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Resistant starch content measuring method for rice

A determination method and technology of resistant starch, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve problems affecting the accuracy of determination results, etc.

Inactive Publication Date: 2012-07-04
SHANGHAI ACAD OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the method uses pancreatic α-amylase during the 16h incubation period on a shaking table, and the starch hydrolyzate undergoes different degrees of fermentation, while the resistant starch cannot be hydrolyzed by amylase, but it can be fermented by bacteria, which affects the accuracy of the measurement results.

Method used

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  • Resistant starch content measuring method for rice
  • Resistant starch content measuring method for rice

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Experimental program
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Effect test

Embodiment 1

[0011] A. The effect of antibiotics on the hydrolysis of slow-digesting starch. It can be seen from Table 1 that in the hydrolysis system of α-amylase at 37°C, the glucose concentration in the hydrolyzate of the treatment and the control group showed an increasing trend with the prolongation of the hydrolysis time. The response time of the treatment group was higher than 8 hours in 16 hours. Under the same hydrolysis time, the glucose content in the hydrolyzate of the antibiotic group was significantly higher than that of the control group. At the same time, it was found that after 16 hours of hydrolysis reaction, the glucose content of the hydrolyzed solution of the cephalexin group was the highest, followed by the penicillin G group, the third highest was the erythromycin lactobionate group, and the glucose content of the gentamicin sulfate group was the lowest. In addition, different doses of antibiotic concentrations have significant differences in the glucose content of ...

Embodiment 2

[0023] Technical scheme of the present invention is:

[0024] A. There are 6 treatments and controls in the experiment. The test set penicillin group (diluted to 0.48mg / mL), gentamicin sulfate group (diluted to 1600UI / mL), cephalexin group (diluted to 1.0mg / mL), erythromycin lactobionate group (diluted to 0.1mg / mL), ceftriaxone sodium group (diluted to 1.0mg / mL), chloramphenicol (diluted to 0.5mg / mL) group with 6 treatments and distilled water as a control. Accurately weigh 0.1000g of rice flour (Jiangtangdao No. 1) passed through a 100-mesh sieve, add 40mg of α-amylase (Sigma A-3176), and then add 4mL of sodium maleate buffer solution (0.1mol / L, pH6.0) , add 10 μL 3300UI / mL amyloglucosidase (AGM) to treat it into 4 mL of starch hydrolysis mixture, add 35 μL of 6 kinds of antibiotics to the reaction system, and add 35 μL of distilled water to the starch hydrolysis mixture as a control. Glucose content was detected at 37°C at the 8th hour and 16th hour of enzymatic digestion...

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Abstract

The invention provides an effective method for accurately and objectively measuring resistant starch content in rice by adding different types of antibiotics into starch hydrolyzate to discuss the effect of the antibiotics on starch hydrolysis and influence on a measuring result of the resistant starch content. By comparing the influences of the antibiotics on the starch hydrolysis effect and the resistant starch content of a detected sample, a result shows that the fermentation effect of miscellaneous bacteria in the starch hydrolysis process can be effectively suppressed by the antibiotics without affecting the activity of amylase; the most effective antibiotic is mixed antibiotic (four antibiotics, namely 0.40-0.52 mg / mL of penicillin, 15,000-17,000 UI / mL of gentamicin sulphate, 0.8-1.2 mg / mL of cefalexin and 0.85-1.30 mg / mL of erythromycin lactobionate, are mixed according to a proportion of 1.2:1:1.2:1 ); and the best dose is 35 microlitre.

Description

technical field [0001] The invention relates to the technical field of measuring methods, in particular to a method for measuring resistant starch content in rice. Background technique [0002] The concept of resistant starch (Resistant starch, RS) was first proposed by Engrist (Englist), which refers to the general term for starch and its degradation products that cannot be digested and absorbed in the small intestine of healthy humans. Resistant starch has very important physiological functions, such as reducing postprandial blood sugar levels in diabetic patients, lowering blood lipids and weight control, preventing intestinal diseases, and promoting the absorption of trace elements. [0003] At present, there are mainly enzyme-direct method (Englyst method, Champ method), ultrasonic-enzyme method (Berry method), microwave-enzyme method (Goni method), in vitro method (McCleary method) and so on. Among them, the in vitro assay (McCleary) method has been the preferred meth...

Claims

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Application Information

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IPC IPC(8): C12Q1/40
Inventor 朴钟泽白建江朱辉明
Owner SHANGHAI ACAD OF AGRI SCI
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