Induction method of grateloupia filicina filament

The technology of centipede algae and filamentous body is applied in the field of seedling cultivation of centipede algae, which can solve the problems of inability to become seedlings, fast volatilization of water, inability to use, etc., and solves the bottleneck of seedlings, the induction speed is fast, and the volatilization of water is slow. Effect

Inactive Publication Date: 2012-06-27
NINGBO UNIV
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Problems solved by technology

However, the source of centipede algae is very limited. At present, wild resources are mainly collected. The natural distribution space is small, the quantity is unstable, and the quality is difficult to guarantee. The bottleneck of artificial breeding is the problem of seedlings. Studies on the induction of centipede filaments rapidly cultured with filaments, such as Zhang Zeyu (Journal of Dalian Fisheries University, 2007, Vol. 22, No. 3, 164-169) reported that in a petri dish containing nutrient solution, the Cultivation test of cut pieces of centipede algae (50-250 microns in length) at 10, 15, and 20°C; the test shows that the filaments of cut pieces of centipede algae form the fastest and the lar

Method used

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Examples

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Effect test

Embodiment 1

[0012] A method for inducing centipede filaments, comprising the following steps:

[0013] a. Preparation of induction solution: boil clean seawater and cool to obtain a cleaning solution, add germanium oxide, sodium nitrate and sodium phosphate to the cleaning solution, stir evenly to obtain an induction solution, the concentration of germanium oxide in the induction solution is 2 mg / L, and the induction solution The concentration of sodium nitrate in the medium is 35mg / L, and the concentration of sodium phosphate is 8mg / L;

[0014] b. Disinfection and sectioning: Clean the algae fixer, base and middle part of the main branch of Centipede algae with cleaning solution, soak in hydrogen peroxide with a mass percentage concentration of 7% for 3 minutes, take it out after soaking, and use cleaning solution Rinse the algae fixer, base and middle of the main branch of Centipede algae; then cut the rinsed Algae fixer, base and middle of the main branch of Centipede algae into 0.1-5m...

Embodiment 2

[0018] It is basically the same as Example 1, except that the concentration of germanium oxide in the induction solution is 1 mg / L, the concentration of sodium nitrate in the induction solution is 50 mg / L, and the concentration of sodium phosphate is 5 mg / L. The mass percent concentration of hydrogen peroxide is 5%, and the immersion time of the algal body fixer, base and main branch middle is 5 minutes.

Embodiment 3

[0020] It is basically the same as Example 1, except that the concentration of germanium oxide in the induction solution is 3 mg / L, the concentration of sodium nitrate in the induction solution is 20 mg / L, and the concentration of sodium phosphate is 10 mg / L. The mass percent concentration of hydrogen peroxide is 10%, and the soaking time of the algae fixer, the base and the middle part of the main branch is 2 minutes.

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Abstract

The invention discloses an induction method of a grateloupia filicina filament, which comprises the processes of induction liquid preparation, disinfection and section cutting, induction and culture. Induction liquid is obtained by adding an appropriate amount of nutrient salt and germanium oxide into clean sea water after the clean sea water is boiled and cooled, frond cut sections of grateloupia filicina are induced into an inducing filament in holes of 24 pore plates at the temperature of 22 DEG C and at the illumination intensity of 200-400Lx, and the inducing filament is cultivated into grateloupia filicina seedlings in a triangular flask containing the induction liquid. Due to the fact that induction is performed in the holes of the 24 pore plates where the induction liquid is added, specific surface is small during induction, moisture is volatilized slowly, pollution is little, induction of the filament is facilitated, the induction speed is quick, and the inducing filament of 0.05-0.18mm can be obtained by induction for 4-7 days. Besides, a frond holdfast and frond cut sections of a base portion can be substantially inducted to form the filament, part of the frond cut sections in the middle of a main branch can be induced to form the filament, the induction amount of the filament is large, and the seedling bottleneck problem during artificial culture of grateloupia filicina is solved.

Description

technical field [0001] The invention relates to seedling cultivation technology of centipede algae, in particular to a method for inducing centipede algae filaments. Background technique [0002] Centipede algae (Grateloupia), rich in carrageenan, is used as a thickener and stabilizer in the food industry; as a sizing material in the textile industry; because carrageenan oligosaccharides also have development potential in the field of immunity, it is also an economical food with therapeutic value Seaweed; therefore, the raw material demand for centipede algae is increasing. However, the source of centipede algae is very limited. At present, wild resources are mainly collected. The natural distribution space is small, the quantity is unstable, and the quality is difficult to guarantee. The bottleneck of artificial breeding is the problem of seedlings. Studies on the induction of centipede filaments rapidly cultured with filaments, such as Zhang Zeyu (Journal of Dalian Fisher...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 骆其君谢贞优严小军周成旭朱艺峰徐善良
Owner NINGBO UNIV
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