Induction method of grateloupia filicina filament

Abstract

The invention discloses an induction method of a grateloupia filicina filament, which comprises the processes of induction liquid preparation, disinfection and section cutting, induction and culture. Induction liquid is obtained by adding an appropriate amount of nutrient salt and germanium oxide into clean sea water after the clean sea water is boiled and cooled, frond cut sections of grateloupia filicina are induced into an inducing filament in holes of 24 pore plates at the temperature of 22 DEG C and at the illumination intensity of 200-400Lx, and the inducing filament is cultivated into grateloupia filicina seedlings in a triangular flask containing the induction liquid. Due to the fact that induction is performed in the holes of the 24 pore plates where the induction liquid is added, specific surface is small during induction, moisture is volatilized slowly, pollution is little, induction of the filament is facilitated, the induction speed is quick, and the inducing filament of 0.05-0.18mm can be obtained by induction for 4-7 days. Besides, a frond holdfast and frond cut sections of a base portion can be substantially inducted to form the filament, part of the frond cut sections in the middle of a main branch can be induced to form the filament, the induction amount of the filament is large, and the seedling bottleneck problem during artificial culture of grateloupia filicina is solved.

Description

technical field [0001] The invention relates to seedling cultivation technology of centipede algae, in particular to a method for inducing centipede algae filaments. Background technique [0002] Centipede algae (Grateloupia), rich in carrageenan, is used as a thickener and stabilizer in the food industry; as a sizing material in the textile industry; because carrageenan oligosaccharides also have development potential in the field of immunity, it is also an economical food with therapeutic value Seaweed; therefore, the raw material demand for centipede algae is increasing. However, the source of centipede algae is very limited. At present, wild resources are mainly collected. The natural distribution space is small, the quantity is unstable, and the quality is difficult to guarantee. The bottleneck of artificial breeding is the problem of seedlings. Studies on the induction of centipede filaments rapidly cultured with filaments, such as Zhang Zeyu (Journal of Dalian Fisher...

AI Technical Summary

Problems solved by technology

However, the source of centipede algae is very limited. At present, wild resources are mainly collected. The natural distribution space is small, the quantity is unstable, and the quality is difficult to guarantee. The bottleneck of artificial breeding is the problem of seedlings. Studies on the induction of centipede filaments rapidly cultured with filaments, such as Zhang Zeyu (Journal of Dalian Fisheries University, 2007, Vol. 22, No. 3, 164-169) reported that in a petri dish containing nutrient solution, the Cultivation test of cut pieces of centipede algae (50-250 microns in length) at 10, 15, and 20°C; the test shows that the filaments of cut pieces of centipede algae form the fastest and the largest number at 20°C, and the filaments form at 15°C The fastest growth; after about 2 months of indoor cultivation, the seedlings of centipede algae grow

However, the specific surface area of ​​the petri dish is large, so the water volatilizes quickly and is easy to contaminate, which is not conducive to the induction of filaments; The cut section can grow young shoots, but cannot produce rhizoids), cannot become seedlings, and only a small part can form filaments (the algae fixator can produce rhizoids and become filaments). This induction method is basically the same as natural reproduction , so the current induction method of centipede filaments can not be used in the actual seedling raising process