Method for pathologically fixing experimental animal lung

A technology of experimental animals and immobilization methods, applied in the preservation of human or animal body, preparation of test samples, animal husbandry, etc., can solve the problems of uneven lung filling, alveolar compression, affecting experimental results, etc., to facilitate standardized operation , fast fixation, good repeatability

Inactive Publication Date: 2012-05-02
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, formalin or 4% paraformaldehyde is often used for pathological fixation of the lungs to directly soak and fix the isolated animal lungs. This fixation method causes the lung volume to shrink sharply, compresses the alveoli and vascular lumen, and affects the peripheral bronchi and Accurate judgment o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for pathologically fixing experimental animal lung
  • Method for pathologically fixing experimental animal lung
  • Method for pathologically fixing experimental animal lung

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Utilize the present invention to fix rat lung tissue and carry out pathological section to observe lung tissue morphology

[0019] Materials and methods:

[0020] 1. Main experimental materials

[0021] 1. SPF grade SD rats: weight 200g-250g, Guangdong Experimental Animal Center

[0022] 2. Sodium pentobarbital: Guangzhou Chemical Second Factory

[0023] 3. Paraformaldehyde: Sigma

[0024] 4. Hematoxylin: Guangzhou Chemical Reagent No. 2 Factory

[0025] 5. Yihong: Second Guangzhou Chemical Reagent Factory

[0026] 2. Main instruments

[0027] 1. Citadel 1000 tissue dehydrator: British Shandon company

[0028] 2. BM-Ⅱ water bath biological tissue embedding machine: Shenzhen Xin'an Yida Electronics Factory

[0029] 3. Optical microscope: Japan Olympus Company

[0030] 4. Integrated digital photography system: Nikon Corporation of Japan

[0031] 5. Fluorescence inverted microscope: Leica, Germany

[0032] 3. Experimental method

[0033] ...

Embodiment 2

[0051] The difference from Example 1 is that the isolated lungs of mice are adopted, the fixative is 0.6ml of 4% (mass percentage concentration) formaldehyde solution, the height between the liquid level of 4% formaldehyde solution and the level where the mouse lungs are located The distance is 6cm. The whole lungs of the mice were completely immersed in 4% formaldehyde solution for 48 h.

[0052] test results :from Figure 2A and Figure 2B As shown, after HE staining of mouse lung tissue fixed by the present invention, the tissue structure is complete and clear, the structure of the alveolar cavity is clear, the gas-blood barrier structure at the end can be seen, the small artery and small vein at the end of the lung have a clear structure, and a single layer of endothelial cells can be seen and smooth muscle cell layer; after HE staining of the mouse lung tissue fixed by the existing common method, the structure of the end of the lung is clearer, the alveoli are shrunk, t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for pathologically fixing an experimental animal lung, which comprises the following steps of: (1) filling a fixing liquid, namely, suspending a filling container filled with the fixing liquid onto an isolated animal lung, inserting the liquid outlet end of a filling tube connected with the filling container into trachea of the isolated animal lung, and filling the fixing liquid into pulmonary alveoli of the animal lung by utilizing the hydrostatic pressure formed by the height difference between the liquid surface of the fixing liquid and the horizontal surface of the animal lung so as to naturally extend the animal lung; and (2) soaking the fixing liquid, namely, completely soaking the animal lung obtained in the step (1) into the fixing liquid to be fixed. The method is convenient to operate, low in cost and fast in fixing; the method can be controlled in a normalizing manner; and the method can enable the animal lung to be completely, uniformly and naturally filled, so that natural shapes of lung tissues is favorably stored and the need of observing pathological change of small blood vessels and small tracheas of the lung is met.

Description

technical field [0001] The invention relates to a pathological fixation method for experimental animal lungs, which belongs to the field of biotechnology. Background technique [0002] Animal experiments are one of the most important and commonly used scientific research methods in current scientific research, and are the basic research methods of various disciplines such as pharmacology, physiology, and pathophysiology. Respiratory diseases (such as COPD, pulmonary vascular disease, lung cancer, etc.) are major diseases that endanger human health. Therefore, research on respiratory diseases is an important research content for many medical researchers. Animal models of respiratory diseases have been widely used in the pathogenesis of respiratory diseases Mechanism research and new drug development, in which the pathological results of experimental animals are important indicators for judging the success of the model and scientific research. Standardized pathological proces...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28A01N1/00
Inventor 卢文菊王健陈豫钦江倩云昕林纯意
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap