Fluorescent quantum dot/nano-metal particle conjugate and preparation and application thereof

A technology of nano metal particles and fluorescent quantum dots, applied in the field of nanomaterials, can solve problems such as expensive and lengthy sample preparation

Inactive Publication Date: 2012-07-18
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional metal ion detection methods, such as chromatography, atomic absorption spectrometry, etc., require expensive instruments and lengthy sample preparation, analysis testing and data processing steps, making it difficult for samples (such as water, food, beverages, etc.) real-time on-site detection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Preparation of single-stranded DNA probe-modified gold nanoparticles

[0063] The synthesis of gold nanoparticles can be found in the methods of the prior art (J.J.Storhoff, R.Elghanian, R.C.Mucic, C.A.Mirkin, R.L.Letsinger.J.Am.Chem.Soc., 1998, 120, 1959-1964). Add 98 ml of water to a two-necked bottle. Add 2 ml of 50 mM HAuCl 4 The solution makes HAuCl in the bottle 4 The concentration was 1 mM, heated to reflux with stirring, then 10 ml of 38.8 mM sodium citrate solution was rapidly added, and the color changed from light yellow to dark red within 1 min. Reflux was continued for 20 minutes, heating off and cooling to room temperature (23-25 ​​degrees Celsius) with stirring. The obtained nanoparticles had a particle size of 13 nm and a concentration of 10 nM. Transmission electron microscopy (TEM) showed that the obtained nanoparticles were spherical.

[0064] Since the sodium citrate-modified gold nanoparticles were unstable at high salt concentration...

Embodiment 2

[0065] Example 2: Preparation of single-stranded DNA probe-modified fluorescent quantum dots

[0066] Fluorescent quantum dots were prepared by one-step reaction (see: Q.B.Wang, D.K.Seo.JMater Sci., 2009, 44, 816; Q.B.Wang, Y.Liu, Y.Ke, H.Yan.Angew.Chem., Int. Ed., 2008, 47, 316; Q.B.Wang, N.Iancu, D.K.Seo.Chem.Mater., 2005,17:4762; and Q.B.Wang, Y.Xu, X.Zhao, Y.Chang, Y.Liu, L. Jiang, J. Sharma, D. K. Seo, H. Yan. J. Am. Chem. Soc., 2007, 129, 6380).

[0067] Specifically, MPA-modified CdSe / ZnS core / shell quantum dots were prepared as follows. Put 0.1 mmol P 2 S 5 , 0.5 g of 3-mercaptopropionic acid (MPA) and 0.3 ml of butylamine were mixed in 10 ml of N-methyl-2-pyrrolidone (NMP) and heated to dissolve. After cooling to room temperature, 0.01 g of oleylamine-modified CdSe core particles (provided by Beijing Zhongke Wuyuan Biotechnology Co., Ltd., with a fluorescence peak of 525 nm) were added, and then mixed with 0.05 g of ZnCl 2 solution mix. The mixed solution was he...

Embodiment 3

[0070] Example 3: Preparation of Fluorescent Quantum Dot / Nano Metal Particle Conjugates

[0071]In the QD-DNA solution from Example 2 with a QD concentration of 200 nM, an Au-DNA solution with a particle size of 13 nm from Example 1 with a concentration of Au of 8 nM was added, where QD:Au=Au=QD in the QD-Au structure was maintained 5:1, that is, 2 microliters of QD-DNA solution and 10 microliters of Au-DNA solution, and then add DNase (40 μM, 0.1 microliters) and the corresponding substrate DNA (40 μM) to the system. , 0.1 μl) and sodium chloride solution to a concentration of 0.15 mM sodium chloride, and annealed the hybridization overnight. The annealing curve for DNA hybridization was held at 80°C for 5 minutes and then slowly cooled to room temperature.

[0072] When the target is lead, the DNase is 5'-CAT CTC TTC TCC GAG CCGGTC GAA ATA GTG AGT-3' and the substrate DNA is 3'-GTG CTC AAC TGTGTA GAG AAG GrA TAT CAC TCA AGT GTC TAC TCA-5'.

[0073] When the target is copp...

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Abstract

The invention relates to a fluorescent quantum dot/nano-metal particle conjugate and a preparation method thereof, a composition and an elisa kit containing the conjugate and an application of the conjugate in detecting target objects especially in detecting metal ions.

Description

technical field [0001] The invention relates to nanomaterials, in particular to a fluorescent quantum dot / nano metal particle conjugate, its preparation method and its application in detecting metal ions. Background technique [0002] In the environment (especially in fresh water), the pollution of metal ions such as mercury, lead, copper, manganese, cadmium, chromium, etc., has great harm to human health and sustainable economic development. Therefore, the detection of metal ions, especially the real-time and high-sensitivity metal ion detection in the field, is increasingly important. However, traditional metal ion detection methods, such as chromatography, atomic absorption spectrometry, etc., require expensive instruments and lengthy sample preparation, analytical testing and data processing steps, which are difficult to perform on samples (such as water, food, beverages, etc.). Real-time on-site inspection. [0003] Quantum dots (Quantum Dot, QD), also known as nanocr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/52B82Y20/00
Inventor 王强斌徐冰梶浦尚志李勇明
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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