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Liquid enzyme stabilizing additive and preparation method and application thereof

A liquid enzyme and additive technology, applied in chemical instruments and methods, applications, cosmetic preparations, etc., can solve the problems affecting the production and development of liquid detergent formulations with liquid enzymes, poor stability, loss of vitality, etc.

Inactive Publication Date: 2012-11-07
SHANXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor stability of liquid enzyme preparations with high enzyme activity, especially in liquid enzyme detergents, the existence of water and a large amount of ionic surfactants causes the survival period of liquid enzymes to be less than one month
This is because the enzyme is unstable in the formula, and the enzyme preparation is easily dissolved in the formula and loses its vitality (Michael R. Stoner, Douglas A. Dale, Peter J. Gualfetti, Todd Becker, Enzyme and Microbial Technology 34 (2004) 114- 125), thus seriously affecting the production and development of liquid enzyme formulations, especially liquid detergents

Method used

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  • Liquid enzyme stabilizing additive and preparation method and application thereof
  • Liquid enzyme stabilizing additive and preparation method and application thereof
  • Liquid enzyme stabilizing additive and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Ether nonionic surfactant EO 76 PO 30 EO 76 Dissolve 1g in 10g of deionized water, then take 1g of liquid protease (ATCC2152 alkaline protease fermentation broth) and mix it, add the mixture dropwise to 0.6g of α-cyclodextrin, then add 15g of sodium dodecylbenzene sulfate surfactant (HLB value 10.6) Mix well.

[0021] The above liquid enzyme stabilizing additives were transferred to two vials, sealed and incubated at 5°C and 27°C for 4 weeks respectively. The enzyme activity of protease samples was determined by the national standard GB / T23527-2009 (see Table 1).

[0022] Control: Dissolve 1 g of liquid protease (enzyme liquid produced by alkaline protease fermentation broth ATCC2152) in 10 g of deionized water, then add 15 g of sodium dodecylbenzene sulfate surfactant (HLB value 10.6) and mix evenly. The determination method is the same as above.

[0023] Table 1

[0024]

Embodiment 2

[0026] Ether nonionic surfactant PEO 133 PPOs 50 PEOs 133 Dissolve 0.6g in 15g of deionized water, take 1.2g of liquid α-amylase (Bacillus subtilis) and mix it, add the mixture dropwise to 1.8g of hydroxypropyl-cyclodextrin, and then add 10g of C12 fatty alcohol polyoxyethylene ether 9 (HLB value 13.5) and 10g C12 alkyl glycoside surfactant (HLB value 16) were mixed.

[0027] The above liquid enzyme stabilizing additives were transferred to two vials, sealed and incubated at 5°C and 27°C for 4 weeks respectively. The enzyme activity of amylase samples was determined by the national standard GB8275-2009 method (see Table 2).

[0028] Control: Dissolve 1.2g of liquid amylase protease in 15g of deionized water, then add 10g of C12 fatty alcohol polyoxyethylene ether 9 (HLB value 13.5) and 10g of C12 alkyl glycoside surfactant (HLB value 16) and mix evenly Ditto.

[0029] Table 2

[0030]

Embodiment 3

[0032] Ether nonionic surfactant PEO 27 PPOs 61 PEOs 27 Dissolve 2 g in 10 g of deionized water, mix 1 g of liquid cellulase (1,4-β-D-glucan glucoside hydrolase) with it, add the mixture dropwise to 0.6 g of 15-crown ether-5, and then add 12g C12 fatty alcohol polyoxyethylene ether 3 (HLB value 8.5) and 10g sodium dodecylsulfonate (HLB value 13) surfactant were mixed evenly.

[0033] The above liquid enzyme stabilizing additives were transferred to two vials, sealed and incubated at 5°C and 27°C for 4 weeks respectively. After storage, the enzyme activity of the cellulase samples was determined by QB2583-2003 (the industry standard of the Ministry of Light Industry) (see Table 3).

[0034] Control: Dissolve 1g of liquid amylase protease in 10g of deionized water, then add 12g of C12 fatty alcohol polyoxyethylene ether 3 and 10g of sodium dodecylsulfonate (HLB value 13) surfactant and mix well, and the determination method is the same as above.

[0035] table 3

[0036] ...

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Abstract

The invention provides a liquid enzyme stabilizing additive and a preparation method and application thereof. The liquid enzyme stabilizing additive comprises the following components: liquid enzyme, an ether nonionic surfactant, a linear or branched chain polymer and a surfactant. Compared with the conventional enzyme stabilizer in the prior art, the liquid enzyme stabilizing additive is environment-friendly and easy to degrade. The liquid enzyme stabilizing additive can be used for any composition containing the liquid enzyme and is particularly suitable for washing compositions, personal nursing care compositions, textile treatment compositions and the like, and the liquid enzyme can exert the using efficacy better.

Description

technical field [0001] The invention relates to an enzyme stabilizer, in particular to a liquid enzyme stabilizing additive and a preparation method thereof, as well as the application of the liquid enzyme stabilizing additive in detergents. Background technique [0002] The stability of enzymes in liquids has been a well-known problem, especially in enzyme-containing liquid detergents. Especially in enzymatic detergents, ensuring that enzyme activity does not change over time can be a major challenge. [0003] Traditional enzyme stabilizer systems are mostly composed of borax, propylene glycol and glycerin (Wang Yan, Daily Chemical Science, 2000, 23, 42; Wada Kyoshang, Daily Chemical Industry, 2005, 35, 30), while borax The extensive use of borax has caused great pressure on the environment (it is reported that every ton of borax produced produces 3 tons of boron mud), forming a great environmental pollution, which is not conducive to the tradition of environmental protect...

Claims

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Application Information

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IPC IPC(8): C11D1/83C11D1/835C11D1/94C11D1/825C11D3/386C11D3/37C14C1/08C14C5/00A61K8/86A61K8/66A61K47/34A61K47/40D06L3/11A23K1/165D06L4/40
Inventor 张剑徐淑宜
Owner SHANXI UNIV
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