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Method for producing microalgae through utilizing livestock and poultry excrement primary wastewater

A livestock and poultry feces and primary technology, applied in the field of microalgae production, can solve complex and high-cost problems, achieve stable operation, increase biomass, and reduce cultivation costs

Active Publication Date: 2012-10-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the culture of microalgae is mainly in synthetic medium, with carbon dioxide as the carbon source, under the conditions of light and mixing; the cost is relatively high; traditionally, microalgae can be used for the deep purification of secondary wastewater, using anaerobic treatment The secondary wastewater of livestock and domestic wastewater is used for microalgae cultivation to overcome the inhibition of high organic matter content, complex flora and water turbidity on the growth of microalgae

Method used

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  • Method for producing microalgae through utilizing livestock and poultry excrement primary wastewater
  • Method for producing microalgae through utilizing livestock and poultry excrement primary wastewater

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Embodiment 1

[0031] 1) The pretreatment method of pig manure primary wastewater: add flocculant chitosan and coagulant sodium alginate to pig manure primary wastewater at a ratio of 10g / L respectively, and carry out flocculation treatment, and the pH of flocculation is 5.0~7.0 between. The obtained supernatant pig manure primary waste liquid was diluted to the initial COD values ​​of 500mg / L, 700mg / L, 800mg / L, and 1000mg / L respectively. Introduce ozone until its concentration reaches above 2.8mg / L, and the sterilization rate reaches above 97% after ozone treatment. Aeration reduces the ozone concentration in the liquid to 0.072mg / L.

[0032] 2) Microalgae culture: inoculate the chlorella cultured to the logarithmic growth phase into the liquid obtained in step 1 at an inoculation amount of 10%, and carry out aerated culture at a temperature of 25°C to 35°C. During the cultivation process, the air of 100mL / min L has been supplied by the air pump, and the introduction of carbon dioxide is ...

Embodiment 2

[0035] The pretreatment method of pig manure primary wastewater: add flocculant chitosan and coagulant sodium alginate to the pig manure primary wastewater at a ratio of 5 g / L, respectively, for flocculation treatment, and the pH of flocculation is between 5.0 and 7.0 between. The obtained supernatant pig manure primary waste liquid was diluted to an initial COD value of 800mg / L. Introduce ozone until its concentration reaches 2.58 mg / L, 2.86 mg / L, and 3.1 mg / L respectively, and test the sterilization rate.

[0036] Such as Figure 5 As shown, the sterilization rate is only about 60% after the ozone concentration is 2.58 mg / L, while the sterilization rate can reach more than 97% after the ozone concentration is 2.86 mg / L or 3.1 mg / L. For the principle of economy, the ozone sterilization of the present invention is preferably passed to an ozone concentration above 2.8mg / L.

Embodiment 3

[0038] 1) The pretreatment method of pig manure primary wastewater: add flocculant chitosan and coagulant sodium alginate to pig manure primary wastewater at a ratio of 8 g / L, respectively, for flocculation treatment, and the pH of flocculation is 5.0~ 7.0 between. The obtained supernatant pig manure was diluted to the initial COD value of 1000 mg / L respectively. Introduce ozone until its concentration reaches above 2.8mg / L, and the sterilization rate reaches above 97% after ozone treatment. Aeration reduces the ozone concentration in the liquid to below 0.1mg / L.

[0039] 2) Microalgae culture: Inoculate the chlorella cultured to the logarithmic growth phase into the liquid obtained in step 1 at an inoculum amount of 15%, and carry out aerated culture at a temperature of 25°C to 35°C. During the cultivation process, the gas has been fed in the following ways: a. Only the air pump is used to continuously feed the air; b. The mixed gas of carbon dioxide and air is continuously...

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Abstract

The invention discloses a method for producing microalgae through utilizing livestock and poultry excrement primary wastewater. The method comprises the following steps: 1, wastewater processing: flocculating the livestock and poultry excrement primary wastewater, taking the resulting supernatant, adding water to dilute to the COD being below 1000mg / L, letting ozone in to carry out aeration disinfection, and allowing the obtained mixture to stand; and 2, microalga culture: inoculating alga strains into the liquid obtained in step 1, carrying out illumination culture, letting air in during the culture process, and letting CO2 in to maintain the pH value of the liquid at 5-7. The preprocessing method of the livestock and poultry excrement primary wastewater allows the microalgae to be cultured through using the primary wastewater without wastewater anaerobic digestion, and complex florae in the wastewater to be removed through ozone disinfection, so the culture system stably runs, the culture cost of the microalgae is reduced, the biomass of the microalgae is improved, and it is in favor of the realization of the scale culture of the microalgae, and the resource utilization of the livestock and poultry excrement wastewater.

Description

technical field [0001] The invention relates to a method for producing microalgae by utilizing the primary waste water of livestock and poultry manure. Background technique [0002] The current world energy system is facing a rapid transition to a low-carbon, efficient, and environmentally friendly energy supply system. However, the shortage of raw materials seriously restricts its scale and industrialization, and raw materials have become the main factor determining the price of biodiesel. It is of great significance to ensure the future development of biodiesel in my country. The use of microalgae resources as the oil source of biodiesel has become a research hotspot in the field of biodiesel. [0003] Microalgae is a kind of living in fresh water, rapid growth and reproduction, large biomass, can use light energy self-support, sustainable supply of biomass resources, the use of livestock and poultry wastewater to breed microalgae is to achieve low-cost industrial product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王海英牟晓庆熊海容朱静静王溢
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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