Process for extracting active ingredients of American eleutherine rhizome

A technology of active ingredients and extraction process, which is applied to medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, plant raw materials, etc., and can solve problems such as small side effects, single target, and large side effects

Inactive Publication Date: 2012-09-19
NANJING MAIDISEN MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Red onion also has a certain antioxidant activity and blood lipid lowering effect. Red onion acts on different links of cardiovascular diseases through multiple targets, and synergistic treatment has small side effects, which overcomes the shortcomings of single target and large toxic side effects of synthetic drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Get 4kg of dried red onions and grind them into coarse powder. Soak in 20L of 60% ethanol for 30 minutes, heat and reflux in a water bath at 85°C for 2 hours, filter and separate the extract and filter residue; reflux extraction twice, and finally combine the extracts. Recover under reduced pressure to a thick paste, add 3 L of water to suspend; extract with chloroform and n-butanol respectively to obtain 96 g of chloroform layer, 98 g of n-butanol layer, and 104 g of water layer. Using the blast fungus screening system, the chloroform layer with better activity against blast fungus was separated by silica gel column chromatography, medium and low pressure column chromatography and high performance liquid chromatography to obtain compound 1 (3g), compound 2 (5g), and compound 3 (4g). , Compound 4 (500 mg), Compound 5 (20 mg), Compound 6 (35 mg), Compound 7 (25 mg), Compound 8 (6 mg), Compound 9 (22 mg) and Compound 10 (12 mg). Among them, compounds 9 and 10 were isolat...

Embodiment 2

[0018] Get 4kg of dried red onions and grind them into coarse powder. Soak in 20L of 60% ethanol for 30 minutes, heat and reflux in a water bath at 85°C for 2 hours, filter and separate the extract and filter residue; reflux for 3 times, and finally combine the extracts. Recover under reduced pressure to a thick paste, add 3 L of water to suspend; extract with chloroform and petroleum ether respectively to obtain 83 g of chloroform layer, 35 g of petroleum ether layer, and 145 g of water layer. Using the blast fungus screening system, the petroleum ether layer with better activity against blast fungus was separated by silica gel column chromatography, medium and low pressure column chromatography and high performance liquid chromatography to obtain compound 1 (120mg), compound 2 (50mg), compound 3 (2g ), Compound 4 (20 mg), Compound 5 (33 mg), Compound 6 (20 mg), Compound 7 (140 mg), Compound 8 (10 mg), Compound 9 (50 mg). Among them, compound 9 was isolated from this genus f...

Embodiment 3

[0020] Get 4kg of dried red onions and grind them into coarse powder. Soak in 20L of 60% ethanol for 30 minutes, heat and reflux in a water bath at 85°C for 2 hours, filter and separate the extract and filter residue; reflux for 3 times, and finally combine the extracts. Recover under reduced pressure to a thick paste, add 3 L of water to suspend; extract with chloroform, ethyl acetate, and n-butanol respectively to obtain 96 g of chloroform layer, 98 g of ethyl acetate, 98 g of n-butanol layer, and 104 g of water layer. Using the blast fungus screening system, the chloroform layer with better activity against blast fungus was separated by silica gel column chromatography, medium and low pressure column chromatography and high performance liquid chromatography to obtain compound 1 (100 mg), compound 2 (30 mg), and compound 3 (40 mg). , compound 4 (10 mg), compound 5 (3 g), compound 6 (200 mg), compound 7 (45 mg), compound 8 (12 mg), compound 9 (15 mg), compound 10 (60 mg), com...

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PUM

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Abstract

The invention relates to a process for extracting active ingredients of American eleutherine rhizome. The process includes the steps of crushing, extraction, concentration, extraction, separation and the like and is characterized in that a pyricularia oryzae active screen model is combined for determining the best extraction portion of the active ingredients, and active compounds are separated by means of silica gel column chromatography, medium and low pressure column chromatography, high performance liquid chromatography and the like. The separation and purification process for the effective portion is applicable to a certain scale of production and stable in qualities. The process for extracting the active ingredients of the American eleutherine rhizome is high in efficiency, high in treatment purity of the obtained active ingredients and strong in drug action.

Description

technical field [0001] The invention relates to the extraction process of active ingredients of shallots to evaluate its effect on treating cardiovascular diseases. It belongs to the field of biomedicine. Background technique [0002] Shallot is a plant of the genus Shallot in the family Iridaceae, which is a folk herbal medicine; it is also known as red onion and small red garlic; the medicinal part is the whole plant and the bulb. Red onion is bitter and cool, and it is mainly used for rheumatic joint pain, bruises, sores, hematemesis, hemoptysis, dysentery and amenorrhea abdominal pain. So far, only a dozen compounds have been isolated from shallots, including naphthols, naphthones, anthraquinones and glucosides, which are the main active components of shallots, some of which have antibacterial, anti-corrosion and Arterial blood flow, inhibition of human immunodeficiency virus, inhibition of human topoisomerase II and other pharmacological activities. [0003] Red onio...

Claims

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Application Information

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IPC IPC(8): A61K36/88A61P9/00
Inventor 高晓文
Owner NANJING MAIDISEN MEDICAL TECH
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