kit.

[0040] (2) Quality detection of total RNA

[0041] The extracted RNA was analyzed with an Agilent 2100bioanalyzer. Clear peak shapes and minimal background fluorescence indicated intact, undegraded RNA (see figure 1 ).

Embodiment 3

[0042] Example 3 Gene expression profile experiment

[0043] (1) Chip experiment process

[0044] The Affymetrix U-219 expression profile chip was used, and the experimental process was carried out in strict accordance with the Affymetrix expression profile chip operation manual.

[0045] (2) Chip scanning and data reading

[0046] chip adopts Scanner 3000 (P / N00-00212, Affymetrix) was used to scan, and CommandConsole Software 3.1 (Affymetrix) was used to read the original data.

[0047] (3) Data standardization

[0048] The quality control qualified data was normalized by Gene Spring Software 11.0 (Agilent), and the algorithm used was MAS5.0.

[0049] (4) Difference screening and statistical analysis

[0050] Differential gene screening, based on the detection signal in 80% of the samples, Gene Spring Software 11.0 (Agilent) was used for the T-test combination of differential genes between groups of chronic hepatitis B with TCM liver and gallbladder damp-heat syndrome a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of a group of gene sequences related to syndrome of dampness-heat of liver and gallbladder of chronic hepatitis B (CHB) in traditional Chinese medicine in preparation of a product for aided diagnosis of the syndrome of dampness-heat of liver and gallbladder of CHB in traditional Chinese medicine. By adopting the group of the gene sequences, the syndrome of the dampness-heat of liver and gallbladder of CHB in traditional Chinese medicine can be accurately differentiated according to a molecular level, and the gene sequences can be taken as a drug target for treating the syndrome of the dampness-heat of liver and gallbladder of CHB in traditional Chinese medicine.

Description

technical field [0001] The invention relates to the field of molecular diagnosis, in particular to the application of a group of gene sequences related to chronic hepatitis B traditional Chinese medicine liver and gallbladder damp-heat syndrome. Background technique [0002] Patients with chronic hepatitis B (chronic hepatitis type B, CHB) refer to those who have a history of hepatitis B virus or HBV carrier in the past, or have acute hepatitis B for more than half a year, but still have symptoms, signs, and abnormal liver function. my country is an area with a high incidence of chronic hepatitis B. According to statistics, 750 million people have been infected with HBV (accounting for 57.6% of the national population), of which about 120 million are chronically infected with HBV, and 15% to 25% of chronically infected people will eventually die For liver diseases associated with HBV, such as cirrhosis and liver cancer. About 300,000 cases of chronic hepatitis B or related d...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68G01N33/53A61K45/00A61P1/16
Inventor 张庆华彭景华王世东胡义扬赵瑜王珂魏婷
Owner SHANGHAI BIOCHIP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com