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Method and device for preparing functional nanoparticle/bacterial cellulose composite membranes

A technology of bacterial cellulose and functional nanometers, applied in fermentation and other directions, can solve the problems of weak adsorption force of functional nanoparticles, and achieve the effects of low cost, good adsorption and short cycle.

Inactive Publication Date: 2012-08-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The specific method is to add functional nanoparticles to the bacterial cellulose fermentation medium, and obtain a stable functional nanoparticle suspension by stirring and adding surfactants. The bacterial cellulose cultivated in such a fermentation medium can The functional nanoparticles are completely covered, and through appropriate dispersion means, the functional nanoparticles can be evenly distributed on the surface and inside of the bacterial cellulose membrane, so as to obtain excellent performance without destroying the three-dimensional structure inside the bacterial cellulose membrane. The functionalized bacterial cellulose composite membrane solves the general problem of preparing functionalized bacterial cellulose membranes by conventional methods, that is, functional particles can only be composited on the surface of bacterial cellulose membranes and cannot easily enter the interior of the membrane and functional particles and bacterial fibers The problem of weak adsorption of plain film to functional nanoparticles

Method used

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  • Method and device for preparing functional nanoparticle/bacterial cellulose composite membranes
  • Method and device for preparing functional nanoparticle/bacterial cellulose composite membranes

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1. Prepare seed medium for activating Acetobacter. The composition of the seed medium is: glucose 7.0w / v%, peptone 0.6w / v%, citric acid monohydrate 0.3w / v%, potassium dihydrogen phosphate 0.2w / v%, sodium hydrogen phosphate dodecahydrate 0.3w / v%, yeast extract 0.5w / v%. Control the pH value of the seed medium to 7.0, and sterilize at 121°C for 30 minutes.

[0040] 2. Take out the acetogenes stored in the freezer. Heat the inoculation loop on the alcohol lamp, extend the inoculation loop into the inclined surface of the test tube, hook one loop of the strain and insert it into the Erlenmeyer flask containing the seed medium as described in step 1. Seal the Erlenmeyer flask into which the strain was inserted with gauze, put it into a shaker for activation at 30° C. for 24 hours, and the shaker rotates at 160 rpm.

[0041] 3. Prepare a fermentation medium containing iron ferric oxide nanoparticles in a culture vessel for cultivating a functional nanoparticle / bacterial ce...

Embodiment 2

[0046] 1. Prepare seed medium for activating Bacillus aceti. The composition of the seed medium is: glucose 5.0w / v%, peptone 0.4w / v%, citric acid monohydrate 0.1w / v%, potassium dihydrogen phosphate 0.1w / v%, sodium hydrogen phosphate dodecahydrate 0.1w / v%, yeast extract 0.4w / v%. Control the pH of the seed medium to 6.0, and sterilize at 121°C for 30 minutes.

[0047] 2. Take out the acetobacteria stored in the freezer. Heat the inoculation loop on the alcohol lamp, extend the inoculation loop into the inclined surface of the test tube, hook one loop of the strain and insert it into the Erlenmeyer flask containing the seed medium as described in step 1. Seal the Erlenmeyer flask into which the strain was inserted with gauze, put it into a shaker for activation at 30° C. for 24 hours, and the shaker rotates at 160 rpm.

[0048] 3. Prepare a fermentation medium containing temperature-induced reversible discoloration microcapsules with gum arabic and gelatin as the wall materia...

Embodiment 3

[0053] 1. According to the cultivation process described in the doctoral dissertation "Preparation and Structural Properties of Bacterial Cellulose / Carbon Nanotube Composite Materials", the seed medium and fermentation medium were prepared for the activation of Acetobacter xylinum and the bacterial fiber of Acetobacter xylinum respectively. prime cultivation.

[0054] 2. In the step of preparing the fermentation medium, add nano-silver particles to the fermentation medium, the particle diameter of which is 60-120nm, and the added mass accounts for 5.0wt% of the total mass of the fermentation medium.

[0055] 3. During cultivation, seal the culture container with an oxygen-permeable silicone membrane and place it upside down on the airtight oxygen chamber, and seal the joint between the culture container and the airtight oxygen chamber with paraffin. After standing for 1 h, oxygen was introduced into the oxygen chamber. Static culture was carried out at 30°C for 14 days.

[0...

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Abstract

The invention relates to a method and device for preparing functional nanoparticle / bacterial cellulose composite membranes by an inverted culturing method. The method comprises the steps of preparing a culture medium, carrying out activation and accessing on strains, and carrying out culturing and post-processing on bacterial cellulose membranes, wherein in the step of preparing the culture medium, functional nanoparticles are added. Inverted culturing refers to inversely place a culturing vessel on an oxygen chamber, the culturing vessel and the oxygen chamber are separated by using an oxygen permeating membrane, and a seam between the culturing vessel and the oxygen chamber is sealed. The device disclosed by the invention comprises a base and a culturing vessel, wherein the base is an open vessel with a step at the upper part thereof, the culturing vessel is inversely placed on the step of the vessel, the base and the culturing vessel are separated by using an oxygen permeating membrane, the oxygen permeating membrane and the lower part of the base form an oxygen chamber, and the lower part of the base is provided with an air outlet hole and an air inlet hole. The method and device disclosed by the invention integrate the culturing and functionalization of bacterial cellulose membranes, and are simple in operation, short in cycle and low in cost; and according to the invention, on the premise of not damaging a three-dimensional network structure in a bacterial cellulose membrane, a functionalized bacterial cellulose composite membrane which is good in functional-nanoparticle adsorption property and excellent in performance is prepared.

Description

technical field [0001] The invention relates to a method and a device for preparing a functional nanoparticle / bacterial cellulose composite film, in particular to a method and a device for preparing a functional nanoparticle / bacterial cellulose composite film by an inverted culture method. Background technique [0002] As a new type of biological material, bacterial cellulose has many unique properties, such as ultrafine network fiber structure, high crystallinity and high chemical purity, strong water holding capacity and air permeability, high Young's modulus, good biocompatibility These properties make bacterial cellulose widely used in various fields. The multifunctionalization of bacterial cellulose can be realized by compounding bacterial cellulose with various functionalized particles. [0003] At present, there are mainly the following methods for the functionalization of bacterial cellulose: in-situ composite method, physical adsorption method, and physical blendin...

Claims

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Application Information

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IPC IPC(8): C08L1/02C08K3/22C08K9/10C08K3/08C12P19/04
Inventor 郑羿王肖杨敬轩汤廉项草郑威力陈仕艳王华平
Owner DONGHUA UNIV
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