Primers and probe for detecting fragment S of rift valley fever virus

A Rift Valley fever virus and probe technology, applied in the field of genetic engineering, can solve problems such as differences in pathogenicity of virus strains, and achieve the effects of high sensitivity, high sensitivity, and rapid response
CN102433392AInactive Publication Date: 2012-05-02CHINESE ACAD OF INSPECTION & QUARANTINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINESE ACAD OF INSPECTION & QUARANTINE
Publication Date
2012-05-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides primers and a probe for detecting the fragment S of a rift valley fever virus, wherein, a forward primer is 5'-GGATTACTTTCCTGTGATATCTGTTG-3'; a reverse primer is 5'-GTATCCTGGGAGGRCCATCWC-3', R refers to A or G and w refers to T or A); and a probe is 5'-F1-ACTCCACTGACACAACACGACGACCACT-Q1-3', F1 is a fluorescent reporter and Q1 is a fluorescent quencher. The invention also provides a real-time fluorescence RT-PCR (reverse transcription-polymerase chain reaction) detection method for the fragment S of the rift valley fever virus. In the method, pseudovirus grains of the rift valley fever virus are taken as a template, and the primers and the probe are used to carry out real-time fluorescent RT-PCR, thus judging whether the detected virus is the rift valley fever virus according to the detected result. The probe provided by the invention has high sensitivity and good specificity, and has no cross reaction with all of Africa swine fever virus, irides virus and goatpox virus.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to a primer and a probe for detecting the S segment of Rift Valley fever virus. Background technique

[0002] Rift valley fever (Rift valley fever, RVF) is a serious zoonotic disease and an endemic disease that has been prevalent in the Grand Canyon of East Africa since ancient times. It is mainly transmitted among sheep, goats and cattle, and can often cause abortion in pregnant animals. Storm (abortion storm), young sheep mortality rate as high as 90%. The epidemic cycle of the disease is about 5-10 years. The disease can infect humans, and the patients show flu-like symptoms such as fever, headache and arthralgia. A few have hemorrhagic fever and encephalitis, and occasionally can cause retinitis and even blindness. The case fatality rate is about 1%. Up to about 50%. RVF is mainly transmitted by mosquitoes, has obvious seasonality, and can also be infected by contact. [0...

Claims

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