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Method and device for extracting polyphenol substance from Korean pine shells

A technology of Korean pine shells and Korean pine, which is applied in the field of chemical extraction of agricultural natural products, can solve the problems of low extraction rate of polyphenols and increased yield of polyphenols, etc.

Inactive Publication Date: 2012-08-15
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem of low extraction rate of polyphenols in Korean pine shells, through the research and determination of multiple process parameters in the process of enzymatic hydrolysis, ultrasonic extraction, and purification and separation, to study a method of extracting polyphenols from Korean pine shells. Method and equipment for polyphenolic substances to achieve the purpose of increasing the yield of polyphenols

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  • Method and device for extracting polyphenol substance from Korean pine shells
  • Method and device for extracting polyphenol substance from Korean pine shells
  • Method and device for extracting polyphenol substance from Korean pine shells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Enzymatic hydrolysis process optimization

[0038] 1 Materials and methods

[0039] 1.1 Raw materials and reagents

[0040] Raw materials: Pine husks were collected in Yichun, Heilongjiang Province, sieved with 40 mesh after crushing, dried at low temperature to constant quality and used for later use.

[0041] Reagents: Cellulase (Sanland-chem International Inc, 30u / mg), gallic acid, Folin reagent, sodium carbonate, concentrated hydrochloric acid, sodium hydroxide, and absolute ethanol were all domestic analytical grades.

[0042] 1.2 Instruments

[0043] High-speed universal pulverizer: Tianjin Tester Instrument Company; constant temperature water bath: Beijing Medical Electronic Instrument Factory; acidity meter: Shanghai Dazhong Analytical Instrument Factory; T6 New Century UV-Vis Spectrophotometer: Beijing Puyan General Company; R-205B Rotary Evaporator: Shanghai Shensheng Instrument Company; Electronic Balance: Xi'an Xingchengzhi Instrument;

[0044] 1...

Embodiment 2

[0086] Embodiment 2 Ultrasonic extraction process optimization

[0087] 2.1 Experimental method

[0088] 2.1.1 Single factor test of polyphenol extraction from Korean pine seed husk

[0089] 2.1.1.1 Investigation of ultrasonic temperature factors

[0090] Accurately weigh 7 parts of 2.00g Korean pine seed husk powder into a 100mL beaker, add 61% ethanol as an extraction solvent, place it in an ultrasonic crusher, and press 30°C, 40°C, 50°C, 60°C, 70°C, Ultrasonic extraction was carried out at 80 °C for 2.5 h. The samples extracted under each temperature condition were collected and centrifuged immediately at 4000 rpm for 10 min. Collect the supernatant in a conical flask for testing. After the samples extracted under each temperature condition were collected completely, the polyphenol content was determined according to the Folin phenol method and the polyphenol yield was calculated.

[0091] 2.1.1.2 Investigation of ultrasonic time factor

[0092] Accurately weigh 6 par...

Embodiment 3

[0122] Through the SAS9.2 software, the optimal extraction process was obtained through the fitting analysis of the response surface optimization process results: ultrasonic extraction time 2.55h, ultrasonic temperature 62 ℃, ultrasonic power 820W. The predicted value of polyphenol extraction rate by response surface model was 8.92mg / g under this condition. For further verification, three parallel experiments were carried out using the determined optimized conditions, and the results showed that the average polyphenol extraction yield was 8.54mg / g, indicating that the experimental results were in good agreement with the model, and reached the highest yield during the experiment, indicating that the response surface The model is feasible. Example 3 Optimization of purification process for Korean pine seed shell polyphenols

[0123] 3.1 Experimental method

[0124] 3.1.1 Macroporous resin pretreatment

[0125] Soak macroporous resin AB-8 in ethanol for 24 hours, fully swell a...

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Abstract

The invention relates to a method and device for extracting a polyphenol substrate from Korean pine shells, belonging to the chemical extraction technology of the forestry natural products. The method comprises the steps of: based on Korean pine shells as the raw materials, carrying out superfine pulverization treatment, then mixing with water to obtain a mixture solution, adding cellulase into the mixture solution for enzymoloysis, carrying out centrifugal separation to obtain a supernatant and residues, taking the supernatant, adding ethanol, then ultrasonically extracting, and separating and purifying the extraction solution a macroporous adsorptive resin, so as to obtain the polyphenol substrate. The device comprises an enzymolysis tank, a centrifuge, an ultrasonic extraction tank, a distillation tower, a separation and purification column, an ethanol recovery tank and a condenser. The method and device have the characteristics of novel and reasonable design, simple process, mild condition, and high extraction rate of the polyphenol substance.

Description

technical field [0001] The invention belongs to the chemical extraction technology of agricultural natural products, and mainly relates to a method and equipment for extracting polyphenols from Korean pine shells. Background technique [0002] Korean pine (Pinus koraiensis), also known as fruit pine, sea pine, evergreen tree, up to 40m. The leaves are in a bunch of five needles, 6-12cm long. Distributed from Changbai Mountain to Xiaoxing'an Mountains in Northeast my country, it often forms mixed forests with fish scale pine and red spruce. It is an important agricultural and forestry resource. Its fruit, Korean pine seeds, are famous for their plump particles and rich nutrition. High medicinal value and health care function. Studies have shown that pine plants contain a variety of biologically active components such as terpenes, polyphenols, alkaloids, etc., which have biological activities such as anti-oxidation, anti-tumor and antibacterial. Among them, polyphenols are im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/15C12P1/00A61P39/06
Inventor 王振宇黄雨洋
Owner NORTHEAST FORESTRY UNIVERSITY
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