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Trichoderma harzianum expansin and coding gene and application thereof

A technology for expanding proteins and genes, applied in the field of molecular biology, can solve the problems of no cellulase activity, difficult detection of activity purification, etc., and achieve obvious synergistic effects and improve degradation efficiency

Inactive Publication Date: 2012-07-18
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Application Information

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Problems solved by technology

[0003] Expansin was originally discovered in plants, and studies have shown that it can break the hydrogen bonds between cellulose microfibrils or between cellulose and other cell wall polysaccharides, but it does not have cellulase activity, making it difficult to detect activity and purify for study its mechanism of action

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  • Trichoderma harzianum expansin and coding gene and application thereof
  • Trichoderma harzianum expansin and coding gene and application thereof
  • Trichoderma harzianum expansin and coding gene and application thereof

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Embodiment

[0023] Material preparation:

[0024] The Trichoderma harzianum described in the examples, the strain number is CGMCC No.3.5364, was purchased from China General Microorganism Culture Collection and Management Center.

[0025] 1. Mycelia Preparation

[0026] Trichoderma harzianum spore suspension (1.00±0.05)×10 7 cells / ml inoculated on coated fiber plates, cultured for 36 hours, collected mycelia in several 1.5ml centrifuge tubes, and stored in liquid nitrogen quick-frozen.

[0027] The composition of the fiber plate is as follows: 55wt% powder (the mass ratio of bran in the powder: grass powder is 1:1), 1.5wt% agar, and the balance of water.

[0028] 2. Extraction of total RNA and separation and purification of mRNA

[0029] Weigh 0.2 g of mycelium in a pre-cooled mortar and grind it to powder with liquid nitrogen. The RNA extraction method and the mRNA separation and purification method were operated according to the corresponding instructions about RNeasy Mini Kit and O...

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Abstract

The invention relates to a trichoderma harzianum expansin and a coding gene and application thereof. The amino acid sequence of the trichoderma harzianum expansin is shown as SEQ ID No.1, and the nucleotide sequence of the gene for coding the expansin is shown as SEQ ID No.2. The prepared expansin has an obvious synergic effect when an insoluble cellulose substrate is degraded by cellulase, the maximum synergic index is 2.63, and the degradation efficiency of the cellulase can be effectively improved.

Description

technical field [0001] The invention relates to a Trichoderma harzianum expansin and its encoding gene and application, belonging to the technical field of molecular biology. Background technique [0002] In the 21st century, human beings are facing the greatest challenge of resource scarcity. Using modern biotechnology to develop biological resources is an important way to solve resource problems. Cellulose, which exists in the cell wall, is the most widely distributed and most abundant polysaccharide in nature. It is the main component of plant cell walls and the largest renewable carbon source on the earth. Its degradation is the central link of the carbon cycle in nature. . It is estimated that the cellulose produced by the photosynthesis of green plants on the earth can reach as much as 1011 tons every year, while the current global food production is only 2.2×10 9 Ton, how to convert cellulose into food or effective energy that can be used by humans is a major issue ...

Claims

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Application Information

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IPC IPC(8): C07K14/37C12N15/31C12N15/63C12N1/15C12N1/21C12P19/14C12R1/885
Inventor 姚强宫志远韩建东王琦李瑾孙涛万鲁长任鹏飞任海霞
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI