Self-assembled modified polylactic acid material of lysozyme and mushroom polysaccharide sulfate and preparation method thereof

A technology of polylactic acid materials and lentinan, which is applied in the field of biomedical materials and biomacromolecular self-assembly, can solve the problems of lack of bioactive groups on the surface of polylactic acid materials, reduced biocompatibility, and no antibacterial properties. Achieve the effect of maintaining natural conformation and biological activity, improving biocompatibility, and good antibacterial activity

Inactive Publication Date: 2012-09-19
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the lack of bioactive groups on the surface of polylactic acid materials reduces its compatibility with organisms
In addition, ordinary biomaterials themselves do not have antibacterial properties. After implantation into the human body, bacteria will adhere and multiply on the surface of the material, which will cause lesions in the body tissue, which is the main reason for the relevant tissue infection and the failure of the implantation operation , 1998, 19(7~9):851~859]

Method used

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  • Self-assembled modified polylactic acid material of lysozyme and mushroom polysaccharide sulfate and preparation method thereof
  • Self-assembled modified polylactic acid material of lysozyme and mushroom polysaccharide sulfate and preparation method thereof
  • Self-assembled modified polylactic acid material of lysozyme and mushroom polysaccharide sulfate and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A kind of preparation method of polylactic acid material modified by lysozyme and lentinan sulfate self-assembly, it comprises the following specific steps:

[0039] 1) Preparation of lentinan sulfate derivatives:

[0040] Add 0.6g lentinan into a reaction flask containing 50mL dimethyl sulfoxide, stir at 20°C for 12 hours, then slowly add 9mL pyridine dropwise at a rate of 2mL / min, continue stirring for 30 minutes, and then the reaction Place the bottle in an ice bath, slowly add 3.7 mL of chlorosulfonic acid (the molar ratio of chlorosulfonic acid to pyridine is 1:2) drop by drop with a constant pressure funnel at a rate of 0.5 mL / min, and then raise the temperature to 80 Continue to stir the reaction for 100 minutes at ℃, cool to room temperature after the reaction stops, adjust the pH to 7.0 with a NaOH solution with a concentration of 5wt%, and obtain a reaction solution; then inject the obtained reaction solution into a regenerated cellulose dialysis bag (36mm, Mw...

Embodiment 2

[0059] A kind of preparation method of polylactic acid material modified by lysozyme and lentinan sulfate self-assembly, it comprises the following specific steps:

[0060] 1) Preparation of lentinan sulfate derivatives:

[0061] Add 1.2g lentinan into a reaction flask containing 100mL dimethyl sulfoxide, stir at 30°C for 18 hours, then slowly add 18mL pyridine dropwise at a rate of 4mL / min, continue stirring for 40 minutes, and then the reaction Place the bottle in an ice bath, slowly add 7.4mL of chlorosulfonic acid (the molar ratio of chlorosulfonic acid to pyridine is 1:2) dropwise with a constant pressure funnel at a rate of 1mL / min under stirring, and then heat up to 80°C Continue to stir the reaction for 120 minutes, cool to room temperature after the reaction stops, adjust the pH to 7.0 with 5wt% NaOH to obtain a reaction solution; then inject the obtained reaction solution into a regenerated cellulose dialysis bag (36mm, Mw: 8000-14000) , dialyzed in NaOH solution wi...

Embodiment 3

[0073] A kind of preparation method of polylactic acid material modified by lysozyme and lentinan sulfate self-assembly, it comprises the following specific steps:

[0074] 1) Preparation of lentinan sulfate derivatives:

[0075] Add 0.6g lentinan into a reaction flask containing 50mL dimethyl sulfoxide, stir at 25°C for 16 hours, then slowly add 18mL pyridine dropwise at a rate of 3mL / min, continue stirring for 35 minutes, and then the reaction Place the bottle in an ice bath, slowly add 3.7mL of chlorosulfonic acid (the molar ratio of chlorosulfonic acid to pyridine is 1:4) dropwise with a constant pressure funnel at a rate of 1mL / min under stirring, and then heat up to 80°C Continue to stir the reaction for 120 minutes, cool to room temperature after the reaction stops, adjust the pH to 7.0 with 10wt% NaOH to obtain a reaction solution; then inject the obtained reaction solution into a regenerated cellulose dialysis bag (36mm, Mw: 8000-14000) , dialyzed in a NaOH solution ...

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Abstract

The invention belongs to the technical field of self assembling of the biomedical material and the biomacromolecule, and particularly relates to a self-assembled modified polylactic acid material of lysozyme and mushroom polysaccharide sulfate and a preparation method thereof. The self-assembled modified polylactic acid material of the lysozyme and the mushroom polysaccharide sulfate comprises base material and a modification layer, wherein the base material comprises surface amination polylactic acid material; the modification layer is obtained by carrying out surface modification on the surface of the base material by the layer-by-layer self-assemble technology; and the modification layer contains the lysozyme and the mushroom polysaccharide sulfate which are self-assembled alternately layer by layer on the surface of the base material. The surface of the polylactic acid material prepared by the method has good antibacterial activity for inhibiting staphylococcus aureus and escherichia coli, and the blood compatibility of the surface of the material is improved. Meanwhile, the preparation method for the material has the advantages of simple technology, moderate preparation condition and low cost and is easy to control.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and biomacromolecule self-assembly, in particular to a polylactic acid material self-assembled and modified by lysozyme and lentinan sulfate and a preparation method thereof. Background technique [0002] At present, polylactic acid materials have become one of the most valued materials in biomedical materials, and are widely used in the field of biomedical materials such as tissue engineering scaffolds, medical sutures, and drug controlled release carriers. However, the lack of biologically active groups on the surface of PLA materials reduces its compatibility with organisms. In addition, ordinary biomaterials do not have antibacterial properties. After implanted into the human body, bacteria will adhere and multiply on the surface of the material, which will cause lesions in the body tissue, which is the main reason for the relevant tissue infection and the failure of the implantat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08J5/18C08L67/04C08B37/00A61L27/40A61L27/54
Inventor 陈艳军连鑫鑫王艺峰王燕梅熊燕飞
Owner WUHAN UNIV OF TECH
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