Preparation method and application of tumor-targeted photosensitive immunoconjugate

An immunoconjugate and tumor-targeting technology, which is applied to the preparation method of peptides, anti-tumor drugs, chemical instruments and methods, etc., can solve the problems of poor tumor selectivity, phototoxicity, and long-term light-shielding time for patients, and achieve High anti-tumor activity, good stability and low cost
CN102585003AActive Publication Date: 2012-07-18FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
Publication Date
2012-07-18

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Abstract

The invention provides a preparation method of high-activity zinc phthalocyanine-monoclonal antibody tumor-targeted photosensitive immunoconjugate and a technical method for anti-tumor application of the immunoconjugate. Monocarboxyl zinc phthalocyanine with a single structure is reacted with lysine hydrazine pentamer to form a water-soluble monocarboxyl zinc phthalocyanine hydrazine derivative with high reaction activity; meanwhile, the monoclonal antibody with anti-tumor activity is selected; under a neutral condition, glycosyl non-related to activity of the antibody is mildly oxidized into an aldehyde group with sodium periodate; and at 4 DEG C, excess monocarboxyl zinc phthalocyanine hydrazine derivative is extremely easy for reacting with the aldehyde group and the reaction product is further reduced into stable photosensitive immunoconjugate to obtain the high-purity photosensitive immunoconjugate. The preparation method has the characteristics of low temperature, neutral conditions and no use of any organic solvent. In-vitro anti-tumor experiments prove that the conjugate has extremely high selectivity and anti-tumor activity as well as application value.
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Description

technical field

[0001] The invention relates to the fields of medicine, biotechnology, chemistry and immunobiology. Background technique

[0002] In recent years, more and more domestic and foreign literatures have reported a new tumor treatment method—photodynamic therapy (PDT). Tumor photodynamic therapy is the latest development in the field of minimally invasive or non-invasive treatment of modern tumors. It was approved by the US FDA for clinical application in 1996, and has been used in various tumor treatments in the United States, Japan, the United Kingdom, France, Germany, Canada and other countries. success. In May 2003, China SFDA approved the clinical application of this treatment system.

[0003] Photodynamic therapy is a new type of treatment for malignant tumors under development, and it has been used clinically for the treatment of various tumors. In order to increase the photodynamic efficacy, we use a monoclonal antibody that can specifically bind to tum...

Claims

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