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Denatured protein powder and brain protein hydrolyzate prepared from same

A technology of cerebroprotein hydrolyzate and protein powder, which is applied in the field of denatured protein powder and cerebroprotein hydrolyzate prepared by using the protein powder

Active Publication Date: 2012-10-10
HEBEI ZHITONG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, to solve the above-mentioned problems, the key lies in controlling the total nitrogen content of the denatured protein (above 120 mg / g) during the preparation process, and exploring suitable preparation processes and conditions, so as to obtain brain protein with amino acid content meeting the national standards for injections and high polypeptide content. Protein hydrolyzate, and the relevant literature in this area has not been reported

Method used

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  • Denatured protein powder and brain protein hydrolyzate prepared from same
  • Denatured protein powder and brain protein hydrolyzate prepared from same
  • Denatured protein powder and brain protein hydrolyzate prepared from same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Preparation of denatured protein powder:

[0115] The first step brain tissue homogenate

[0116] Appropriate amount of fresh pig brain tissue, washed with water to remove blood. Add 0.5g / kg lysozyme to mix and stir together, and colloid mill to homogenize. After 6 times of homogenization, the primary mixture was obtained.

[0117] The second step of protein denaturation

[0118] The above-mentioned brain tissue was heated and boiled for 1 h, and then cooled to room temperature. Add 20 times the amount (weight) of acetone therein, stir for 1 h, filter, and dry at room temperature to obtain denatured protein powder, with a total nitrogen content of 135 mg / g. Store brain protein powder below -20°C.

[0119] Preparation of brain protein hydrolyzate:

[0120] The third step of enzymatic hydrolysis

[0121] (1) Take the above protein powder, according to the total nitrogen content, dissolve the protein powder with purified water to a concentration of 3 mg / mL total nit...

Embodiment 2

[0131] Preparation of denatured protein powder:

[0132] The first step brain tissue homogenate

[0133]Appropriate amount of fresh pig brain tissue, washed with water to remove blood. Add 0.2g / kg lysozyme and mix together, homogenize with a homogenizer. After 4 times of homogenization, the primary mixture was obtained.

[0134] The second step of protein denaturation

[0135] The above brain tissue was heated and boiled for 1 h. Let cool to room temperature. 10 times the amount (weight) of acetone was added thereto. Stir for 1h. filter. Dry at room temperature. The denatured protein powder was obtained, containing 123 mg / g of total nitrogen. Store brain protein powder below -20°C.

[0136] Preparation of brain protein hydrolyzate:

[0137] The third step of enzymatic hydrolysis

[0138] (1) According to the total nitrogen content of the protein powder, dissolve the protein powder with purified water to a concentration of 3 mg / mL total nitrogen content, heat and bo...

Embodiment 3

[0147] Preparation of denatured protein powder:

[0148] The first step brain tissue homogenate

[0149] Appropriate amount of fresh pig brain tissue, washed with water to remove blood. Add 0.8g / kg lysozyme to mix and stir together, and colloid mill to homogenize. After 5 times of homogenization, the primary mixture was obtained.

[0150] The second step of protein denaturation

[0151] The above brain tissue was heated and boiled for 1 h. Let cool to room temperature. Add 20 times the amount (weight) of acetone therein, stir for 1 h, filter, and dry at room temperature to obtain denatured protein powder, with a total nitrogen content of 135 mg / g. Store brain protein powder below -20°C.

[0152] Preparation of brain protein hydrolyzate:

[0153] The third step of enzymatic hydrolysis

[0154] (1) Take the above protein powder, according to the total nitrogen content, dissolve the protein powder with purified water to a concentration of 3 mg / mL total nitrogen content, h...

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Abstract

The invention relates to a denatured protein powder and a brain protein hydrolyzate prepared from the same. The total nitrogen content of the denatured protein powder is more than 120 mg / g; and the denatured protein powder is prepared by adding fresh lysozyme into a pig brain, homogenating, heating, and defatting with acetone. The denatured protein powder, as a raw material, is used for preparingthe brain protein hydrolyzate; the contents of amino acids in the prepared brain protein hydrolyzate meet national standards of brain protein hydrolyzate injections; and the content of small moleculepeptides with the molecular weight of less than 10000 Da is 25-35% of the brain protein hydrolyzate. The preparation method of the brain protein hydrolyzate mainly comprises the following steps: pre-treating, homogenizing, defatting with the acetone, performing dual enzymatic hydrolysis, centrifugating, refining by column chromatography, and performing ultrafiltration treatment. The invention discloses key technical points for controlling the total nitrogen index of the protein powder and the content of the small molecule peptides for the first time; the obtained brain protein hydrolyzate is a biologically active substance which is really extracted from the pig brain, without adding active components artificially, thus ensuring the efficacy and the safety of the brain protein hydrolyzate;and animal pharmacodynamic experiments show that the brain protein hydrolyzate is more effective than conventional products.

Description

technical field [0001] The invention belongs to the technical field of preparation of biochemical medicines, and relates to a method for preparing active brain polypeptide substances and an active brain polypeptide product prepared by the method, more specifically, a denatured protein powder and a protein powder prepared by using the protein powder. Brain protein hydrolyzate. Background technique [0002] Cerebroprotein hydrolyzate drugs are one of the most widely used biochemical drugs in recent years. It occupies an important position in related research fields and markets at home and abroad. Cerebroprotein hydrolyzate, as an active brain polypeptide substance extracted from healthy and fresh animal brain tissue by multi-enzyme hydrolysis, contains a variety of essential amino acids for human brain and important elements such as cephalin, lecithin, and peptide nerve growth factor. It acts on the central nervous system in various ways, regulates and improves the metabolis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/14C12P21/06C12P13/04A61K38/01A61P25/28A61P25/00A61P31/00A61P31/04A61P25/24A61P25/18A61P9/10
Inventor 刘衡徐顺意任保华陈雪艳汤兰芝张敏夏彤
Owner HEBEI ZHITONG BIOLOGICAL PHARMA
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