Method for pathologically fixing experimental animal lung

Abstract

The invention discloses a method for pathologically fixing an experimental animal lung, which comprises the following steps of: (1) filling a fixing liquid, namely, suspending a filling container filled with the fixing liquid onto an isolated animal lung, inserting the liquid outlet end of a filling tube connected with the filling container into trachea of the isolated animal lung, and filling the fixing liquid into pulmonary alveoli of the animal lung by utilizing the hydrostatic pressure formed by the height difference between the liquid surface of the fixing liquid and the horizontal surface of the animal lung so as to naturally extend the animal lung; and (2) soaking the fixing liquid, namely, completely soaking the animal lung obtained in the step (1) into the fixing liquid to be fixed. The method is convenient to operate, low in cost and fast in fixing; the method can be controlled in a normalizing manner; and the method can enable the animal lung to be completely, uniformly and naturally filled, so that natural shapes of lung tissues is favorably stored and the need of observing pathological change of small blood vessels and small tracheas of the lung is met.

Description

technical field [0001] The invention relates to a pathological fixation method for experimental animal lungs, which belongs to the field of biotechnology. Background technique [0002] Animal experiments are one of the most important and commonly used scientific research methods in current scientific research, and are the basic research methods of various disciplines such as pharmacology, physiology, and pathophysiology. Respiratory diseases (such as COPD, pulmonary vascular disease, lung cancer, etc.) are major diseases that endanger human health. Therefore, research on respiratory diseases is an important research content for many medical researchers. Animal models of respiratory diseases have been widely used in the pathogenesis of respiratory diseases Mechanism research and new drug development, in which the pathological results of experimental animals are important indicators for judging the success of the model and scientific research. Standardized pathological proces...

AI Technical Summary

Problems solved by technology

At present, formalin or 4% paraformaldehyde is often used for pathological fixation of the lungs to directly soak and fix the isolated animal lungs. This fixation method causes the lung volume to shrink sharply, compresses the alveoli and vascular lumen, and affects the peripheral bronchi and Accurate judgment of vascular pathological changes

In addition, some researchers use a syringe to inject fixative into the lungs of animals through the trachea, but this method often destroys the original tissue shape of the animal lungs due to excessive pressure, or affects the experimental results due to uneven filling of the lungs

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