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Immunonephelometry kit for detecting lipid carrier protein related to neutrophils gelatinase and preparation method thereof

A technology for lipocalin and neutrophils, which is applied in biological tests, measuring devices, material testing products, etc., can solve problems such as the advent of carrier protein detection kits, and achieves that it is not easy to interfere with human factors, simple to operate, and high sensitivity. Effect

Inactive Publication Date: 2012-09-12
苏州照康生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, the latex-enhanced immunoturbidimetric method has been widely used in the detection of cystatin C, lipoprotein a, C-reactive protein, and antistreptolysin 0, and has achieved good social benefits. Neutrophil-like gelatinase-associated lipocalin detection kit released

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of latex suspension: mix latex microspheres with the coated neutrophil gelatinase-related lipocalin monoclonal antibody in phosphate buffer, rotate at 30-40 degrees overnight, and wash with the cleaning stock solution. The latex suspension is stored in an environment of 2~8 degrees; the particle size of the latex microspheres is 100nm; the pH of the phosphate buffer is 7.2, the mass ratio is 30mmol / L; the pH of the cleaning storage solution is 7.4, clean and store The components and weight percentages of the solution are: surfactant 0.2%, preservative 0.05%, buffer 30mmol / L, surfactant is Triton-X405, preservative is Proclin-300, buffer is glycine; latex microspheres The mixing with the coated neutrophil gelatinase-related lipocalin monoclonal antibody is an equal volume mixing.

[0028] Prepare the application solution: Dissolve the surfactant, preservative, sodium chloride and buffer in water to obtain the application solution; among them, the surfactant is 0...

Embodiment 2

[0031] Preparation of latex suspension: mix latex microspheres with the coated neutrophil gelatinase-related lipocalin monoclonal antibody in phosphate buffer, rotate at 30-40 degrees overnight, and wash with the cleaning stock solution. The latex suspension is stored at 2~8 degrees; the particle size of the latex microspheres is 500nm; the pH of the phosphate buffer is 9.2, the mass ratio is 60mmol / L; the pH of the cleaning storage solution is 9.0, cleaning and storage The components of the solution and their weight percentages are: surfactant 0.4%, preservative 0.2%, buffer 60mmol / L, surfactant is Triton-X100, preservative is sodium azide, and buffer is trihydroxyaminomethane ; The latex microspheres and the coated neutrophil gelatinase-related lipocalin monoclonal antibody are mixed in equal volume.

[0032] Prepare the application solution: Dissolve the surfactant, preservative, sodium chloride and buffer in water to obtain the application solution; among them, 0.4% surfactan...

Embodiment 3

[0035] Preparation of latex suspension: mix latex microspheres with the coated neutrophil gelatinase-related lipocalin monoclonal antibody in phosphate buffer, rotate at 30-40 degrees overnight, and wash with the cleaning stock solution. The latex suspension is stored at 2~8 degrees; the particle size of the latex microspheres is 200nm; the pH of the phosphate buffer is 8.0, the mass ratio is 120mmol / L; the pH of the cleaning storage solution is 8.4, clean and store The components and weight percentages of the solution are: surfactant 0.6%, preservative 0.4%, buffer 120mmol / L, surfactant is Tween-20, preservative is a mixture of sodium azide and Proclin-300, and buffer is Carbonate; latex microspheres and coated neutrophil gelatinase-related lipocalin monoclonal antibody are mixed in equal volume.

[0036] Prepare the application solution: Dissolve the surfactant, preservative, sodium chloride and buffer in water to obtain the application solution; among them, the surfactant is 0...

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Abstract

The invention relates to an immunonephelometry kit for detecting lipid carrier protein related to neutrophils gelatinase and a preparation method thereof; an application liquid bottle, an antibody suspending liquid bottle and a standard substance bottle are placed in a box body; application liquid is arranged in the application liquid bottle, and comprises the following components of: 0.01 percent to 1 percent of surfactant, 0.01 percent to 0.5 percent of preservative, 1 percent to 20 percent of sodium chloride and 20mmol / l to 200mmol / l of buffer solution; the antibody suspending liquid bottle contains emulsion suspension which is coated with the monoclonal antibody of the lipid carrier protein related to the neutrophils gelatinase, and the emulsion suspension has the following components of: 0.05 percent to 0.5 percent of emulsion which is coated with the monoclonal antibody of the lipid carrier protein related to the neutrophils gelatinase, 0.01 percent to 1 percent of surfactant, 0.01 percent to 0.5 percent of preservative and 20mmol / l to 200mmol / l of buffer solution; and the standard substance bottle contains the standard substances of the lipid carrier protein related to the neutrophils gelatinase. According to the invention, the mass quantitative detection on a biochemical instrument is realized.

Description

technical field [0001] The invention relates to an immunoturbidimetric detection method, in particular to an immunoturbidimetric kit for detecting neutrophil gelatinase-associated lipocalin and a preparation method thereof. Background technique [0002] Neutrophil gelatinase-associated lipocalin rapid detection ELISA kit can be used for human urine, plasma and serum detection. Acute renal failure (ARF) is a common complication of cardiac surgery, nephrotoxicity, and kidney transplantation. [0003] Neutrophil gelatinase-associated lipocalin (neutrophil gelatinase-associated lipocalin, NGAL), also known as Lipocalin-2 (lipocalin-2), is a new member of the lipocalin family, is the Early biomarkers of injury. NGAL not only exists in neutrophils, but also in specific epithelial cells. For example, in the process of ischemic and toxic renal injury, NGAL in renal tubular epithelial cells will increase significantly. Within hours, the levels of NGAL in urine and blood will incre...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577
Inventor 刘照关康英杰
Owner 苏州照康生物技术有限公司
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