Lead ion detection test paper and preparation method thereof

A technology for detecting test strips and lead ions, which is applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of low sensitivity, high analysis cost, poor selectivity, etc., and achieve the effect of high sensitivity

Inactive Publication Date: 2012-08-22
NINGBO UNIV
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

UV-visible spectrophotometry has poor selectivity and high detection limit; atomic emission spectrometry, ICP-MS, atomic absorption spectrometry, electrochemical method, ion chromatography, and capillary electrophoresis have high accuracy and sensitivity, but all require The use of specific instruments is expensive, cumbersome to operate, and requires testing personnel to have certain professional knowledge. The analysis cost is high, and it cannot be app

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lead ion detection test paper and preparation method thereof
  • Lead ion detection test paper and preparation method thereof
  • Lead ion detection test paper and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0041] Example 1

[0042] The present invention is a lead ion detection test strip. The test strip consists of a strip-shaped bottom plate 1 and a sample pad 2 which is sequentially overlapped on the strip-shaped bottom plate 1 and coated with a specific barcode DNA-nano-gold-deoxyribozyme probe The specific barcoded DNA-nano-gold-deoxyribozyme probe is composed of a glass fiber binding pad 3, a specially coated nitrocellulose membrane 4, and a water-absorbing pad 5. Biotin is bound to one end of the deoxyribozyme, and the other end is bound to There are nano-gold, several bar coded DNAs are bound on the surface of nano-gold, the linear quality control line C 6 is coated with streptavidin on the nitrocellulose membrane and combined with biotinylated capture DNA and streptavidin The complex is coated with the linear detection line T line 7, and the bases of the captured DNA are complementary paired with the bases of the barcoded DNA on the surface of the specific barcode DNA-nano-...

Example Embodiment

[0050] Example 2

[0051] The method for preparing a lead ion detection test strip of the present invention has the following specific steps:

[0052] 1. Preparation of sample pad 2

[0053] After soaking the glass fiber paper with 0.01~0.05 mol / L Tris-HCl buffer, it is dried at 20℃~37℃ for 4~12 hours to obtain the sample pad. The mass fraction of sucrose in Tris-HCl buffer is 1~10%, pH value is 5.0~8.0.

[0054] 2. Preparation of glass fiber binding pad 3 coated with specific barcode DNA-nano-gold-deoxyribozyme probe

[0055] The glass fiber paper is soaked and moistened with 0.01~0.05 mol / L Tris-HCl buffer (the Tris-HCl buffer contains 1-10% sucrose, and the pH value is 5.0-8.0), and then at 20℃~ After drying for 4-12 hours at 37℃, spray 2.0-10.0 μL of specific barcode DNA-nano-gold-deoxyribozyme probe solution evenly on every square centimeter of glass fiber paper and dry at 20℃~37℃2 After ~5 hours, the glass fiber binding pad 3 coated with specific barcoded DNA-nano-gold-deoxyri...

Example Embodiment

[0064] Example 3

[0065] The present invention is a lead ion detection test strip. The test strip consists of a strip-shaped bottom plate 1 and a sample pad 2 which is sequentially overlapped on the strip-shaped bottom plate 1 and coated with a specific barcode DNA-nano-gold-deoxyribozyme probe The specific barcoded DNA-nano-gold-deoxyribozyme probe is composed of a glass fiber binding pad 3, a specially coated nitrocellulose membrane 4, and a water-absorbing pad 5. Biotin is bound to one end of the deoxyribozyme, and the other end is bound to There is nano-gold, bar code DNA is bound to the surface of nano-gold, the linear quality control line C 6 is coated with streptavidin on the nitrocellulose membrane, and the combination of biotinylated capture DNA and streptavidin combined The substrate is coated with a linear detection line T line 7, and the bases of the captured DNA are complementary paired with the bases of the barcoded DNA on the surface of the specific barcode DNA-n...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses a lead ion detection test paper and a preparation method thereof. The test paper consists of a bar-type base plate, a sample pad, a glass fiber combination pad, a specifically enveloped nitrocellulose membrane and a water absorption pad, wherein the sample pad, the glass fiber combination pad, the specifically enveloped nitrocellulose membrane and the water absorption pad are lapped on the bar-type base plate in sequence; the glass fiber combination pad is coated with a special bar-type code DNA-nano gold-deoxyribozyme probe; the nitrocellulose membrane is provided with a quality control line C and a detection line T; and pairing can be formed between a basic group capturing DNA and bar-type code DNA basic group on the surface of the special bar-type code DNA-nano gold-deoxyribozyme probe. The preparation method comprises the following steps: the prepared sample pad, the glass fiber combination pad coated with the special bar-type code DNA-nano gold-deoxyribozyme probe, the specifically enveloped nitrocellulose membrane and the water absorption pad are lapped, are pasted on the base plate and are cut into a thin strip with the width of 3-5mm to obtain the lead ion detection test paper. The lead ion detection test paper has the advantage of capability of detecting the lead ion in the detection sample in high flexibility and high selectivity.

Description

technical field [0001] The invention relates to the technical field of heavy metal ion detection, in particular to a lead ion detection test strip and a preparation method thereof. Background technique [0002] Lead (Pb), one of the heavy metals, remains in the organism and the environment for a long time, causing serious damage to the human nervous system and posing a serious threat to human health. Lead in nature is mainly in the form of ions (Pb 2+ ) exists, so it is very important to realize accurate, sensitive and rapid on-site detection of lead ions. [0003] At present, the methods for detecting lead ions mainly include: ultraviolet-visible spectrophotometry, atomic emission spectrometry, ICP-MS method, atomic absorption spectrometry, electrochemical method, ion chromatography, capillary electrophoresis, heavy metal rapid detector method, test method, etc. Paper strip method, etc. UV-visible spectrophotometry has poor selectivity and high detection limit; atomic em...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12Q1/44
Inventor 王泽波郭智勇段静马青青郝婷婷杜书平
Owner NINGBO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap