Tissue culture method for acer truncatum

A tissue culture, ingot maple technology, applied in the field of plant reproduction, can solve the problems of long seed reproduction growth cycle, high price of ingot maple, restricting the promotion of ingot maple, etc., to achieve good value-added effect, mild environmental stress, and improve rooting. rate effect

Inactive Publication Date: 2012-10-17
江苏汤氏园林有限公司
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Problems solved by technology

Due to its growth characteristics, at present, the maple is mainly propagated by seeds, but the growth cycle of seed propagation is too long, and th

Method used

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Embodiment

[0013] The tissue culture method of a kind of maple ingot of present embodiment comprises: in the first ten days of a month in March, collect the year-old branch bud of the parent tree of maple ingot of 4~6 years old as explant, the explant method such as 4 degree centigrade 50% C Rinse with alcohol for 45-60 seconds, then disinfect with 15% hydrogen peroxide for 4-5 minutes, and then wash with sterile water several times. Then the sterilized explants were taken out, cut into sections on sterile filter paper, each section of the explants was cut into sections with a length of 0.8-1.2 cm, cut into sections and inoculated into the culture medium. The preparation of the culture medium is: MS+2.0mg / L 6-BA+0.6mg / L NAA, adding 35~45g sucrose and 20~25g agar to each liter of the culture medium, so as to obtain from inorganic matter, nitrogen source, carbon source, growth Factors and other aspects meet the conditions for cell induction and differentiation. Tissue induction culture wa...

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Abstract

The invention discloses a tissue culture and breeding method for acer truncatum. The method comprises the following steps: acquiring an annual shoot bud from an acer truncatum mother tree with an age of 4 to 6 years in the first ten days of March as an explant, cutting the shoot bud into sections after sterilization, and inoculating the cut sections to a culture medium of MS + BA + NAA for tissue induction culture, wherein in a culture environment, a pH value is 5.6 to 5.8, temperature is 20 to 24 DEG C, illumination intensity is 1500 lux, 18 hours of each day is for illumination, and 6 hours of each day is for culture in dark. According to the invention, a good value-increasing effect and high quality tissue culture and breeding are realized through constructing a scientific culture medium and assisting the culture medium with reasonable technical means; the growth characteristics of acer truncatum are combined at the same time, stress of the environment is mild during the process of tissue culture, and the rooting rate of the explant is increased.

Description

technical field [0001] The invention relates to the field of plant propagation, in particular to a method for tissue culture of maple ingots. Background technique [0002] Yuanbao Maple Book Acer is a deciduous tree in the Aceraceae family, generally 8-10m high, with longitudinally split bark. Single leaves, opposite, palmate, flowering in May, fruiting in September. Yuanbao maple is a famous autumn ornamental red-leaved tree species, with beautiful tree posture, beautiful leaf shape, red young leaves, which turn yellow and fiery red in autumn, and have high ornamental value. [0003] Yuanbao maple is weakly positive, resistant to half shade, cold, relatively wind resistant, not resistant to dry heat and strong sunlight, and has a medium growth rate. Due to its growth characteristics, at present, the maple is mainly propagated by seeds, but the growth cycle of seed propagation is too long, and the quality difference may be large, which is also the main reason restricting t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 汤榕汤槿
Owner 江苏汤氏园林有限公司
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