Preparation for nanometer magnetic immunomicrosphere for enriching circulating cancer cells

Abstract

The invention belongs to the technical field of biomedicine and discloses a nanometer magnetic immunomicrosphere for enriching circulating cancer cells and a use method thereof. A nanometer carbon coated pure ferromagnetic microsphere (Fe@C) is taken as an inner core and is connected with a cancer cell antibody, so as to prepare the nanometer magnetic immunomicrosphere for enriching the circulating cancer cells. By utilizing the magnetic immunomicrosphere to enrich the circulating cancer cells, the sensitivity of the detection for the circulating cancer cells is efficiently increased. The immunomicrosphere has the characteristics of strong magnetic reactivity, easiness in operation, and the like, and is applied to the quick enriching of the circulating cancer cells.

Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to the preparation and application method of nanometer magnetic immune microspheres enriched in circulating tumor cells. technical background

[0002] According to the pathway of tumor cell micrometastasis, tumor cells circulating in the blood or lymphatic vessels are called circulating tumor cells (circulating tumor cells, CTCs), and cell clumps formed by the aggregation of several circulating tumor cells are called circulating tumor microemboli ( Circulating tumor microemboli, CTM). CTM is the "collective migration" behavior of tumor cells, which has higher metastatic potential because of its ability to resist apoptosis and maintain cell proliferation. Due to the very small amount of circulating tumor cells in blood or lymph fluid and the lack of effective specific markers, the research on CTC has been slow. The existing separation and detection techniques have their own a...