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Large pore size bilirubin adsorbent, preparation method, and application thereof

A technology of bilirubin and adsorbent, which is applied in the field of medical bioengineering, can solve the problems of small surface and internal pore size, difficulty in controlling the volume of the adsorbent, and poor adsorption effect, so as to increase the surface and internal pore size, increase the amine Base content, the effect of increased adsorption

Inactive Publication Date: 2013-04-03
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most resin adsorbents are currently prepared by suspension polymerization of ionic liquid-water dual-phase / oil dual-phase / reversed-phase systems. The volume of the adsorbent is difficult to control, and the surface and internal pore diameters are small, generally 5-10 μm. Poor adsorption effect

Method used

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  • Large pore size bilirubin adsorbent, preparation method, and application thereof
  • Large pore size bilirubin adsorbent, preparation method, and application thereof
  • Large pore size bilirubin adsorbent, preparation method, and application thereof

Examples

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preparation example Construction

[0037] The equipment used in the present invention to prepare the large-aperture bilirubin adsorbent includes a jet pump, a syringe, a low-temperature freezer, a high-voltage electrostatic instrument, and the jet pump provides power to the syringe to control the aminated cross-linked chitosan solution in the syringe Inject vertically into the low-temperature memory from top to bottom at a certain rate in a high-voltage electrostatic field;

[0038] High-voltage electrostatic instrument used in the present invention: University of Shanghai for Science and Technology;

[0039] Jet pump used: Angel 5805, Shanghai Anjie Electronics;

[0040] The vacuum freeze dryer used: VIRTIS, BT3.3EL;

[0041] Scanning electron microscope used: PHILIPS, XL30 FEG;

[0042] Chitosan: Sigma, USA;

[0043] Ethylenediamine: AR, Shanghai Sinopharm Chemical Reagent Co., Ltd.;

[0044] Formaldehyde: AR, Shanghai Pacific Chemical Company;

[0045] Glutaraldehyde: BR, Shanghai Sinopharm Chemical Rea...

Embodiment 1

[0047] A kind of macroporous bilirubin adsorbent, it is prepared by following method:

[0048] (1), amination of chitosan

[0049] In 10ml concentration is 5% chitosan solution, add 3ml concentration and be 0.08mmol / L

[0050] ethylenediamine aqueous solution, after reacting at 45°C for 4h, add a 5% formaldehyde aqueous solution

[0051] 1ml;

[0052] The chitosan used has a molecular weight of 400,000 and a deacetylation degree of 84-89%;

[0053] (2), cross-linking of chitosan

[0054] Add 1 ml of 0.1% cross-linking agent glutaraldehyde aqueous solution to the aminated chitosan monomer in step (1), control the rotation speed to 120 r / min, and the temperature to 65 ° C for 30 min;

[0055] (3) Low-temperature rapid prototyping under high-voltage static electricity

[0056] Add 15ml of the cross-linked chitosan solution in step (2) into the jet pump, through the jet pump, control the jet speed to 70mm / h, and drop it vertically into the low-temperature freezer at -196°C pl...

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Abstract

The present invention discloses a large pore size bilirubin adsorbent, a preparation method, and an application thereof. According to the large pore size bilirubin adsorbent, a chitosan monomer reacts with an aminating agent ethylenediamine, and free amino groups are protected; then a cross-linking agent glutaral is added, and the resulting mixture is sprayed into a cryopreservation device under a high voltage and static electricity environment to carry out rapid forming to obtain the large pore size bilirubin adsorbent; the prepared large pore size bilirubin adsorbent has uniform particle size, wherein the diameter is about 500 mum; the surface and the inner of the adsorbent particles are rich in pores, wherein the diameter of the pores is about 40 mum and the pores are mutually communicated. With the present invention, the large pore size bilirubin adsorbent of the present invention has good biocompatibility; after the large pore size bilirubin adsorbent is subjected to freeze drying and washing, no chemical residue is remained, wherein the washing treatment is performed three times; the bilirubin adsorption effect of the large pore size bilirubin adsorbent is increased; the adsorbent particle diameter can be regulated during the preparation process; with adopting the adsorbent to carry out in vitro plasma lavage or co-culture, the bilirubin can be effectively absorbed by the amino group, the hydrophobic interaction and the electrostatic interaction; the rich pores on the surface and the inner of the adsorbent increase the filtration area and improve the adsorption efficiency.

Description

technical field [0001] The invention belongs to the field of medical bioengineering, and relates to a large-pore bilirubin adsorbent and a preparation method thereof. Specifically, the aminated chitosan after protecting free amine groups is cross-linked and sprayed into a low-temperature storage device under high-voltage static electricity rapid prototyping. The obtained high amino chitosan microspheres have controllable volume, uniform size, rich surface and internal pore diameters, and good adsorption performance to bilirubin. Background technique [0002] Bilirubin is the metabolite of heme in hemoglobin and other heme proteins in macrophages or other reticuloendothelial cells and hepatocytes. 80%-85% of bilirubin comes from the disintegration of aging red blood cells; about 15% is formed by the destruction of immature red blood cells in the bone marrow during hematopoiesis (ineffective erythropoiesis in the bone marrow); a small amount comes from blood-containing red bl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/24B01J20/28B01J20/30
Inventor 董亚东崔龙彭承宏韩宝三黄芳
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE