Weeding composition for rice direct-seeding field

[0025] Formulation Example 2: 60% pretilachlor·pyrazosulfuron-methyl·clomazone water dispersible granules.

[0026] Pretilachlor 28%, pyrazosulfuron 4%, clomazone 28%, sodium lauryl sulfate 4%,

[0027] Carboxylate 5%, urea 10%, add kaolin to 100%.

[0028] Add the above materials to the cone mixer and mix them evenly, and then pulverize by the jet mill. The crushed materials are mixed by the cone mixer. The fineness of the mixed materials is 98% through the 325 mesh standard sieve and added to the kneader. Kneaded into plastic materials, and finally put the materials into an extrusion granulator to extrude and granulate. After granulation, they are dried and sieved to obtain 60% pretilachlor·pyrazosulfuron-methyl·clomazone water dispersion Granules.

[0029] Formulation Example 3: 38% pretilachlor, pyrazosulfuron-methyl, clomazone wettable powder.

[0030] Pretilachlor 10%, pyrazosulfuron 2%, clomazone 26%, sodium lauryl sulfate 2%,

[0031] Sodium butyl naphthalene sulfonate 3%, calcium lignosulfonate 5%, white carbon black 5%, kaolin to 100%.

[0032] Add the above materials to the cone mixer and mix them evenly, and then pulverize by the jet mill. The crushed materials are then mixed by the cone mixer. The fineness of the mixed materials is 98% and passes through the 325 mesh standard sieve, which is 38%. Pretilachlor, pyrazosulfuron-methyl, clomazone WP.

[0033] Formulation Example 4 :3% Pretilachlor·Pyrimsulfuron·Clomazone Granules.

[0034] Pretilachlor 1.0%, pyrazosulfuron 0.1%, clomazone 1.9%, sodium lauryl sulfate 4%, calcium lignosulfonate 5%, attapulgite 30%, and kaolin to 100%.

[0035] Add the above-mentioned materials to a cone mixer and mix them evenly, and then pulverize them by a jet mill. The crushed materials are mixed by a cone mixer. The fineness of the mixed materials is 98% through a 325-mesh standard sieve to obtain wettability. powder. Put this wettable powder into a disc granulator and add water to granulate, then dry it in a drying cylinder, sieving, and cooling in a cooling cylinder to obtain 3% pretilachlor·pyrazosulfuron·clomazone granules.

[0036] Bioassay Example 1.

[0037] In order to verify the present invention, we conducted a virulence test of pretilachlor, pyrazosulfuron-methyl, and clomazone compound paired with barnyardgrass and duck diffusa.

[0038] 1 Materials and methods.

[0039] 1.1 Test agents: pretilachlor (pretilachlor) 94% original drug; pyrazosulfuron-etyl (pyrazosulfuron-etyl) 97% original drug; clomazone (clomazone) 93% original drug.

[0040] 1.2 Test material: Echinochloa crusgalli (L.) Beauv.]. Ampatia [Monochoria vaginglis (Burm.f.) Preslex Kunth].

[0041] The above weed seeds were collected from the direct-seeded rice field in Laokeng Village, Renshan Town, Huidong County, Huizhou City, Guangdong Province, and kept indoors for later use.

[0042] 1.3 Test method.

[0043] 1.3.1 Experimental treatment design.

[0044] (1) Pretilachlor 60g (a.i.)/hm 2.

[0045] (2) Pretilachlor 80g (a.i.)/hm 2. (3) Pretilachlor 100g (a.i.)/hm 2. (4) Pretilachlor 120g (a.i.)/hm 2. (5) Pretilachlor 140g (a.i.)/hm 2. (6) Pyrimsulfuron 4g (a.i.)/hm 2. (7) Pyrimsulfuron 6g (a.i.)/hm 2. (8) Pyrimsulfuron 8g (a.i.)/hm 2. (9) Pyrimsulfuron 10g (a.i.)/hm 2. (10) Pyrimsulfuron 12g (a.i.)/hm 2. (11) Chlorophyll pine 20g (a.i.)/hm 2. (12) Chlorophyll pine 40g (a.i.)/hm 2. (13) Crimson pine 60g (a.i.)/hm 2. (14) Chlorophyll pine 80g (a.i.)/hm 2. (15) Cloma pine 100g (a.i.)/hm 2. (16) Pretilachlor 60g + pyrazosulfuron 12g + clomazone 100g (a.i.)/hm 2. (17) Pretilachlor 80g+pyrazosulfuron 10g+ clomazone 80g (a.i.)/hm 2. (18) Pretilachlor 100g+pyrazosulfuron-methyl 8g+ clomazone 60g (a.i.)/hm 2. (19) Pretilachlor 120g+pyrazosulfuron 6g+ clomazone 40g (a.i.)/hm 2. (20) Pretilachlor 140g + pyrazosulfuron 4g + clomazone 20g (a.i.)/hm2. (21) CK

[0046] 1.3.2 The activity test of the agent on barnyardgrass.

