Lead ion detection test paper and preparation method thereof

Abstract

The invention discloses a lead ion detection test paper and a preparation method thereof. The test paper consists of a bar-type base plate, a sample pad, a glass fiber combination pad, a specifically enveloped nitrocellulose membrane and a water absorption pad, wherein the sample pad, the glass fiber combination pad, the specifically enveloped nitrocellulose membrane and the water absorption pad are lapped on the bar-type base plate in sequence; the glass fiber combination pad is coated with a special bar-type code DNA-nano gold-deoxyribozyme probe; the nitrocellulose membrane is provided with a quality control line C and a detection line T; and pairing can be formed between a basic group capturing DNA and bar-type code DNA basic group on the surface of the special bar-type code DNA-nano gold-deoxyribozyme probe. The preparation method comprises the following steps: the prepared sample pad, the glass fiber combination pad coated with the special bar-type code DNA-nano gold-deoxyribozyme probe, the specifically enveloped nitrocellulose membrane and the water absorption pad are lapped, are pasted on the base plate and are cut into a thin strip with the width of 3-5mm to obtain the lead ion detection test paper. The lead ion detection test paper has the advantage of capability of detecting the lead ion in the detection sample in high flexibility and high selectivity.

Description

technical field

[0001] The invention relates to the technical field of heavy metal ion detection, in particular to a lead ion detection test strip and a preparation method thereof. Background technique

[0002] Lead (Pb), one of the heavy metals, remains in the organism and the environment for a long time, causing serious damage to the human nervous system and posing a serious threat to human health. Lead in nature is mainly in the form of ions (Pb 2+ ) exists, so it is very important to realize accurate, sensitive and rapid on-site detection of lead ions.

[0003] At present, the methods for detecting lead ions mainly include: ultraviolet-visible spectrophotometry, atomic emission spectrometry, ICP-MS method, atomic absorption spectrometry, electrochemical method, ion chromatography, capillary electrophoresis, heavy metal rapid detector method, test method, etc. Paper strip method, etc. UV-visible spectrophotometry has poor selectivity and high detection limit; atomic em...

Examples

Embodiment 1

[0042] The present invention is a lead ion detection test strip, the test strip consists of a strip base plate 1 and a sample pad 2 sequentially overlapped on the strip base plate 1, coated with a specific barcode DNA-nano gold-deoxyribozyme probe Composed of glass fiber binding pad 3, specific coated nitrocellulose membrane 4, and water-absorbing pad 5, the specific barcode DNA-nanogold-DNAzyme probe is bound to biotin at one end of the DNAzyme, and its other end is bound to There are nano-gold, and several barcode DNAs are combined on the surface of the nano-gold. The linear quality control line C line 6 is coated with streptavidin on the nitrocellulose membrane and bound with biotinylated capture DNA and streptavidin. The complex is coated with a linear detection line T line 7, and the base of the captured DNA is complementary to the base of the barcode DNA on the surface of the specific barcode DNA-nanogold-deoxyribozyme probe,

[0043] The structure of the specific barcod...

Embodiment 2

[0051] A kind of preparation method of lead ion detection test strip of the present invention, concrete steps are as follows:

[0052] 1. Preparation of sample pad 2

[0053] Soak the glass fiber paper with 0.01-0.05 mol / L Tris-HCl buffer solution to wet it, and then dry it at 20°C-37°C for 4-12 hours to obtain a sample pad. The mass fraction of sucrose contained in the Tris-HCl buffer solution is 1~10%, pH value is 5.0~8.0.

[0054] 2. Preparation of glass fiber binding pad 3 coated with specific barcode DNA-nanogold-deoxyribozyme probe

[0055] Soak the glass fiber paper with 0.01-0.05 mol / L Tris-HCl buffer solution (the mass fraction of Tris-HCl buffer solution containing sucrose is 1-10%, and the pH value is 5.0-8.0). After drying at 37°C for 4 to 12 hours, evenly spray 2.0 to 10.0 μL of specific barcode DNA-nanogold-deoxyribozyme probe solution on each square centimeter of glass fiber paper, and dry at 20°C to 37°C for 2 After ~5 hours, the glass fiber binding pad 3 co...

Embodiment 3

[0065] The present invention is a lead ion detection test strip, the test strip consists of a strip base plate 1 and a sample pad 2 sequentially overlapped on the strip base plate 1, coated with a specific barcode DNA-nano gold-deoxyribozyme probe Composed of glass fiber binding pad 3, specific coated nitrocellulose membrane 4, and water-absorbing pad 5, the specific barcode DNA-nanogold-DNAzyme probe is bound to biotin at one end of the DNAzyme, and its other end is bound to There are nano-gold, barcode DNA is combined on the surface of nano-gold, and streptavidin is used to coat the linear quality control line C line 6 on the nitrocellulose membrane, and the complex of biotinylated capture DNA and streptavidin is used. Object-coated linear detection line T line 7, the base of the captured DNA is complementary to the base of the barcode DNA on the surface of the specific barcode DNA-nanogold-deoxyribozyme probe, and its specific preparation is as follows:

[0066] 1. Preparat...

technical field

[0001] The invention relates to the technical field of heavy metal ion detection, in particular to a lead ion detection test strip and a preparation method thereof. Background technique

[0002] Lead (Pb), one of the heavy metals, remains in the organism and the environment for a long time, causing serious damage to the human nervous system and posing a serious threat to human health. Lead in nature is mainly in the form of ions (Pb 2+ ) exists, so it is very important to realize accurate, sensitive and rapid on-site detection of lead ions.

[0003] At present, the methods for detecting lead ions mainly include: ultraviolet-visible spectrophotometry, atomic emission spectrometry, ICP-MS method, atomic absorption spectrometry, electrochemical method, ion chromatography, capillary electrophoresis, heavy metal rapid detector method, test method, etc. Paper strip method, etc. UV-visible spectrophotometry has poor selectivity and high detection limit; atomic em...

Examples

Embodiment 1

[0042] The present invention is a lead ion detection test strip, the test strip consists of a strip base plate 1 and a sample pad 2 sequentially overlapped on the strip base plate 1, coated with a specific barcode DNA-nano gold-deoxyribozyme probe Composed of glass fiber binding pad 3, specific coated nitrocellulose membrane 4, and water-absorbing pad 5, the specific barcode DNA-nanogold-DNAzyme probe is bound to biotin at one end of the DNAzyme, and its other end is bound to There are nano-gold, and several barcode DNAs are combined on the surface of the nano-gold. The linear quality control line C line 6 is coated with streptavidin on the nitrocellulose membrane and bound with biotinylated capture DNA and streptavidin. The complex is coated with a linear detection line T line 7, and the base of the captured DNA is complementary to the base of the barcode DNA on the surface of the specific barcode DNA-nanogold-deoxyribozyme probe,

[0043] The structure of the specific barcod...

Embodiment 2

[0051] A kind of preparation method of lead ion detection test strip of the present invention, concrete steps are as follows:

[0052] 1. Preparation of sample pad 2

[0053] Soak the glass fiber paper with 0.01-0.05 mol / L Tris-HCl buffer solution to wet it, and then dry it at 20°C-37°C for 4-12 hours to obtain a sample pad. The mass fraction of sucrose contained in the Tris-HCl buffer solution is 1~10%, pH value is 5.0~8.0.

[0054] 2. Preparation of glass fiber binding pad 3 coated with specific barcode DNA-nanogold-deoxyribozyme probe

[0055] Soak the glass fiber paper with 0.01-0.05 mol / L Tris-HCl buffer solution (the mass fraction of Tris-HCl buffer solution containing sucrose is 1-10%, and the pH value is 5.0-8.0). After drying at 37°C for 4 to 12 hours, evenly spray 2.0 to 10.0 μL of specific barcode DNA-nanogold-deoxyribozyme probe solution on each square centimeter of glass fiber paper, and dry at 20°C to 37°C for 2 After ~5 hours, the glass fiber binding pad 3 co...

Embodiment 3

[0065] The present invention is a lead ion detection test strip, the test strip consists of a strip base plate 1 and a sample pad 2 sequentially overlapped on the strip base plate 1, coated with a specific barcode DNA-nano gold-deoxyribozyme probe Composed of glass fiber binding pad 3, specific coated nitrocellulose membrane 4, and water-absorbing pad 5, the specific barcode DNA-nanogold-DNAzyme probe is bound to biotin at one end of the DNAzyme, and its other end is bound to There are nano-gold, barcode DNA is combined on the surface of nano-gold, and streptavidin is used to coat the linear quality control line C line 6 on the nitrocellulose membrane, and the complex of biotinylated capture DNA and streptavidin is used. Object-coated linear detection line T line 7, the base of the captured DNA is complementary to the base of the barcode DNA on the surface of the specific barcode DNA-nanogold-deoxyribozyme probe, and its specific preparation is as follows:

[0066] 1. Preparat...

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