Method for extracting acid mucopolysaccharide and sodium hyaluronate from freshwater mussel gravy

Abstract

The invention discloses a method for extracting acid mucopolysaccharide and sodium hyaluronate from freshwater mussel gravy. Through the processes of enzymolysis, complex precipitation, centrifugation, ultrafitration classification and vacuum drying in the adopted extraction method, the damage caused in the process of degrading sodium hyaluronate is reduced, the macromolecular characteristic of sodium hyaluronate is remained, sodium hyaluronate is closer to HA (Hyaluronic Acid) required by a human body, and two-stage products including the acid mucopolysaccharide with the content of more than 90% and the finished sodium hyaluronate with the glucuronic acid content of more than 43% can be obtained in sequential extraction. The HA extracted by using the method disclosed by the invention is wide in raw material source, low in price, safe and reliable and capable of replacing the HA extracted by taking cockscombs and human umbilical cords as raw materials, and the production yield of natural HA can be greatly increased. The method disclosed by the invention is simple and convenient to operate, high in separation efficiency and free of phase change and can be widely applied to the fields of medicines, aesthetic health care and health foods.

Description

Technical field: [0001] The invention relates to the field of biotechnology for extracting macromolecular sodium hyaluronate (HA) and acidic mucopolysaccharide from animal tissues, and more specifically relates to a process for extracting acidic mucopolysaccharide and sodium hyaluronate in freshwater clam gravy. Background technique: [0002] Sodium hyaluronate (HA) is also known as hyaluronic acid and hyaluronic acid. It is a kind of acidic mucopolysaccharide. With its unique molecular structure, sodium hyaluronate is widely used in cardiovascular surgery, arthritis, surgical anti-adhesive agent, drug delivery, cancer treatment, ophthalmology, plastic surgery and cosmetic maintenance, etc. . At present, the production of hyaluronic acid products is divided into two methods: extraction method and microbial fermentation method. The extraction method produces sodium hyaluronate with rooster comb and human umbilical cord as the raw materials for extraction. The two raw materia...

AI Technical Summary

Problems solved by technology

At present, the production of hyaluronic acid products is divided into two types: extraction method and microbial fermentation method. The extraction method produces sodium hyaluronate using rooster comb and human umbilical cord as the raw materials for extraction. The two raw materials are limited by the source, so the output is scarce and the price is expensive. Can not meet market demand

The traditional chloroform extraction process is complicated to operate, and chloroform is not friendly to the environment. If an agricultural and sideline product with abundant resources, safety and reliability, and low raw material cost is used as the extraction raw material and extraction process of sodium hyaluronate, the hyaluronic acid will be greatly reduced. Reduce the production cost of sodium hyaluronate, increase the output of sodium hyaluronate, and increase the source of economic income for farmers in water towns