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Method for simultaneously extracting amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin from eggs

A technology of amoxicillin acid and amoxicillin, which is applied in the field of veterinary drug residue detection, can solve the problems that amoxicillin and amoxicillin acid cannot be extracted, and achieve the effects of simplifying sample extraction steps, improving work efficiency, and improving recovery rate

Inactive Publication Date: 2012-10-10
YANGZHOU UNIV
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Problems solved by technology

[0003] In order to solve the problem that the prior art cannot simultaneously extract amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin from eggs, the present invention provides a method for simultaneous extraction and purification, which is simple, Convenient, fast, high recovery rate

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  • Method for simultaneously extracting amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin from eggs
  • Method for simultaneously extracting amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin from eggs
  • Method for simultaneously extracting amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin from eggs

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Embodiment Construction

[0010] The percentage concentration of the reagent involved in the present invention refers to the mass percentage concentration unless otherwise specified.

[0011] 1. Feeding, drug administration and sample collection of experimental chickens

[0012] Randomly select 25 laying hens of 28-week-old Jinghai Yellow Chicken, weighing 1.78±0.22kg, as the blank control group, and raise them in single cages. Pre-feeding for 1 week before the experiment, fed the full-price feed without any antibacterial drugs (made in Jiangsu Jinghai Poultry Group Co., Ltd. feed factory), and had free access to water. Feed the full-price feed without any antibacterial drugs for 5 consecutive days, collect eggs from 17:30 to 18:00 every day, and store egg samples in a low-temperature egg bank.

[0013] 2. Extraction, extraction and purification steps of the present invention:

[0014] ⑴ Homogenize the eggs with a homogenizer;

[0015] (2) Accurately weigh 5.0-6.0g egg homogenate sample, put it in a...

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Abstract

The invention relates to a method for simultaneously extracting amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin from eggs. The method comprises the following steps: adding a penicillin V internal label solution and then acetonitrile into homogenate eggs, mixing for direct extraction, centrifuging, removing the supernatant fluid, repeatedly extracting for 1-2 times, and merging the supernatant fluid to obtain an extract; adding 1ml ammonium acetate solution (pH 6.7) into the extract, placing the extract on a vortex mixer for mixing, adding methylene chloride saturated through ultrapure water, fully oscillating, mixing and centrifuging, moving medicine to the upper-layer water phase through the liquid-liquid extraction procedure, removing the upper-layer water phase, repeatedly extracting for 1-2 times, and then merging with the water phase; and finally drying through a pressure blowing concentrator. The method provided by the invention is simple, convenient, quick and high in recovery rate (the average recovery rate is above 80.15 percent), and the intraday and interday RSDs (Relative Standard Deviations) are respectively 8.45 percent and 10 percent, which conforms to the residue determination requirement of the AOAC (Association of Official Analytical Chemists).

Description

technical field [0001] The invention relates to the field of detection of veterinary drug residues, in particular to a method for simultaneously extracting and purifying amoxicillin, amoxicillin acid, diketopiperazine amoxicillin and ampicillin in eggs. Background technique [0002] In the detection of food residues, if multiple substances can be detected at the same time, the work efficiency will be greatly improved, but only when multiple residues can be extracted from the sample at the same time, can an efficient detection method be established . Both amoxicillin and ampicillin are white or off-white crystalline powders, slightly bitter in taste, slightly soluble in water, and almost insoluble in ethanol. However, the sodium salt or trihydrate of the two is easily soluble in water and slightly soluble in ethanol. Amoxicillin acid and diketopiperazine amoxicillin are the major metabolites of amoxicillin. There are many reports on the extraction methods of amoxicillin an...

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Application Information

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IPC IPC(8): G01N30/06
Inventor 谢恺舟贾龙飞陈学森张小杰谢星孙瑛瑛赵敏李爱华张跟喜戴国俊王金玉
Owner YANGZHOU UNIV
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