[0047] According to the design treatment dosage of 1.3.1, prepare the pharmaceutical solution, first calculate the dosage of the agent according to the experimental design, and then weigh the pretilachlor, pyrazosulfuron-methyl, and clomazone with an electronic balance to dissolve it with acetone, and then use 0.1% Tween 80 aqueous solution was prepared and diluted for later use.

[0048] Barnyardgrass seeds are soaked and germinated, sowed on a cross-sectional area of ​​0.25m filled with soil 2 In the iron pot, 100 seeds per pot were sown, and the barnyardgrass 1 leaf and 1 pistil stage was cultivated in the greenhouse for treatment. Use a hand-held compression sprayer (3NY-1.2) to spray the liquid medicine, the spray amount is 50ml per treatment, keep the soil moist after the medicine, and set a treatment control without medicine. Each treatment was repeated 4 times. The fresh weight of the barnyard grass in each treatment was weighed 20 days after the treatment. The growth inhibition percentage of the above-ground part of each treatment and the remaining barnyard grass were calculated as the percentage of the control. The Golby method was used to evaluate the effect of the three mixtures on barnyard grass. Combination type. The actual control effect of mixed herbicides is calculated according to the following formula:

[0049] In the formula, A represents the control effect when the amount of herbicide I is P, B represents the control effect when the amount of herbicide II is Q, C represents the control effect when the amount of herbicide III is R, and E0 represents the mixture (Ⅰ+ Ⅱ+Ⅲ) The theoretical control effect when the dosage is (P+Q+R), and E represents the actual control effect of each treatment.

[0050] evaluation standard. E- E 0 ﹤-10%, each single agent composing the mixture has an antagonistic effect. E- E 0 =-10~10%, each single agent composing the mixture has an additive effect. E- E 0 ﹥10%, each single agent composing the mixture has a synergistic effect.

[0051] 1.3.3 Activity test of medicament on duck diffusa.

[0052] After soaking the duck's diffusa seeds, germinate them and place them in an iron pot with a cross-sectional area of ​​0.25m2 filled with soil. The rest is the same as 1.3.2.

[0053] 2 Result analysis.

[0054] From the data in Table 1 and Table 2, it can be seen that five different combinations of pretilachlor, pyrazosulfuron-methyl, and clomazone have an effect on the E-E of barnyard grass 0 The values ​​are -7.5%, -2.5%, -1.9%, 1.1%, 3.3%; the E-E0 values ​​of duck diffusa are -5.6%, -1.5%, 2.0%, 2.3%, 3.6%. All are between ±10%. It shows that the combined effect of pretilachlor, pyrazosulfuron-methyl, and clomazone on barnyard grass and duck diffusa is an additive effect.

[0055] 3 Conclusion.

[0056] The weeding spectrum of pretilachlor, pyrazosulfuron-methyl, and clomazone can complement each other, and the combined effect of barnyardgrass and duck diffusa on the ternary mixture is additive. Therefore, it is feasible to mix the three to control annual weeds in rice fields.

[0057] Table 1. Determination of the combined effect of pretilachlor, pyrazosulfuron-methyl, and clomazone on barnyardgrass (20 days after treatment).

[0058]

[0059] Table 2. Determination of the combined effect of pretilachlor, pyrazosulfuron-methyl, and clomazone on barnyardgrass (20 days after treatment).

[0060]

[0061] Bioassay Example 2: The efficacy test for controlling annual weeds in direct-seeded rice fields.

[0062] 1 Test conditions.

[0063] 1.1 Crops and cultivated varieties: Rice (variety: Wanjinyou 133).

[0064] 1.2 The selection of weeds for test subjects.

[0065] The dominant weeds in the field mainly include: Echinochloa crusgalli, Leptochloa chinensis, Alopecurus aequalis, Monochoria vaginglis, Cyperus rotundus, etc.

[0066] 1.3 Cultivation conditions.

[0067] The test site is located in Yanwanghu Village, Yanwanghu Town, Hanshou County, Changde City, Hunan Province. Rice is planted all year round, and rice and rapeseed are rotated. The test rice was sown on April 13, 2011, with a sowing rate of 2 kg/mu. The rice seeds were germinated until the rice seeds were soaked up and the rice seeds were soaked in direct seeding. 15 kg of compound fertilizer per mu was used as base fertilizer. The conditions of rice varieties, sowing time, growth, and fertilizer and water management are the same in each plot, and small ridges are built between plots to prevent string irrigation.

[0068] 2 Experimental design and arrangement.

[0069] 2.1 The experimental design of the test agent.

[0070]

[0071] 2.2 Community arrangements.

[0072] The experiment has 9 treatments, each treatment is repeated 4, a total of 36 cells, the cells are randomly arranged, and the cell area is about 80 square meters.

[0073] 2.3 Method of application.

[0074] The test agent was used according to the dosage of each plot, 37% pretilachlor·pyrazosulfuron-methyl·clomazone oil suspension, 60% pretilachlor·pyrazosulfuron-methyl·clomazone water dispersible granules, 38% Pretilachlor, pyrazosulfuron, clomazone WP, 40% pretilachlor slick oil, 10% pyrazosulfuron-methyl WP, 360g/L clomasulfuron microcapsule suspension with water 30 kg /Mu spray, using Li Nong brand HD-400 hand compression sprayer spray. 3% pretilachlor, pyrazosulfuron-methyl and clomazone granules are applied in poisonous soil. Keep the paddy field moist for 7-10 days after application, and then manage it as usual.

[0075] 2.4 Application time and frequency

[0076] Three days after planting (April 16, 2011), the herbicide was applied once, and the artificial weeding area was weeded twice, 15 days after the application (April 31, 2011) and 25 days after the application (2011, respectively). May 10).

[0077] 3 Investigation, recording and measurement methods.

[0078] 3.1 Meteorological and soil data.

[0079] 3.1.1 Meteorological data.

[0080] The test was conducted from April 16, 2011 to May 15, 2011. The day of application was cloudy and there was no rainfall within 24 hours after application. Weather conditions from the day of application to the last investigation of efficacy: 9 days of rainfall, total rainfall of 254.5 mm, average daily temperature of 14-20 ℃, relative humidity of 50-95%.

[0081] 3.1.2 Soil information.

[0082] The soil in the test site is carbonaceous black mud, rich in organic matter, medium fertility, and pH 6.7.

[0083] 3.2 Weed investigation.

[0084] 3.2.1 Investigation time and frequency.

[0085] The survey was conducted 30 days after the application (May 15, 2011), and the survey was conducted once.

[0086] 3.2.2 Investigation method.

[0087] Sampling at five diagonal points in each plot, surveying 1.0 square meters at each point, recording the total number of weeds in each plot and weighing the fresh weight.

[0088] 3.2.3 Calculation method of efficacy:

[0089] .

[0090] 3.3 Investigation of drug damage.

[0091] 3.3.1 Investigation time and frequency.

[0092] The investigation was conducted 15 days and 30 days after application, and the investigation was conducted 2 times in the whole period.

[0093] 3.3.2 Investigation method.

[0094] Visually inspect the rice seedlings for dwarfing, chlorosis and deformity.

[0095] 3.4 Crop yield and quality.

[0096] 3.4.1 Production test time.

[0097] It was carried out on the day of rice harvest (July 23, 2011).

[0098] 3.4.2 Production testing method.

[0099] Using the actual harvesting method, the rice yield of each plot was weighed and converted into yield per mu. The quality of rice was not measured.

[0100] 4 Results and analysis.

[0101] 4.1 Statistical analysis.

[0102] 37% pretilachlor, pyrazosulfuron-methyl, clomazone oil suspension, 60% pretilachlor, pyrazosulfuron-methyl, clomazone water dispersible granules, 38% pretilachlor, pyrazosulfuron-methyl, Clomazone wettable powder, 3% pretilachlor, pyrazosulfuron-methyl and clomazone granules control over 96% of weeds in direct-seeded rice fields, indicating that pretilachlor, pyrazosulfuron-methyl and clomazone The combination of pine has better effect on controlling gramineous weeds, broad-leaved weeds and sedges in direct-seeded rice fields than the single agents of pretilachlor, pyrazosulfuron-methyl and clomazone.

[0103] 37% Pretilachlor·Pyrimsulfuron·Clomasulfuron Oil Suspension Concentrate, 60% Pretilachlor·Pyrimsulfuron·Clomasulfuron Water Dispersible Granules, 38% Pretilachlor·Pyrimsulfuron· The yield retention rate of clomazone wettable powder is over 47.7%, the yield retention rate of 40% pretilachlor slick oil is 25.7%, the yield retention rate of 10% pyrazosulfuron-methyl WP is 33.0%, 360g/L clomazone micro The guaranteed yield rate of vesicle suspension was 39.4%, and the guaranteed yield rate of manual weeding was 13.3%, indicating that the combination of pretilachlor, pyrazosulfuron-methyl, and clomazone can effectively control and protect rice yield from direct-seeded rice fields. The combination of sulfuron-methyl and clomazone has a higher yield than the single agents of pretilachlor, pyrazosulfuron-methyl and clomazone.

[0104] 4.2 Safety evaluation.

[0105] During the test, the rice growth in the area treated with no test agent was adversely affected.

[0106] Table 3. Test results of the combination of pretilachlor, pyrazosulfuron-methyl, and clomazone for controlling weeds in direct-seeded rice fields.

[0107]

[0108] Table 4. Test results of controlling weeds in direct-seeded rice fields with the combination of pretilachlor, pyrazosulfuron-methyl and clomazone.

[0109]