Lung knock injury animal model construction method and application thereof

A lung blast injury and animal model technology, applied in the field of medicine, can solve the problems of damage data gap, inability to simulate the complexity and destructiveness of shock waves, and unreported research on resveratrol, so as to improve the activity without producing Effects of antibiotic contamination, susceptibility to resistance

Active Publication Date: 2021-11-12
兵器工业卫生研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most lung blast injury models are mainly constructed based on laboratory biological shock tubes and improvised explosive devices. Although the experimental parameters are controllable, the cost is low, and the repeatability is good, they cannot simulate the complexity and complexity of shock waves under actual explosion conditions. Destructive, there is still a big gap between the damage data obtained in the actual combat environment
[0004] Resveratrol (Resveratrol, RE) is a non-flavonoid polyphenolic compound with a stilbene structure extracted from natural plants, which has various biological effects such as anti-inflammation, anti-oxidation, and immune regulation, but so far There is no report on the use of resveratrol in the treatment of lung blast injury

Method used

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  • Lung knock injury animal model construction method and application thereof
  • Lung knock injury animal model construction method and application thereof
  • Lung knock injury animal model construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Construction of lung blast injury model

[0035] 1. Animal grouping

[0036] The 50 rats were weighed in order and numbered 1-50, using Excel software to generate corresponding random numbers, and the random numbers were arranged in ascending order of size, and the rats were divided into 10 groups in order, with 5 rats in each group, and one group It is the control group (Ctrl group in the figure), and the rest is the test group. The test groups are arranged at different distances from the explosion center.

[0037] 2. Model making

[0038] The model is built with the help of a warhead static explosion power test. The specific operation method is: select explosives with an explosive force of 300kg TNT equivalent (here only 300kg TNT equivalent is used as an example, but it does not mean that only under this explosive force can the lung concussion effect be achieved) for deployment, and then randomly divide the experimental group 45 rats were placed at 15m, 20m, 25m, ...

Embodiment 2

[0069] Evaluation of the effect of resveratrol in alleviating lung blast injury

[0070] 1. Grouping

[0071] Select 40 SD male rats with SPF grade, body weight (200±20) g, and randomly divide them into 4 groups, namely the control group (No treatment, NT), the resveratrol group (Resveratrol, RE), and the model group. group (Blast), model+resveratrol group (Blast+RE), 10 rats in each group.

[0072] 2. Modeling

[0073] Referring to the modeling method in Example 1, the rats in the test group were selected to be placed at a distance of 35 m from the center of explosion. In order to meet the requirements of experimental animal welfare and avoid the influence of stress on the test results, the rats were anesthetized by inhalation of isoflurane before the test. Rats were placed in barbed wire cages and fixed on an arc with a radius of 35 m from the center of explosion. The rats in the control group were far away from the area 3km away from the explosive environment.

[0074]...

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Abstract

The invention relates to the technical field of medicine, in particular to a lung knock injury animal model construction method which can truly reflect physical parameters related to explosion and a complex action process generated by interaction of the physical parameters and can achieve quantitative evaluation on the range, grade and severity of lung injury. A lung knock injury animal model constructed by the method verifies that the resveratrol has a relatively good curative effect in treating lung knock injury, has the advantages of wide source, safety, difficulty in generating drug resistance and the like, and can reduce lung tissue bleeding, alveolar septum rupture and inflammatory cell infiltration of a lung knock injury rat and relieve the lung tissue edema.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for constructing an animal model of lung blast injury and its application. Background technique [0002] Blast lung injury is the most common form of physical injury in military wars, terrorist attacks, and industrial explosion accidents. The lung is one of the most sensitive target organs to shock wave overpressure. The main manifestations of damage are pulmonary hemorrhage, edema, rupture, bullae, collapse, and emphysema. The deterioration of the condition can lead to acute lung injury (acute lung injury, ALI), acute respiratory distress syndrome (acute respiratory distress syndrome, ARDS), multiple organ dysfunction syndrome (multiple organ dysfunction syndrome, MODS), and eventually even death. At present, there is no effective drug for the treatment of BLI, so it is urgent to find a safe and efficient drug for the treatment of BLI. [0003] The damage process of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K31/05A61K49/00A61P11/00
CPCA01K67/02A61K49/0008A61K31/05A61P11/00
Inventor 王鸿高俊宏骞爱荣刘进仁卢青范小琳李亮张文娟刘志永
Owner 兵器工业卫生研究所
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