Method for preparing cerebroprotein hydrolysate by using non-solvent degreasing

Abstract

The invention discloses a method for preparing cerebroprotein hydrolysate by using non-solvent degreasing, which comprises the following steps of (1) tissue homogenate; (2) degreasing; (3) cleaning and filtering; (4) enzymolysis of pepsin; (5) enzymolysis of trypsin; and (6) ultrafiltration. The degreasing mode is implemented by carrying out enzymolysis by using phospholipase and lipase, so that the problem of solvent residues and environment hazards due to the adoption of organic solvent degreasing in existing methods is solved, and the problem that the polypeptide content of cerebroprotein hydrolysate is low is unexpectedly solved; and the polypeptide content of cerebroprotein hydrolysate prepared according to the method is 90% or over, and accords with national standards.

Description

technical field [0001] The invention relates to the fields of medicine and health food, in particular to a preparation method of cerebroprotein hydrolyzate. Background technique [0002] Cerebroprotein hydrolyzate is an active polypeptide obtained by enzymatically hydrolyzing animal brain tissue. It can act on the central nervous system in various ways, regulate and improve the metabolism of neurons, promote the formation of synapses, and induce the differentiation of neurons. And further protect nerve cells from various ischemia and neurotoxin damage. [0003] The basic production process of cerebroprotein hydrolyzate is as follows: After removing mucous membranes and blood stains on the surface of animal brain tissue, add water to homogenate, degrease, pepsin enzymolysis, trypsin enzymolysis, ultrafiltration. [0004] Degreasing is not a necessary step in this process, and brain tissue homogenization can also be directly enzymatically hydrolyzed, such as Chinese patents 2...

AI Technical Summary

Problems solved by technology

[0004] Degreasing is not a necessary step in this process, and brain tissue homogenization can also be directly enzymatically hydrolyzed, such as Chinese patents 200410073403.5 and 201110227333.4, there are three major defects in direct enzymatic hydrolysis without degreasing. The yield and content decrease, and the product is mixed with large molecular weight allergens; second, the product has a high fat content, which affects the stability and appearance of the product; third, it is difficult to filter in the subsequent process

[0005] Therefore, degreasing brain tissue homogenate helps to improve product quality and yield. Acetone or acetone composite solvent degreasing is the most common degreasing method, such as Chinese patent 200410043939.2, 94104516.1. The harm of this degreasing method is that a large amount of organic solvents are used , causing environmental hazards and solvent residues

In order to avoid solvent degreasing, Chinese patent 201310113215.X uses a milk fat separator plus diatomaceous earth for degreasing, avoiding the use of organic solvents, but this method of degreasing results in a decrease in the yield of active peptides and increases equipment investment costs

Chinese patent 201210242784.X uses carbon dioxide supercritical extraction method to degrease, which does not cause environmental hazards, but the supercritical extraction equipment is huge, the price is high, and the output is extremely low, which limits industrial applications

[0006] In addition, the brain protein hydrolyzate prepared by the production process in the prior art has a low nitrogen content. According to the national drug standard WS1-XG-025-2000, the nitrogen content of the dried substance is 8%-10%, which is converted into amino acid + polypeptide The content is about 60%, and the potential clinical risk caused by 40% unknown non-amino acid + polypeptide substances can be imagined. Therefore, the State Food and Drug Administration has continuously improved the standards for such products, and gradually eliminated outdated products.

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