Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Kit and method for detecting CYP19A1 gene polymorphism by pyrophosphoric acid sequencing method

A technology of pyrosequencing and gene polymorphism, which is applied in the field of molecular biology, can solve the problem of loss of enzyme activity and achieve the effect of small sample size and easy operation

Inactive Publication Date: 2012-08-22
CENT SOUTH UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Exemestane is a steroidal AIs with an androgen structure, which competes with androstenedione, a substrate of CYP19A1, and binds irreversibly to the catalytic center of CYP19A1, resulting in loss of enzyme activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit and method for detecting CYP19A1 gene polymorphism by pyrophosphoric acid sequencing method
  • Kit and method for detecting CYP19A1 gene polymorphism by pyrophosphoric acid sequencing method
  • Kit and method for detecting CYP19A1 gene polymorphism by pyrophosphoric acid sequencing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] CYP19A1-pyroF (upstream primer): 5'-TCAAACTCTTGGCCTCTGCTTT-3' (SEQ ID NO.2);

[0030] CYP19A1-pyroR (downstream primer): 5'-TGGCCCATGGCATTTTAGG-3' (SEQ ID NO.3);

[0031] Sequencing primer: 5'-CCAAGCTAGGTGCTATT-3' (SEQ ID NO.4);

[0032] 1. DNA extraction

[0033] 11 The preparation and inspection of reagent materials before the experiment are as follows:

[0034] (1) Check the shelf life of the kit and ensure that ethanol has been added to Wash Buffer 1 and 2, and tick the corresponding mark on the bottle; (2) Isopropanol (if not available, it can be replaced by absolute ethanol) and 75% ethanol ; (3) 1.5mL Eppendorf tubes and various pipette tips within the validity period of autoclaving.

[0035] 1.2 Take out the EDTA anticoagulant tube containing whole blood from the refrigerator at 4°C, and mix it upside down several times;

[0036] 1.3 Make a mark on the 1.5mL Eppendorf tube corresponding to the unique identification of the specimen;

[0037] 1.4 Pipette 900u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kit and method for detecting CYP19A1 gene polymorphism by a pyrophosphoric acid sequencing method. The kit is used for examining the CYP19A1 gene polymorphism, particularly rs4646 (G is greater than T) single nucleotide polymorphism. The kit comprises primers shown as SEQ ID NO. 2-4. According to the kit, accurate, quick and high-flux detection of the CYP19A1 gene polymorphism can be realized, so that individual administration for realizing safe, rational and effective suppression on primer aromatase of the kit is achieved.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a kit and a method for detecting CYP19A1 gene polymorphism by pyrosequencing. Background technique [0002] Aromatase (Aromatase Enzyme) is a member of the cytochrome P450 enzyme superfamily, and the coding gene is CYP19A1, which is located at 15q211. Enzymes necessary for different stages of aldosterone, cortisol, androgen biosynthesis. CYP19A1 can catalyze the conversion of testosterone and androstenedione to estrone and estradiol, and play the final rate-limiting catalytic role in estrogen biosynthesis, so it is also called estrogen synthase. [0003] Aromatase inhibitors (Aromatase inhibitors, AIs) reduce the level of estrogen by inhibiting aromatase, thereby eliminating the stimulating effect of estrogen on tumor growth. Estrogen in postmenopausal women is mainly derived from the aromatization of androgen precursors in peripheral tissues, so this type of drug i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 周宏灏
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com