Method for producing pleurotus nebrodensis polysaccharide by compound enzymatic hydrolysis technology

A technology of compound enzymatic hydrolysis and Linggu polysaccharide, applied in the field of Bailing mushroom polysaccharide preparation, can solve the problems of large loss of active ingredients, low extraction rate, and failure to meet market demand, and achieve functional diversification and high polysaccharide extraction rate , the effect of reducing the burden on the environment

Inactive Publication Date: 2012-09-19
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But up to now, there is no report on the extraction of polysaccharides from Bailing mushroom by compound enzymatic hydrolysis method, and the extraction rate of traditional extraction methods is too low, and the loss of active components is large, which can no longer meet the market demand.

Method used

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  • Method for producing pleurotus nebrodensis polysaccharide by compound enzymatic hydrolysis technology
  • Method for producing pleurotus nebrodensis polysaccharide by compound enzymatic hydrolysis technology

Examples

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Embodiment 1

[0035] A method for producing Bailing mushroom polysaccharide by using compound enzymolysis technology, comprising the steps of:

[0036] (1) Raw material processing: Wash Bailing mushroom as a raw material, drain it, dry it in vacuum, and crush it into 80 mesh Bailing dry powder; this raw material is the waste in the processing of Bailing mushroom, including Bailing deformed mushroom , broken old edges and stalks during production, and remaining Bailing mushroom roots after picking; (2) Compound enzymolysis with cellulase and pectinase: mix Bailing mushroom dry powder with distilled water at a mass ratio of 1:30 After soaking for 30min, the mass concentration is 0.5% dilute hydrochloric acid and adjusted to pH 5.0, adding the compound enzyme of cellulase and pectinase with 2% mass of Bailing mushroom powder, the quality of cellulase and pectinase in the compound enzyme The ratio is 1:1. After the incubation reaction at 70°C for 1.5h, the temperature is immediately raised to 9...

Embodiment 2

[0041] A method for producing Bailing mushroom polysaccharide by using compound enzymolysis technology, comprising the steps of:

[0042] (1) Raw material processing: wash the remaining Bailing mushroom root as raw material, drain it, dry it in vacuum, and crush it into 100 mesh Bailing mushroom dry powder; this raw material is the waste in the processing of Bailing mushroom, including Bailing mushroom Deformed mushrooms, old edges and stalks broken during production, and leftover roots of Bailing mushrooms after picking;

[0043] (2) Composite enzymatic hydrolysis with cellulase and pectinase: mix Bailing mushroom dry powder with distilled water at a mass ratio of 1:10, soak for 60 minutes, adjust the pH to 6.0 with 0.5% dilute hydrochloric acid, add Bailing A compound enzyme with 1% mushroom powder mass, the mass ratio of cellulase and pectinase in the compound enzyme is 2:1, heat at 60°C for 3 hours, then immediately heat up to 95°C to inactivate the enzyme, and continue to...

Embodiment 3

[0047] A method for producing Bailing mushroom polysaccharide by using compound enzymolysis technology, comprising the steps of:

[0048] (1) Raw material processing: Wash the old edge or stalk of the broken Bailing mushroom, drain it, dry it in vacuum, and crush it into 80 mesh Bailing mushroom dry powder; this raw material is the waste in the processing of Bailing mushroom, including Bailing mushroom Deformed mushrooms, old edges and stalks broken during production, and leftover roots of Bailing mushrooms after picking;

[0049] (2) Composite enzymatic hydrolysis with cellulase and pectinase: mix Bailing mushroom dry powder with distilled water at a mass ratio of 1:20, soak for 45 minutes, adjust the pH to 7.0 with 0.5% dilute hydrochloric acid, add Bailing The compound enzyme with 0.5% mushroom powder mass, in which the mass ratio of cellulase and pectinase is 3:1, put it at 50°C for 2.5 hours, heat it up to 100°C to kill the enzyme, and continue to soak in a water bath at ...

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Abstract

The invention discloses a method for producing pleurotus nebrodensis polysaccharide by a compound enzymatic hydrolysis technology. According to the method, fresh pleurotus nebrodensis raw materials are subjected to slicing, vacuum drying and dry powder grinding; the dry powder and distilled water are mixed according to a mass ratio being 1:10-1:30, after the soaking is carried out, compound enzyme accounting for 0.5 to 2 percent of the mass of the pleurotus nebrodensis power is added, the compound enzyme is formed by mixing cellulose and pectinase according to a mass ratio being 1:1-3:1, the heat insulation reaction is carried out at 50 DEG C to 70 DEG C, then, the enzyme inactivation is carried out, and the leaching is carried out for 0.5 to 2 hours in water bath with the temperature being 95 to 100 DEG C; and centrifugation, supernatant taking, concentration and protein removal are carried out, deproteinized enzymolysis concentrated liquid, and white pleurotus nebrodensis polysaccharide are obtained after precipitation and drying. The method has the advantages that polysaccharide is extracted from wastes in the pleurotus nebrodensis picking and processing process, the wastes are fully used, the mass industrial production is favorably realized, and in addition, a low-energy-consumption and efficient pleurotus nebrodensis polysaccharide extraction method is found.

Description

technical field [0001] The invention relates to a preparation process of Bailing mushroom polysaccharide, in particular to a method for producing Bailing mushroom polysaccharide by using compound enzymolysis technology. Background technique [0002] Bailing mushroom (Pleurotus eryngii var. nebroden-sis), also known as white Ferulicum, belongs to the order Agaricaceae, Pleurotaceae, and Pleurotus genus. It is a white variant of Pleurotus eryngii. , its meat is thick, delicate, crisp and tender, delicious, large in size, good in taste, and highly commercial. According to analysis, Bailing mushroom contains 14.7% protein, 4.31% fat, 15.4% crude fiber, and 10.6% total amino acid. In addition, it also contains linoleic acid, unsaturated fatty acid, polysaccharides, calcium, iron, zinc, manganese, etc. A variety of mineral elements and rich in vitamin C, E, etc., is a rare edible fungus with high edible and medicinal value. It is rich in fungal polysaccharides, which can enhance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 齐军茹廖劲松
Owner SOUTH CHINA UNIV OF TECH
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